Preparation method of temperature-sensitive hydrogel precursor

A temperature-sensitive hydrogel and precursor technology, applied in the field of hydrogel, can solve the problems of temperature-sensitive hydrogel material limitations, low mechanical strength, and rapid hydrogel erosion

Inactive Publication Date: 2019-07-02
长睿生物技术(成都)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, temperature-sensitive hydrogel materials have problems such as poor controllability of gelation temperature, low mechanical strength, rapid dissolution of the formed hydrogel, short hydrogel state stabilization time, and inability to biodegrade in vivo, making the temperature-sensitive hydrogel materials application is limited

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  • Preparation method of temperature-sensitive hydrogel precursor
  • Preparation method of temperature-sensitive hydrogel precursor
  • Preparation method of temperature-sensitive hydrogel precursor

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preparation example Construction

[0014] The embodiment of the present invention provides a method for preparing a temperature-sensitive hydrogel precursor, which includes:

[0015] S1. The biological material is fully dissolved under the condition that the molecule is non-electrically neutral to obtain a biological material solution.

[0016] Wherein, the biological material includes at least one of natural biological material and synthetic biological material. Natural biomaterials include starchy materials, cellulose-based materials, alginic acid, alginate, serum albumin, fibrinogen, hyaluronic acid, isinglass, chitosan, chitosan / disodium glycerophosphate mixture, At least one of thromboplastin, collagen, gelatin, herringin, keratins, and reduced keratin. Starch materials include starch and starch derivatives, and cellulose materials include cellulose and cellulose derivatives. The synthetic biological material includes at least one of polyamino acid, polyvinyl alcohol, polyethylene glycol, acrylic acid, a...

Embodiment 1

[0038] This embodiment provides a thermosensitive hydrogel precursor, and its preparation method is as follows:

[0039] S1. Stir and mix the isinglass and hydrochloric acid solution evenly at 8° C. to prepare a isinglass solution with a isinglass content of 1 wt%.

[0040] S2. Maintain stirring at -1°C, slowly add NaOH solution dropwise to the isinglass solution, and adjust the pH value of the isinglass solution to 5.1.

[0041] S3. Continue stirring at 1° C., and slowly add a pH value stabilizer to the above isinglass solution, the addition method is 2 wt % per minute, and the addition is completed in 50 minutes.

[0042] The specific ion / molecule pair contained in this pH stabilizer is Tris-H + , after the addition, Tris-H in the solution + The concentration is 10M. In order to precisely control the pH value of the solution, NaOH solution was further added dropwise to ensure that the pH range of the solution was 5.5±1.

Embodiment 2

[0044] This embodiment provides a thermosensitive hydrogel precursor, and its preparation method is as follows:

[0045] S1. Stir and mix chitosan, sodium alginate and ammonia solution evenly to form a solution with chitosan content of 10wt% and sodium alginate content of 0.01wt%.

[0046]S2. Maintain stirring at 4° C., slowly add citric acid solution dropwise to the above solution, and adjust the pH value of the solution to 8.3.

[0047] S3. Continue to stir at -2°C, and slowly add a pH value stabilizer to the above solution, the addition method is 5 wt% per minute, and the addition is completed in 30 minutes.

[0048] The specific ion / molecule pair contained in this pH stabilizer is NH 4 + -NH 3 and HPO 4 2- -H + - Triethylamine, after addition, NH in solution 4 + -NH 3 The concentration is 5M, HPO in solution 4 2- -H + - The concentration of triethylamine is 0.01M. The pH range of the solution was 7.8±1.

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Abstract

The invention relates to a preparation method of a temperature-sensitive hydrogel precursor, which relates to the field of hydrogels, wherein the biological material is sufficiently dissolved under the condition that the molecule is not electrically neutral to obtain a solution of the biological material. By dissolving in non-electric neutral condition, the biological material can be dissolved more fully, and a stable and uniform biological material solution can be obtained. At low temperature, that biological material solution is adjusted that the molecule is electrically neutral. The low temperature environment ensures that the solution of biological material can be kept in the state of solution all the time in the process of adjusting to obtain the electrical neutrality, and the precipitation of biomaterials and destroying the stability of the solution are avoided. Finally, the pH value stabilizer is added to control the pH range of the temperature-sensitive hydrogel precursor accurately, so that the temperature-sensitive hydrogel precursor could maintain the stability of the pH range during the phase transition process, thus realizing the continuous, rapid, reversible and stable phase transition process of the temperature-sensitive hydrogel precursor.

Description

technical field [0001] The invention relates to the field of hydrogels, in particular to a method for preparing a temperature-sensitive hydrogel precursor. Background technique [0002] Thermosensitive hydrogel materials are a class of smart materials that are sensitive to temperature changes. They can undergo phase transitions with the use conditions or temperature changes at the site of use. Non-chemically cross-linked hydrogels are formed from aqueous solutions, and the formation is often hydrogen bonds after temperature changes. Or the hydrophobic interaction is caused by the subsequent change, which eventually leads to a change in the physical state of the aqueous solution, that is, when the ambient temperature is lower than the lower critical solution temperature (Lower Critical Solution Temperature, LCST), it is in a liquid state, and when it is higher than the LCST, it is in a semi-solid state. gel state. After in situ injection into the human body, the material can...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08L5/04C08L89/00C08L29/04C08L33/26C08L3/02C08L1/28C08L71/02C08J3/075
CPCC08J3/075C08J2305/08C08J2405/04C08J2489/00C08J2329/04C08J2333/26C08J2403/02C08J2389/00C08J2401/28C08J2371/02
Inventor 杜明春邓飞沈健
Owner 长睿生物技术(成都)有限公司
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