Comonas testosteroni with denitrification and dephosphorization functions and application thereof

A technology of Comamonas and testosterone, applied in the direction of bacteria, microbial-based methods, biological water/sewage treatment, etc., can solve the basic problems of single denitrification or phosphorus removal function research, and achieve slow value-added and growth rate Fast, high cell yield results

Active Publication Date: 2012-07-04
PEKING UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Both the theory of heterooxynitrification-aerobic denitrification biological denitrification and the theory of PAOs / DPB biological phosphorus removal are based on the study of a single denitrification or phosphorus removal function. So far, no research has shown that a microorganism has both different functions. Functions of Oxynitrification-Aerobic Denitrification in Nitrogen Removal and Phosphorus Removal

Method used

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  • Comonas testosteroni with denitrification and dephosphorization functions and application thereof
  • Comonas testosteroni with denitrification and dephosphorization functions and application thereof
  • Comonas testosteroni with denitrification and dephosphorization functions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Implementation Example 1: Phosphorus removal capacity determination of Comamonas testosteroni under aerobic conditions

[0033] Comamonas testosteroni strain (preserved on March 19, 2009 in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short), the preservation number is CGMCC №2963, the same below) was inoculated in 1L In the LB medium, to prevent the invasion of miscellaneous bacteria and maintain the growth vigor of the bacteria, carry out enrichment culture. The cultured bacterial solution was centrifuged, washed three times with sterile water, and the optical density (OD 600 ) is the bacterial suspension of 1-2.

[0034] Take 5mL of the above bacterial suspension and add two test media containing 120mL (each L contains 5g sodium succinate, 0.1911gNH 4 Cl, 0.044g KH 2 PO 4 , 0.2g CaCl 2 2H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 (0, pH7.0~7.5) Erlenmeyer flasks, sealed with 9 layers of gauze, and cultur...

Embodiment 2

[0036] Implementation Example 2: Determination of Optimal Phosphorus Removal Conditions for Comamonas testosteroni

[0037] Using the shaking flask experiment mode, according to the principle of orthogonal experiment, with pH value, shaker culture temperature, shaker speed and carbon-nitrogen ratio as the influencing factors, a total of 9 orthogonal experiments with 4 factors and 3 levels were established to determine Optimal phosphorus removal conditions for the strain Comamonas testosteroni. The medium used in the experiment was the same as in Example 1 except that the content of sodium succinate was changed as needed.

[0038] Orthogonal experimental design and results are shown in Table 1.

[0039] Table 1 Orthogonal experiment design and results

[0040]

[0041] Through the analysis of the results of the orthogonal experiment, it was found that the influence of four factors on the phosphorus removal effect of the strain was as follows: rotational speed>temperature>c...

Embodiment 3

[0042] Implementation example 3: Synchronous denitrification and dephosphorization ability determination of Comamonas testosteroni

[0043] Comamonas testosteroni strains were inoculated in 1 L of LB medium to prevent the invasion of miscellaneous bacteria and maintain the growth vigor of the bacteria for enrichment culture. The cultured bacterial solution was centrifuged, washed three times with sterile water, and the optical density (OD 600 ) is the bacterial suspension of 1-2.

[0044] Take 2.5mL of the above bacterial suspension and add to two test media containing 120mL (each L contains 5g sodium succinate, 0.1911g NH 4 Cl, 0.044g KH 2 PO 4 , 0.2g CaCl 2 2H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 (0, pH 7.0-7.2) Erlenmeyer flasks, sealed with 9 layers of gauze, and cultured in a shaker at 30° C. at 150 rpm. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. Take a...

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Abstract

The invention relates to application of Comamonas Testosteroni with hetetrophic nitrification-aerobic denitrification and dephosphorization functions to wastewater treatment. The strain can metabolize by taking organic carbon as a unique carbon source and ammonia nitrogen as a unique nitrogen source, and the ammonia nitrogen is directly converted into a gas product through a hetetrophic nitrification-aerobic denitrification effect to fulfill the denitrification aim; the strain also can take nitrate nitrogen as a unique nitrogen source and converts the nitrate nitrogen into a gas product through an aerobic denitrification effect; and the strain also can intake inorganic phosphorus under the aerobic condition and convert the inorganic phosphorus into own components to fulfill the aim of removing the phosphorus from sewage. When the strain is applied to wastewater treatment, the nitrogen and the phosphorus can be synchronously removed under the single aerobic condition, a problem that wastewater is subjected to anaerobic phosphate release, anoxic denitrification and aerobic nitrification phosphorus-uptake by stages in the traditional wastewater treatment is well solved, and the invention has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of Comamonas testosteroni and its application in wastewater treatment. The strain has the function of heterotrophic nitrification-aerobic denitrification, and can simultaneously complete the removal of phosphorus-containing pollutants in sewage during the process of simultaneous nitrification and denitrification denitrification, thereby achieving the purpose of synchronous biological denitrification and phosphorus removal of domestic sewage. Background technique [0002] With the progress of the society and the development of the economy, the eutrophication pollution of various water bodies in our country is becoming more and more serious, and the problem of blue-green algae red tides occurs frequently, which has caused huge damage to the safety of water bodies and human health. The excess of nutrients such as nitrogen and phosphorus is the root cause of eutrophication in water bodies. When the concentration of N in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/01
Inventor 倪晋仁陈倩付东康邹仕强
Owner PEKING UNIV
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