Method for symbiotic culture of streptococcus faecalis and saccharomycetes

A technology for symbiotic culture of Streptococcus faecalis, applied in the field of symbiotic culture of facultative anaerobic Streptococcus faecalis and yeast, can solve problems such as pH drop and difficult cultivation of Streptococcus faecalis, achieve good effects, reduce fermentation costs and Time, the effect of increasing the number of viable bacteria

Active Publication Date: 2015-05-13
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because Streptococcus faecalis is facultatively anaerobic, it is difficult to cultivate. In addition, Streptococcus faecalis has a relatively strong acid-producing ability, and can produce lactic acid during its growth process. As the lactic acid content gradually increases, the pH value will drop.

Method used

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  • Method for symbiotic culture of streptococcus faecalis and saccharomycetes
  • Method for symbiotic culture of streptococcus faecalis and saccharomycetes
  • Method for symbiotic culture of streptococcus faecalis and saccharomycetes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 The relationship between the different inoculum sizes of mixed bacteria and the amount of cultured bacteria

[0016] (1) The formula of the medium is: every 1000ml medium contains 24g of peptone, 18g of glucose and 8g of yeast powder.

[0017] (2) Method: According to the inoculation amount of Saccharomyces cerevisiae seed liquid, it is 2%, 2.5%, 3%, 3.5% respectively; according to the inoculation amount of Streptococcus faecalis seed liquid, it is 0.5%, 1.0%, 1.5%, 2.0% respectively ; The initial pH is 7.0, first inoculate the Streptococcus faecalis seed solution into the medium and cultivate for 4 hours, then add the Saccharomyces cerevisiae seed solution, and cultivate for 20 hours at 30°C with the shaking speed of 180rpm. see results figure 1 .

[0018] Saccharomyces cerevisiae and Streptococcus faecalis belong to fungi and bacteria respectively. The size of a single yeast cell is much larger than a single Streptococcus faecalis cell, so the consump...

Embodiment 2

[0019] Example 2 The amount of mixed bacteria in the liquid culture of different initial pH

[0020] (1) The formula of the medium is: every 1000ml medium contains 24g of peptone, 18g of glucose and 8g of yeast powder.

[0021] (2) Method: The inoculation volumes of Saccharomyces cerevisiae and Streptococcus faecalis seed solution were 2.5% and 1.0% respectively; the initial pH was 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 respectively, and the seed solution of Streptococcus faecalis was first inoculated into After culturing in the culture medium for 4 hours, Saccharomyces cerevisiae seed liquid was added, and cultured at 30° C. with a shaking speed of 180 rpm for 20 hours. see results figure 2 with image 3 .

[0022] Saccharomyces cerevisiae is an aerobic bacteria. Streptococcus faecalis is a facultative anaerobic bacterium that figure 2 It can be seen that when the initial pH is 7.0, the number of live bacteria of Saccharomyces cerevisiae and Streptococcus faecalis is highe...

Embodiment 3

[0024] Example 3 The amount of cultured bacteria of Streptococcus faecalis and Saccharomyces cerevisiae in different ways

[0025] (1) The formula of the medium is: every 1000ml medium contains 24g of peptone, 18g of glucose and 8g of yeast powder.

[0026] (2) Method: The inoculum volume of Saccharomyces cerevisiae and Streptococcus faecalis seed solution was 2.5% and 1.0% respectively; the initial pH was 7.0, according to the feeding sequence shown in Table 1, at 30°C, the shaking speed was 180rpm, and cultivated for 20h. The results are shown in Table 1.

[0027]

[0028] It can be seen from Table 1 that Saccharomyces cerevisiae and Streptococcus faecalis were cultured symbiotically, and different inoculation sequences had a significant impact on the final culture effect of these two strains. Streptococcus faecalis is first inoculated into the liquid medium and then cultivated to its pre-log, middle and end-logarithmic stages, and then inoculated with Saccharomyce...

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Abstract

The invention relates to a method for symbiotic culture of streptococcus faecalis and saccharomycetes. The technical scheme is that the optimum formulation of a culture medium is 2.4 g% peptone, 1.2-2.4 g% glucose, and 0.5-1 g% yeast powder, and the initial PH value is 7.0; the cultivation method comprises the following steps: the inoculum sizes of saccharomycetes seed solution and streptococcus faecalis seed solution are 2.5% and 1.0% respectively, the streptococcus faecalis seed solution is firstly inoculated in the culture medium to be cultured to the early exponential phase (about 4 hours), then the saccharomycetes seed solution is added, and at the temperature of 30 DEG C, the oscillating rotational speed is 180 rpm, the cultivation time is 20 hours, and next streptococcus faecalis and saccharomycetes are subjected to enlarge cultivation in a 5L fermentation tank, wherein the cultivation condition is that the throughput is 2.4 Lpm, the stirring rate is 100 rpm, the tank temperature is 30 DEG C, the broth content is 60%, and the cultivation time is 24 hours. The method is low in cost and stable in action effect, improves the number of compound bacteria, and enhances the action effect of a streptococcus faecalis and saccharomycetes complex microbial inoculant.

Description

technical field [0001] The invention relates to a method for symbiotic cultivation of microorganisms, in particular to a method for symbiotic cultivation of facultative anaerobic streptococcus faecalis and yeast. Background technique [0002] As an excellent probiotic strain, Streptococcus faecalis is a microbial preparation made through a series of processes. After being directly fed to livestock and poultry, it is conducive to improving the microbial structure in the gastrointestinal tract of livestock and poultry, and competitively repelling pathogenic microorganisms in the gastrointestinal tract. , maintain its micro-ecological balance, enhance the digestive function, promote the nutrient absorption and healthy development of livestock and poultry, improve the body's immunity, ensure that livestock and poultry give full play to the genetic advantages of growth, and indirectly increase production capacity. This has been widely used in livestock and poultry feed in many re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/18C12R1/46C12R1/865
Inventor 付保荣孙冶李铁民杜麟杨玉东刘宏生郭鑫
Owner LIAONING UNIVERSITY
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