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Method for synthesizing sucrose fatty acid ester

A technology for the synthesis of fatty acid sugar esters, which is applied in the field of enzymatic synthesis, can solve the problems of insoluble polar opposite substrate environment, polluting enzyme activity, etc., and achieve the goal of simplifying the purification process of sugar esters, improving efficiency, and increasing space-time yield Effect

Inactive Publication Date: 2015-05-06
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for synthesizing fatty acid sugar esters, aiming to solve the problem of inability to dissolve substrates with opposite polarities and the existence of environmental pollution and enzyme activity in the existing enzymatic synthesis of fatty acid sugar esters. low level problem

Method used

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  • Method for synthesizing sucrose fatty acid ester
  • Method for synthesizing sucrose fatty acid ester
  • Method for synthesizing sucrose fatty acid ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, optimize enzyme amount

[0047] Substrate molar ratio (VL / Glc, Glc: 0.3M) 1:1, water content 0.08% (concentrated for 24h), solvent volume 1mL, molecular sieve 100mg, reaction temperature 40°C, enzyme volume 20mg, 40mg, 60mg, 80mg , 100mg and 120mg, under 300rpm shaker, react for 24h.

[0048] The ionic liquid is pre-concentrated in a vacuum centrifugal dryer at 60°C for 24 hours, and the vinyl laurate is dried for more than 2 weeks through 4 A molecular sieves (the same below).

[0049] From figure 1 It can be seen that the amount of enzyme does not increase significantly after 100 mg. Considering the factors of conversion rate and cost, the amount of enzyme is selected to be 100 mg.

Embodiment 2

[0050] Embodiment 2, optimize reaction temperature

[0051] Substrate molar ratio (VL / Glc, where Glc is 0.3M) 1:1, solvent volume 1mL, water content 0.08% (concentrated for 24h), enzyme volume 100mg, reaction temperatures were 30°C, 40°C, 50°C, 60°C ℃ and 70 ℃, under the shaker at 300rpm, react for 24h.

[0052] From figure 2 It can be seen from the figure that the conversion rate increases gradually before the reaction temperature is 60°C, but decreases after 60°C, so the optimal reaction temperature is 60°C.

Embodiment 3

[0053] Embodiment 3, optimize mol ratio

[0054] The amount of anhydrous glucose is 0.054g (0.3M), the amount of solvent is 1mL, the water content is 0.08% (concentrated for 24h), the molecular sieve is 100mg, the enzyme amount is 100mg, and the concentrations of vinyl laurate are: 0.1, 0.3, 0.6, 0.9 and 1.2M (that is, the VL / Glc molar ratios are: 1:3, 1:1, 2:1, 3:1, and 4:1), the reaction temperature is 40°C, and the reaction is 24h under the shaker at 300rpm.

[0055] From image 3 It can be seen that when the molar ratio is 3:1, the conversion rate of glycolipid is the highest.

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Abstract

The invention discloses a method for synthesizing sucrose fatty acid ester. The method comprises the following steps: by taking an ionic liquid or a double solvent of the ionic liquid and an organic solvent as a reaction medium, adding fatty acid or fatty acid ester and saccharides with hydroxyl to the reaction medium at a mole ratio of (1:3)-(4:1), and mixing for 3-7 minutes; and then adding lipase, and reacting for 4-48 hours to prepare the sucrose fatty acid ester. According to the method, an environment-friendly high-efficiency reaction system for synthesizing sugar ester through an enzymic method is established by designing and optimizing a reaction condition in a pure ionic liquid system and a double-solvent system; especially, the solubility of a matrix and a product is enhanced through the ionic liquid and tertiary amyl alcohol in the double-solvent system, so that the space time yield of the sugar ester is enhanced, a purifying process of the sugar ester is simplified, and the efficiency is improved.

Description

technical field [0001] The invention relates to the field of enzymatic synthesis, in particular to a method for synthesizing fatty acid sugar esters. Background technique [0002] Fatty acid sugar ester is a non-ionic biosurfactant composed of sugar as the hydrophilic part and fatty acid as the hydrophobic part. These two components are natural renewable resources, so fatty acid sugar ester is biodegradable and biologically Compatibility and non-toxic, tasteless, non-irritating and other excellent characteristics, have been widely used in food, cosmetics and pharmaceutical industries. [0003] At present, the synthesis of sugar esters in industrial production mainly adopts chemical synthesis, but it has harsh reaction conditions, needs to be carried out under the conditions of high temperature and high pressure or strong acid, poor specificity, accompanied by the occurrence of side reactions and the generation of various forms of derivatives. serious pollution and other sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/02
CPCY02P20/54
Inventor 杨缜林晓生
Owner SHENZHEN UNIV
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