The invention provides an integrated whole autotrophic nitrogen removal device and belongs to the technical field of biological nitrogen removal of wastewater. The device comprises a reaction device body, wherein a granular sludge chamber and an anaerobic sludge-biomembrane chamber are arranged in the body, a water feeding and distributing device, an aeration pipe and a partition board are sequentially arranged in the granular sludge chamber from bottom to top, a filler suspending layer, a filler stacking layer and a water outlet chamber are formed in the anaerobic sludge-biomembrane chamber from top to bottom, the anaerobic sludge-biomembrane chamber surrounds the outer side of the granular sludge chamber, the partition board is arranged in the middle of the granular sludge chamber, a gas-collecting hood is arranged at the upper part, a first gas outlet is formed in the gas-collecting hood, an annular overflow port communicated with the anaerobic sludge-biomembrane chamber is formed in the upper part of the inner wall of the granular sludge chamber, a buffer and a water distributor are connected to the upper part of the inner wall of the anaerobic sludge-biomembrane chamber, and asecond gas outlet is formed in the top. The device can be started in a short time, can effectively intercept sludge to create aerobic and anaerobic environments and improve the system biomass and diversity, occupies little floor space and is good in water treatment effect.