Method for high-density fermentation of bacillus subtilis

A Bacillus subtilis, high-density fermentation technology, applied in the field of agricultural application microorganisms, can solve problems such as the increase of ammonia nitrogen and nitrite, affecting the size of fish and shrimp, etc.

Inactive Publication Date: 2015-01-21
LEADING GREEN BIOLOGICAL ZHENJIANG
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Problems solved by technology

[0002] At present, my country's aquaculture industry is mainly based on high-density farming. Therefore, farmers are faced with the problem of continuously increasing the amount of feed in order to pursue high yields, and on the other

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0013] (1) Inoculate the activated Bacillus subtilis into the culture medium with a 10% inoculation amount for shaker culture. The medium is: sucrose 20g / l, peptone 5g / l, sodium chloride 10g / l, pH = 7.0. The culture conditions are: 1200r / min, temperature 37 degrees, ventilation 1:1; culture for 24 hours;

[0014] (2) Crush coconut shells into 60-mesh powder, soak in tap water at a ratio of 1:1 for 12 hours;

[0015] (3) Mix the soaked coconut shell, bran, and soybean meal powder in a ratio of 1:3:2, make a solid medium, and sterilize at high temperature. The sterilization temperature is 121 degrees, and the sterilization time is 0.5 hours. , and then after cooling to 40 degrees, inoculate the Bacillus subtilis liquid in step 1 into a solid medium under aseptic conditions, mix well, and then carry out ventilation culture at a temperature of 34 degrees, and the culture time is 36 hours;

[0016] (4) Dry the solid spores cultivated in step 3 at a temperature of 60 degrees, and ...

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Abstract

The invention relates to a method for high-density fermentation of bacillus subtilis. A mixed fermentation mode of combining liquid fermentation with solid fermentation is adopted, meanwhile, coconut shell powder is added to a solid fermentation culture medium; and the coconut shell powder contains 36.51% of lignin, 53.06% of cellulose and 29.27% of pentosan; and therefore, proliferation of bacillus subtilis can be promoted.

Description

technical field [0001] The invention belongs to the technical field of agricultural application microorganisms. Background technique [0002] At present, my country's aquaculture industry is mainly based on high-density farming. Therefore, farmers are faced with the problem of continuously increasing the amount of feed in order to pursue high yields, and on the other hand, ammonia nitrogen in the water due to a large amount of residual bait and excessive discharge of farmed animal manure. And the contradiction between the effect of elevated nitrite on the individual size and yield of fish and shrimp. At present, the quality of agricultural Bacillus subtilis products in the market is uneven, and it is urgent to improve the fermentation level of Bacillus subtilis. Contents of the invention [0003] technical problem [0004] The object of the present invention is to provide "a method for high-density fermentation of Bacillus subtilis", which improves the bacteria content of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 孙义
Owner LEADING GREEN BIOLOGICAL ZHENJIANG
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