Method for symbiotic culture of streptococcus faecalis and saccharomycetes
A technology for symbiotic culture of Streptococcus faecalis, applied in the field of symbiotic culture of facultative anaerobic Streptococcus faecalis and yeast, can solve problems such as pH drop and difficult cultivation of Streptococcus faecalis, achieve good effects, reduce fermentation costs and Time, the effect of increasing the number of viable bacteria
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Embodiment 1
[0015] Embodiment 1 The relationship between the different inoculum sizes of mixed bacteria and the amount of cultured bacteria
[0016] (1) The formula of the medium is: every 1000ml medium contains 24g of peptone, 18g of glucose and 8g of yeast powder.
[0017] (2) Method: According to the inoculation amount of Saccharomyces cerevisiae seed liquid, it is 2%, 2.5%, 3%, 3.5% respectively; according to the inoculation amount of Streptococcus faecalis seed liquid, it is 0.5%, 1.0%, 1.5%, 2.0% respectively ; The initial pH is 7.0, first inoculate the Streptococcus faecalis seed solution into the medium and cultivate for 4 hours, then add the Saccharomyces cerevisiae seed solution, and cultivate for 20 hours at 30°C with the shaking speed of 180rpm. see results figure 1 .
[0018] Saccharomyces cerevisiae and Streptococcus faecalis belong to fungi and bacteria respectively. The size of a single yeast cell is much larger than a single Streptococcus faecalis cell, so the consump...
Embodiment 2
[0019] Example 2 The amount of mixed bacteria in the liquid culture of different initial pH
[0020] (1) The formula of the medium is: every 1000ml medium contains 24g of peptone, 18g of glucose and 8g of yeast powder.
[0021] (2) Method: The inoculation volumes of Saccharomyces cerevisiae and Streptococcus faecalis seed solution were 2.5% and 1.0% respectively; the initial pH was 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 respectively, and the seed solution of Streptococcus faecalis was first inoculated into After culturing in the culture medium for 4 hours, Saccharomyces cerevisiae seed liquid was added, and cultured at 30° C. with a shaking speed of 180 rpm for 20 hours. see results figure 2 with image 3 .
[0022] Saccharomyces cerevisiae is an aerobic bacteria. Streptococcus faecalis is a facultative anaerobic bacterium that figure 2 It can be seen that when the initial pH is 7.0, the number of live bacteria of Saccharomyces cerevisiae and Streptococcus faecalis is highe...
Embodiment 3
[0024] Example 3 The amount of cultured bacteria of Streptococcus faecalis and Saccharomyces cerevisiae in different ways
[0025] (1) The formula of the medium is: every 1000ml medium contains 24g of peptone, 18g of glucose and 8g of yeast powder.
[0026] (2) Method: The inoculum volume of Saccharomyces cerevisiae and Streptococcus faecalis seed solution was 2.5% and 1.0% respectively; the initial pH was 7.0, according to the feeding sequence shown in Table 1, at 30°C, the shaking speed was 180rpm, and cultivated for 20h. The results are shown in Table 1.
[0027]
[0028] It can be seen from Table 1 that Saccharomyces cerevisiae and Streptococcus faecalis were cultured symbiotically, and different inoculation sequences had a significant impact on the final culture effect of these two strains. Streptococcus faecalis is first inoculated into the liquid medium and then cultivated to its pre-log, middle and end-logarithmic stages, and then inoculated with Saccharomyce...
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