Serum-free protein-free culture medium

A protein-free medium and serum-free technology, applied in the field of culture medium, can solve the problem of high price of serum-free medium, and achieve the effects of low production cost, stable structure and improved quality

Inactive Publication Date: 2019-03-29
镇江桥美科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: for the problems referred to above, one of purpose of the present invention is to provide a kind of serum-free protein-free culture medium, to solve the high technical problem of the price of serum-free culture medium

Method used

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  • Serum-free protein-free culture medium
  • Serum-free protein-free culture medium
  • Serum-free protein-free culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0018] A serum-free and protein-free medium, including the following components: the content of amino acids is: L-alanine 40mg / L, L-arginine hydrochloride 100mg / L, L-asparagine 500mg / L, L-glutamic acid 100mg / L, L-aspartic acid 60mg / L, L-cystine dihydrochloride 200mg / L, L-glutamine 1000mg / L, L-glycine 60mg / L, L-isoleucine amino acid 200mg / L, L-histidine hydrochloride 80mg / L, L-leucine 200mg / L, L-lysine 300mg / L, L-methionine 100mg / L, L-phenylalanine 100mg / L L, L-proline 80mg / L, L-serine 80mg / L, L-threonine 300mg / L, L-tryptophan 40mg / L, L-tyrosine disodium salt 300mg / L, L- Valine 400mg / L, biotin 0.2mg / L, calcium pantothenate 10mg / L, choline chloride 10mg / L, folic acid 10mg / L, inositol 20mg / L, nicotinamide 10mg / L, riboflavin 1mg / L L, pyridoxal hydrochloride 10mg / L, thiamine hydrochloride 10mg / L, vitamin B 12 0.025mg / L, anhydrous calcium chloride 500mg / L, potassium chloride 800mg / L, potassium nitrate 0.2mg / L, magnesium sulfate 300mg / L, sodium chloride 8000mg / L, sodium dihydrogen...

Embodiment 2

[0021] A serum-free protein-free medium, including the following components: L-alanine 5mg / L, L-arginine hydrochloride 10mg / L, L-asparagine 10mg / L, L-glutamic acid 10mg / L, L-aspartic acid 10mg / L, L-cystine dihydrochloride 50mg / L, L-glutamine 100mg / L, L-glycine 10mg / L, L-isoleucine 50mg / L, L-histidine hydrochloride 10mg / L, L-leucine 50mg / L, L-lysine 100mg / L, L-methionine 10mg / L, L-phenylalanine 40mg / L, L-proline Acid 20mg / L, L-serine 10mg / L, L-threonine 50mg / L, L-tryptophan 10mg / L, L-tyrosine disodium salt 50mg / L, L-valine 50mg / L , biotin 0.005mg / L, calcium pantothenate 1mg / L, choline chloride 1mg / L, folic acid 1mg / L, inositol 3mg / L, nicotinamide 1mg / L, riboflavin 0.1mg / L, pyridoxine hydrochloride Aldehyde 1mg / L, Thiamine Hydrochloride 1mg / L, Vitamin B 12 0.005mg / L, anhydrous calcium chloride 100mg / L, potassium chloride 200mg / L, potassium nitrate 0.01mg / L, magnesium sulfate 50mg / L, sodium chloride 2000mg / L, sodium dihydrogen phosphate 100mg / L, right Glucose 1000mg / L, HEPES...

Embodiment 3

[0024] A serum-free protein-free medium, including the following components: L-alanine 20mg / L, L-arginine hydrochloride 60mg / L, L-asparagine 300mg / L, L-glutamic acid 50mg / L, L-aspartic acid 30mg / L, L-cystine dihydrochloride 110mg / L, L-glutamine 600mg / L, L-glycine 40mg / L, L-isoleucine 120mg / L, L-histidine hydrochloride 40mg / L, L-leucine 130mg / L, L-lysine 210mg / L, L-methionine 60mg / L, L-phenylalanine 70mg / L, L-proline Acid 50mg / L, L-serine 40mg / L, L-threonine 170mg / L, L-tryptophan 25mg / L, L-tyrosine disodium salt 170mg / L, L-valine 220mg / L , biotin 0.105mg / L, calcium pantothenate 4mg / L, choline chloride 6mg / L, folic acid 6mg / L, inositol 13mg / L, nicotinamide 7mg / L, riboflavin 0.6mg / L, pyridoxine hydrochloride Aldehyde 6mg / L, thiamine hydrochloride 7mg / L, vitamin B 120.014mg / L, anhydrous chloride / 310mg / L, potassium chloride 520mg / L, potassium nitrate 0.12mg / L, magnesium sulfate 180mg / L, sodium chloride 4300mg / L, sodium dihydrogen phosphate 310mg / L, right Spinose 6000mg / L, HEPES...

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Abstract

The invention discloses a serum-free protein-free culture medium. The serum-free protein-free culture medium comprises amino acids, vitamins, inorganic salts, a buffering agent, phenolsulfonphthalein,dextrose, sodium pyruvate and sodium bicarbonate, wherein the amino acids include at least one of L-alanine, L-arginine monohydrochloride, L-asparagine, L-glutamic acid, L-aspartic acid, L-cystine dihydrochloride, L-glutamine, L-glycine, L-isoleucine, L-histidine hydrochloride, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine disodiumsalt and L-valine. The serum-free protein-free culture medium disclosed by the invention has the advantages that the structure is stable, the stable pH value range is maintained, and besides, the phenolsulfonphthalein can effectively display the pH value state of the culture medium; and the culture medium does not contain protein or serum completely, is low in production cost, is definite in chemical components, facilitates separation and purification of products, and can improve the quality of the products.

Description

technical field [0001] The invention relates to the technical field of medium, in particular to a serum-free and protein-free medium. Background technique [0002] Usually, the growth of animal cells depends on the presence of serum. In ordinary medium, most cells cannot proliferate without adding serum. At present, the better serum that is usually added to the serum medium is bovine serum. In addition to providing nutrients to cells, serum can also promote cells to start to synthesize DNA, which has a great effect on cell proliferation. Experiments have shown that serum provides growth factors necessary for cell proliferation in in vitro culture of cells. However, mammalian serum is expensive, and the high cost is not suitable for large-scale industrial production. Moreover, there are many problems with mammalian serum as a supplement. First, there is a problem with the source of serum. The serum comes from a specific area. Therefore, if a famine or epidemic disease occu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
CPCC12N5/0037C12N5/005C12N2500/60C12N2500/12C12N2500/14C12N2500/32C12N2500/34C12N2500/38C12N2501/999
Inventor 李忠马飞飞李宋敖徐逊
Owner 镇江桥美科技有限公司
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