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A culture medium enriched for Streptococcus faecalis

A technology for Streptococcus faecalis and culture medium, which is applied to the field of culture medium enriching Streptococcus faecalis, can solve the problems of high cost, complex culture medium components, long cycle and the like, and achieves the effects of low cost, high speed and prolonged growth period

Inactive Publication Date: 2011-12-07
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The average enrichment time is 25-30 days, the cycle is long, and there are disadvantages of complex medium components and high cost

Method used

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  • A culture medium enriched for Streptococcus faecalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of Enrichment Medium for Streptococcus faecalis

[0021] Basal medium: Take 10g of peptone, 10g of sodium acetate and 10g of agar powder, and make 1000ml with distilled water.

[0022] In the above basal medium, add 5ml of salt solution (salt solution is: 40g of MgSO 4 ·7H 2 O, 2 g MnSO 4 4H 2 O, 2 g FeSO 4 ·7H 2 O and 2g of NaCl are made into 1000ml), 18g of glucose and 10g of yeast extract with distilled water.

[0023] (2) Culture of Streptococcus faecalis

[0024] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Streptococcus faecalis was inoculated in the medium according to the inoculum concentration of 3%, oscillated and mixed, and cultured in a constant temperature incubator at a temperature of 30°C.

[0025] Enrichment culture results: constant temperature culture for 22 hours, bacterial solution OD 660 1.4, light culture 26h, reach the maximum growth, bacterial solution OD 660 1.5, the maximum growth of S...

Embodiment 2

[0027] (1) Preparation of Enrichment Medium for Streptococcus faecalis

[0028] Basal medium: Take 10g of peptone, 10g of sodium acetate and 10g of agar powder, and make 1000ml with distilled water.

[0029] In the above basal medium, add 3ml of salt solution (salt solution is: 40g of MgSO 4 ·7H 2 O, 2 g MnSO 4 4H 2 O, 2 g FeSO 4 ·7H 2 O and the NaCl of 2g, be made into 1000ml), the glucose of 15g and the yeast extract of 5 with distilled water.

[0030] (2) Culture of Streptococcus faecalis

[0031] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Streptococcus faecalis was inoculated in the medium according to the inoculum concentration of 3%, oscillated and mixed, and cultured in a constant temperature incubator at a temperature of 30°C.

[0032] Enrichment culture results: constant temperature culture for 22 hours, bacterial solution OD 660 1.35, light culture 27h, reach the maximum growth, bacterial solution OD 660 1.5, the maximum ...

Embodiment 3

[0034] (1) Preparation of Enrichment Medium for Streptococcus faecalis

[0035] Basal medium: Take 10g of peptone, 10g of sodium acetate and 10g of agar powder, and make 1000ml with distilled water.

[0036] In the above basal medium, add 7ml salt solution salt solution (salt solution is: 40g of MgSO 4 ·7H 2 O, 2 g MnSO 4 4H 2 O, 2 g FeSO 4 ·7H 2 O and 2g of NaCl are made into 1000ml), 20g of glucose and 15g of yeast extract with distilled water.

[0037] (2) Culture of Streptococcus faecalis

[0038] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Streptococcus faecalis was inoculated in the medium according to the inoculum concentration of 3%, oscillated and mixed, and cultured in a constant temperature incubator at a temperature of 30°C.

[0039] Enrichment culture results: constant temperature culture for 22 hours, bacterial solution OD 660 is 1.5, reaching the maximum growth amount, and advancing the maximum growth amount of Strepto...

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Abstract

The invention relates to a culture medium for enriching Streptococcus faecalis. The technical solution adopted is: a culture medium for enriching Streptococcus faecalis, characterized in that it consists of basal medium, salt solution, glucose and yeast extract; in the basal medium, add 3-7ml per 1000ml of basal medium salt solution, 15-20g of glucose and 5-15g of yeast extract; the salt solution is: 40g of MgSO4.7H2O, 2g of MnSO4.4H2O, 2g of FeSO4.7H2O and 2g of NaCl, with distilled water into 1000ml. The culture medium of the invention is used to cultivate the streptococcus faecalis, and the culture speed is fast and the cost is low. The shortcoming of the slow growth period and high cost of the streptococcus faecalis is made up for, the growth rate is increased, and the time for reaching the maximum growth amount is shortened by 3 to 5 hours.

Description

technical field [0001] The invention relates to a culture medium for enriching bacteria, in particular to a culture medium for enriching Streptococcus faecalis. Background technique [0002] Streptococcus faecalis is a microbial strain that is beneficial to both humans and animals. It is also one of the feed-grade microbial feed additives permitted by the Ministry of Agriculture. It is a facultative anaerobic Gram-positive bacterium that can regulate animal intestinal tract Micro-ecological flora balance, improve immunity and other effects. It can be made into microbial preparations through a series of processes, which can be directly fed to livestock and poultry, which can not only improve the microbial structure in the intestinal tract of livestock and poultry, but also competitively repel other pathogenic microorganisms in the intestinal tract, maintain the micro-ecological balance in the body, and improve the digestive system , promote the absorption of nutrients by liv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 徐成斌付保荣孟雪莲马溪平李法云惠秀娟曹向宇
Owner LIAONING UNIVERSITY
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