Method for cleanly and efficiently producing microorganism bactericide
A technology of microbial inoculum and production method, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of difficult product extraction and high energy consumption, and achieve the reduction of organic matter content, high nutrient utilization rate, and high product quality. high rate effect
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Embodiment 1
[0030] Fermentation medium is added with a composite carrier with a particle size of less than 10um to ferment Bacillus subtilis
[0031] The specific steps are:
[0032] (1) Lyophilized strains stored at 4°C or liquid strains stored at -80°C are activated. Streak Bacillus subtilis ACCC10619 on the prepared LB (peptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L) solid petri dish, and then place it at 37°C Incubate in a biochemical incubator for 18 hours.
[0033] (2) Preparation of first-level seed liquid. Pick a typical uniform single colony from the activated petri dish and inoculate it into the pre-prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 37℃, rotate at 150rpm, and cultivate for 18 hours . Obtain first-level seed liquid.
[0034] (3) Preparation of secondary seed liquid. First, sterilize the air filter, air pipe and fermentor of the fermentation system for making seed liquid with steam, and the...
Embodiment 2
[0042] Fermentation of Bacillus licheniformis by adding a composite carrier with a particle size of less than 50um to the fermentation medium
[0043] The specific steps are:
[0044] (1) Lyophilized strains stored at 4°C or liquid strains stored at -80°C are activated. Bacillus licheniformis CICC23584 was streaked on the LB solid petri dish prepared in advance, and then placed in a biochemical incubator set at 35°C for 24 hours.
[0045] (2) Preparation of primary seed liquid. Pick a typical uniform single colony from the activated petri dish and inoculate it into the pre-prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 32℃, rotate at 150rpm, and incubate for 22 hours . Obtain first-level seed liquid.
[0046] (3) Preparation of secondary seed liquid. First, sterilize the air filter, air pipe and fermentor of the fermentation system for making seed liquid with steam, and then add the pre-prepared seed culture med...
Embodiment 3
[0055] Fermentation of Pseudomonas stutzeri by adding zeolite powder with a particle size of less than 50um
[0056] The specific steps are:
[0057] (1) Lyophilized strains stored at 4°C or liquid strains stored at -80°C are activated. Pseudomonas stutzeri CICC23621 was streaked on the LB solid petri dish prepared in advance, and then placed in a biochemical incubator set at 30°C for 24 hours.
[0058] (2) Preparation of primary seed liquid. Pick a typical uniform single colony from the activated petri dish and inoculate it into the pre-prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 30℃, rotate at 150rpm, and cultivate for 22 hours . Obtain first-level seed liquid.
[0059] (3) Preparation of secondary seed liquid. First, sterilize the air filter, air pipe and fermentor of the fermentation system for making seed liquid with steam, and then add the pre-prepared seed culture medium (LB medium, and add defoamer at...
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