Stable and safe streptococcus thermophilus viable bacteria preparation and preparation method thereof

The technology of streptococcus thermophilus and viable bacteria preparation is applied in the field of preparing probiotic feed additives through anaerobic fermentation of streptococcus thermophilus, which can solve the problem that the density of viable bacteria in fermentation products is not high enough, the raw materials cannot be reused, and the cost is high and other problems, to achieve the effect of inhibiting bacterial pollution, shortening the fermentation cycle and low cost

Inactive Publication Date: 2013-11-27
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The previous fermentation methods of Streptococcus thermophilus mainly have the following problems and deficiencies: (1) The anaerobic culture tank is used or the anaerobic environment is maintained by continuously feeding carbon dioxide or nitrogen. The raw materials cannot be reused, the operation is complicated, and the cost Higher; (2) The post-processing of liquid fermentation products requires high-speed refrigerated centrifuges for centrifugation and low-temperature freeze-drying, the process is complicated, the cost is high, and the yield is low; (3) The viable bacteria density of fermentation products is not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 prepares the technical process of the stable Streptococcus thermophilus live bacteria preparation, comprises the following steps:

[0028] (1) Strain activation:

[0029] The present invention adopts the viable Streptococcus thermophilus as the fermentation strain, which is purchased from the China Common Microorganism Strain Preservation Management Center, and the preservation number is 1.6472. 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, Tween 801.0mL, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25 g, agar powder 15.0g, distilled water 1000mL, pH 6.2~6.6, high temperature and high pressure sterilization) draw a line on the solid slope, incubate at 37°C for about 16 hours, and generally activate the bacteria 1 to 3 times;

[0030] (2) Preparation of primary seeds:

[0031] Pick a loop of activated Streptococcus thermophilus and inoculate it into ...

Embodiment 2

[0039] Embodiment 2 is different from above-mentioned embodiment 1 in that:

[0040](1) Medium formula: yeast extract 7.5g, peptone 7.5g, glucose 10g, KH 2 PO 4 2g, tomato juice 100mL, Tween80 0.5mL, distilled water 900mL, pH6.8, stir evenly, sterilize under high temperature and high pressure at 121°C for 20min, when the fermenter cools down to 45°C, add inorganic salt 1.3g / 1000mL base material, inorganic Salt composition: MgSO 4 ·7H 2 O 0.04%, MnSO 4 ·H 2 O 0.04%, in order to avoid the formation of precipitation, stir evenly during the addition process, then add liquid paraffin to a thickness of 1cm;

[0041] (2) Temperature-controlled fermentation: The inoculum amount of Lactobacillus bulgaricus liquid seeds is 3%, stirred evenly, the initial pH is 6.6, the pH is controlled at 6.6-6.8, the fermentation temperature is controlled at 40-42°C, and the fermentation is 16 hours , put the tank, and carry out the colony counting of the Streptococcus thermophilus culture, and ...

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Abstract

The invention relates to a production method for a streptococcus thermophilus viable bacteria preparation, belonging to the field of microbial technology and particularly relating to a liquid anaerobic fermentation production method for a streptococcus thermophilus micro-ecological preparation. The production method comprises the following steps of taking the streptococcus thermophilus as the fermented strain, inoculating into an optimized liquid medium, cultivating for 16-20 hours at 40-42 DEG C, putting the fermentation liquid in a tank and measuring the viable bacteria quantity, adsorbing the fermentation liquid through vegetable starch and drying, after treatment by polyethylene glycol (PEG) 4000 and sodium polyacrylate, drying, smashing and packaging, and preparing the streptococcus thermophilus preparation with stable activity. The production method has the advantages of completing high density fermentation of the streptococcus thermophilus through a common fermentation tank, being simple in operation, having stable effect and low energy consumption, using no equipment such as a centrifugal machine and a freeze dryer and the like, lowering the production cost, and improving the production efficiency.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for preparing probiotic feed additives through anaerobic fermentation of Streptococcus thermophilus in a liquid medium. Background technique [0002] Streptococcus thermophilus Facultative anaerobic or slightly aerobic Gram-positive bacteria, catalase negative, round or oval, paired or chained, non-spore-forming, mostly immobile . Streptococcus thermophilus is a normal intestinal flora of healthy people, which can grow and reproduce in the human intestinal tract. It can directly supplement the normal physiological bacteria of the human body, adjust the balance of intestinal flora, inhibit and remove potentially harmful bacteria in the intestinal tract. [0003] Streptococcus thermophilus is considered a "generally recognized as safe (GRAS)" ingredient and is widely used in the production of some important fermented dairy products, including yogurt and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/00A23K1/14C12N1/20C12R1/46
Inventor 李帅伟李本涛
Owner GUANGZHOU GLAM BIOTECH
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