A method for producing a probiotic agent of live Lactobacillus bulgaricus

The technology of a live bacteria preparation and Lactobacillus is applied in the field of preparing probiotic feed additives by anaerobic fermentation of Lactobacillus bulgaricus in a liquid culture medium, and can solve the problems that the density of viable bacteria of the fermentation product is not high enough, the raw materials cannot be reused, and the production cost is high, Achieve the effect of inhibiting bacterial contamination, shortening fermentation cycle and reducing production cost

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The previous fermentation methods of Lactobacillus bulgaricus mainly have the following problems and deficiencies: (1) The anaerobic culture tank is used or the anaerobic environment is maintained by continuously feeding carbon dioxide or nitrogen. The raw materials cannot be reused, and the operation is more complicated and the cost is higher. High; (2) A large number of tryptone, yeast extract, beef extract and its enzymatic hydrolyzate are used as the main nutrients of the medium, and the production cost is high; (3) The post-treatment of liquid fermentation products requires high-speed refrigerated centrifuges Centrifugation and low-temperature freeze-drying, the process is complicated, the cost is high, and the yield is low; (4) The viable bacterial density of the fermentation product is not high enough

Method used

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Embodiment 1

[0036] The technical process for the preparation of embodiment 1 Lactobacillus bulgaricus live bacteria preparation comprises the following steps:

[0037] (1) Activation and value-added of strains:

[0038] The present invention adopts Lactobacillus bulgaricus cheese subspecies as the fermentation strain, the strain number: GIM1.189, which is purchased from the Guangdong Provincial Microbial Strain Preservation Center; a single colony of Lactobacillus bulgaricus is picked from the plate, and inoculated into a 20mL MRS culture container. Base (peptone 10.0 g, beef extract 10.0 g, yeast extract 5.0 g, diammonium hydrogen citrate 2.0 g, glucose 20.0 g, Tween 80 1.0 mL, sodium acetate 5.0 g, dipotassium hydrogen phosphate 2.0 g, magnesium sulfate 0.58 g , manganese sulfate 0.25 g, distilled water 1 000 mL, pH 6.2~6.6, high temperature and high pressure sterilization) test tubes, 37 ℃ deep static culture for 12~16 hours, the bacterial solution was inoculated with an inoculum ...

Embodiment 2

[0045] Embodiment 2 is different from above-mentioned embodiment 1:

[0046] (1) Culture medium formula: The raw materials and weight parts included in the medium are: wheat flour 50, corn flour 26, glucose 11, yeast extract powder 1.5, peptone 3, the raw materials should be calculated in grams, and the corresponding parts in mL of distilled water 900, dissolved, put into the fermenter for 100 ℃, high temperature and high pressure sterilization for 30 minutes, when the fermenter cools down to 35~45 ℃, add inorganic salt 1.3g / 1000mL base material, the composition of inorganic salt: MgSO 4· 7H 2 O 0.04%, MnSO 4 ·H 2 O 0.04%, in order to avoid the formation of precipitation, stir evenly during the addition process, then add liquid paraffin to a thickness of 1cm;

[0047] (2) Temperature-controlled fermentation: The inoculum amount of Lactobacillus bulgaricus liquid seeds is 3%, stirred evenly, the initial pH is 6.3, the pH is controlled at 6.0~6.8, the fermentation temperatu...

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Abstract

The present invention relates to a method for producing a probiotic agent of live Lactobacillus bulgaricus, belonging to the field of microbial technology and specially relates to a liquid anaerobic fermentation method for producing the probiotic agent of live Lactobacillus bulgaricus. The method comprises the steps of: employing the Lactobacillus bulgaricus as a fermentation strain which is inoculated on an optimized liquid fermentation medium, fermenting at a temperature of 37-42 DEG C for 12-14h, drawing off and determining the number of viable cells, adsorbing fermentation broth by using vegetable starch, drying, crushing, and packaging to prepare the probiotic agent of live Lactobacillus bulgaricus with stable viability. The method of the invention is advantageous in that: high density fermentation of the Lactobacillus bulgaricus can be completed by using an ordinary fermentation tank, with simple operation, stable effect and low energy consumption; and centrifuges, freeze-drying machines, etc. are not required, thus reducing production costs and improving production efficiency.

Description

[0001] technical field [0002] The invention relates to the field of microbial fermentation engineering, in particular to a method for preparing a probiotic feed additive through anaerobic fermentation of Lactobacillus bulgaricus on a liquid medium. [0003] Background technique [0004] Lactobacillus bulgaricus ( Lactobacillus bulgaricus ) is a bacterium named after the country, belonging to a subspecies of Lactobacillus germanus, a Gram-positive anaerobic bacterium. The cells are 2-9 μm long and 0.5-0.8 μm wide. A single cell is long rod-shaped or chain-shaped, with blunt rounded ends, non-motile, and does not produce spores. When cultured on milk, the colonies are colorless to off-white, usually cotton-like, 1-3 mm in diameter. The subspecies was first identified by the Bulgarian microbiologist Stamen Grigorov (Stamen Grigorov) in 1905 and named after his home country. In 1984, Weiss et al. confirmed it as German milk Subspecies of Bacillus. Lactobacillus bulgaricus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12R1/225
Inventor 李帅伟李本涛
Owner GUANGZHOU GLAM BIOTECH
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