The invention provides a biomass glycol refining method. According to the method, a glycol crude product obtained by a biomass route is used as a raw material, 8th, 9th and10th group transition metal iron, cobalt, nickel, ruthenium, rhodium, palladium, iridium and platinum are uses as catalyst active components, one step catalytic hydrogenation is performed in a fixed bed reactor at 40-160 DEG C under the hydrogen pressure of 0.2-10MPa to realize efficient refining of the crude glycol, the aldehyde content of the refined glycol product is less than 3ug/ g, and the UV transmittance at 220nm, 275nm and 350nm are respectively more than 75%, 92% and 99%. The crude glycol raw material comes from renewable resources, and has the advantages of being environmental friendly, green and renewable. In addition, the process has the advantages of short reaction time, good catalyst stability, good glycol UV transmittance, high space-time yield and the like, and has potential application prospects.