Method for cleanly and efficiently producing microorganism bactericide

A technology of microbial inoculum and production method, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of difficult product extraction and high energy consumption, and achieve the reduction of organic matter content, high nutrient utilization rate, and high product quality. high rate effect

Active Publication Date: 2011-10-12
HANGZHOU XIANGTIANGE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to improve the difficulty in extracting the products of the existing liquid fermentation process and the problems of high energy consumption; the purpose of the present invention is also to reduce the content of organic matter in the fermentation wastewater, reduce the treatment cost of the fermentation wastewater, or directly reuse the product after obtaining it In the fermentation production of the product, the fermentation wastewater is zero-discharged; the purpose of the present invention is to increase the utilization rate of the culture substrate and the bacterial content per unit volume of the fermentation liquid by adding mineral carriers; the purpose of the present invention is to obtain the product not only Live microbial cells, as well as metabolites such as bacteriocins and enzymes, improve the use value of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Fermentation medium is added with a composite carrier with a particle size of less than 10um to ferment Bacillus subtilis

[0031] The specific steps are:

[0032] (1) Lyophilized strains stored at 4°C or liquid strains stored at -80°C are activated. Streak Bacillus subtilis ACCC10619 on the prepared LB (peptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L) solid petri dish, and then place it at 37°C Incubate in a biochemical incubator for 18 hours.

[0033] (2) Preparation of first-level seed liquid. Pick a typical uniform single colony from the activated petri dish and inoculate it into the pre-prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 37℃, rotate at 150rpm, and cultivate for 18 hours . Obtain first-level seed liquid.

[0034] (3) Preparation of secondary seed liquid. First, sterilize the air filter, air pipe and fermentor of the fermentation system for making seed liquid with steam, and the...

Embodiment 2

[0042] Fermentation of Bacillus licheniformis by adding a composite carrier with a particle size of less than 50um to the fermentation medium

[0043] The specific steps are:

[0044] (1) Lyophilized strains stored at 4°C or liquid strains stored at -80°C are activated. Bacillus licheniformis CICC23584 was streaked on the LB solid petri dish prepared in advance, and then placed in a biochemical incubator set at 35°C for 24 hours.

[0045] (2) Preparation of primary seed liquid. Pick a typical uniform single colony from the activated petri dish and inoculate it into the pre-prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 32℃, rotate at 150rpm, and incubate for 22 hours . Obtain first-level seed liquid.

[0046] (3) Preparation of secondary seed liquid. First, sterilize the air filter, air pipe and fermentor of the fermentation system for making seed liquid with steam, and then add the pre-prepared seed culture med...

Embodiment 3

[0055] Fermentation of Pseudomonas stutzeri by adding zeolite powder with a particle size of less than 50um

[0056] The specific steps are:

[0057] (1) Lyophilized strains stored at 4°C or liquid strains stored at -80°C are activated. Pseudomonas stutzeri CICC23621 was streaked on the LB solid petri dish prepared in advance, and then placed in a biochemical incubator set at 30°C for 24 hours.

[0058] (2) Preparation of primary seed liquid. Pick a typical uniform single colony from the activated petri dish and inoculate it into the pre-prepared LB liquid sterile medium, then place it in a constant temperature shaking incubator, set the temperature at 30℃, rotate at 150rpm, and cultivate for 22 hours . Obtain first-level seed liquid.

[0059] (3) Preparation of secondary seed liquid. First, sterilize the air filter, air pipe and fermentor of the fermentation system for making seed liquid with steam, and then add the pre-prepared seed culture medium (LB medium, and add defoamer at...

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PUM

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Abstract

The invention relates to a method for cleanly and efficiently producing a microorganism bactericide, and the method comprises the following steps: adding adsorptive carriers in a liquid yeast culture medium, such as one or more of active carbon, zeolite, medical stone, sepiolite, active soil, diatomite, montmorillonite, attapulgite or all other artificial porous adsorptive materials, carrying outhigh-temperature sterilization together with a culture medium, cooling at a proper temperature, inserting bacteria, fermenting according to proper bacteria breeding conditions, wherein viable bacteria and metabolic product are adsorbed on the carriers after the fermentation is completed; and quickly settling after standing, and collecting a settlement, and drying or drying after pelletizing the settlement. A supernatant fluid after the fermentation product is collected and can be used as recycled water after being subjected to sterilization, or be used for preparing a culture medium for recycling.

Description

Technical field [0001] The invention relates to a clean and efficient production method of microbial agents, and belongs to the field of fermentation engineering. Background technique [0002] Microbial agents refer to living microorganisms and their metabolites obtained by one or more microbial strains through a series of fermentation and post-processing processes. The dosage form is liquid, powder or granule. The main fermentation methods are solid fermentation and liquid fermentation. The main purposes are: as medicines, feed additives, crop pesticides, for crop disease prevention and yield increase, ecological environment protection and pollution prevention. Among them, environmentally friendly microbial agents are widely used in the improvement of aquaculture water bodies, the treatment of domestic garbage, the harmless treatment of livestock and poultry manure, the denitrification and phosphorus removal of industrial and domestic sewage, and the degradation of COD. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N11/14C12N11/00
Inventor 黄海智邱晓力储卫华黄丽丽陈瑞
Owner HANGZHOU XIANGTIANGE BIO TECH
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