Serum-free protein-free culture medium
A protein-free medium and serum-free technology, applied in the field of culture medium, can solve the problem of high price of serum-free medium, and achieve the effects of low production cost, stable structure and improved quality
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Embodiment 1
[0018] A serum-free and protein-free medium, including the following components: the content of amino acids is: L-alanine 40mg / L, L-arginine hydrochloride 100mg / L, L-asparagine 500mg / L, L-glutamic acid 100mg / L, L-aspartic acid 60mg / L, L-cystine dihydrochloride 200mg / L, L-glutamine 1000mg / L, L-glycine 60mg / L, L-isoleucine amino acid 200mg / L, L-histidine hydrochloride 80mg / L, L-leucine 200mg / L, L-lysine 300mg / L, L-methionine 100mg / L, L-phenylalanine 100mg / L L, L-proline 80mg / L, L-serine 80mg / L, L-threonine 300mg / L, L-tryptophan 40mg / L, L-tyrosine disodium salt 300mg / L, L- Valine 400mg / L, biotin 0.2mg / L, calcium pantothenate 10mg / L, choline chloride 10mg / L, folic acid 10mg / L, inositol 20mg / L, nicotinamide 10mg / L, riboflavin 1mg / L L, pyridoxal hydrochloride 10mg / L, thiamine hydrochloride 10mg / L, vitamin B 12 0.025mg / L, anhydrous calcium chloride 500mg / L, potassium chloride 800mg / L, potassium nitrate 0.2mg / L, magnesium sulfate 300mg / L, sodium chloride 8000mg / L, sodium dihydrogen...
Embodiment 2
[0021] A serum-free protein-free medium, including the following components: L-alanine 5mg / L, L-arginine hydrochloride 10mg / L, L-asparagine 10mg / L, L-glutamic acid 10mg / L, L-aspartic acid 10mg / L, L-cystine dihydrochloride 50mg / L, L-glutamine 100mg / L, L-glycine 10mg / L, L-isoleucine 50mg / L, L-histidine hydrochloride 10mg / L, L-leucine 50mg / L, L-lysine 100mg / L, L-methionine 10mg / L, L-phenylalanine 40mg / L, L-proline Acid 20mg / L, L-serine 10mg / L, L-threonine 50mg / L, L-tryptophan 10mg / L, L-tyrosine disodium salt 50mg / L, L-valine 50mg / L , biotin 0.005mg / L, calcium pantothenate 1mg / L, choline chloride 1mg / L, folic acid 1mg / L, inositol 3mg / L, nicotinamide 1mg / L, riboflavin 0.1mg / L, pyridoxine hydrochloride Aldehyde 1mg / L, Thiamine Hydrochloride 1mg / L, Vitamin B 12 0.005mg / L, anhydrous calcium chloride 100mg / L, potassium chloride 200mg / L, potassium nitrate 0.01mg / L, magnesium sulfate 50mg / L, sodium chloride 2000mg / L, sodium dihydrogen phosphate 100mg / L, right Glucose 1000mg / L, HEPES...
Embodiment 3
[0024] A serum-free protein-free medium, including the following components: L-alanine 20mg / L, L-arginine hydrochloride 60mg / L, L-asparagine 300mg / L, L-glutamic acid 50mg / L, L-aspartic acid 30mg / L, L-cystine dihydrochloride 110mg / L, L-glutamine 600mg / L, L-glycine 40mg / L, L-isoleucine 120mg / L, L-histidine hydrochloride 40mg / L, L-leucine 130mg / L, L-lysine 210mg / L, L-methionine 60mg / L, L-phenylalanine 70mg / L, L-proline Acid 50mg / L, L-serine 40mg / L, L-threonine 170mg / L, L-tryptophan 25mg / L, L-tyrosine disodium salt 170mg / L, L-valine 220mg / L , biotin 0.105mg / L, calcium pantothenate 4mg / L, choline chloride 6mg / L, folic acid 6mg / L, inositol 13mg / L, nicotinamide 7mg / L, riboflavin 0.6mg / L, pyridoxine hydrochloride Aldehyde 6mg / L, thiamine hydrochloride 7mg / L, vitamin B 120.014mg / L, anhydrous chloride / 310mg / L, potassium chloride 520mg / L, potassium nitrate 0.12mg / L, magnesium sulfate 180mg / L, sodium chloride 4300mg / L, sodium dihydrogen phosphate 310mg / L, right Spinose 6000mg / L, HEPES...
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