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Comamonas testosteroni and application thereof in synthesizing of 5-hydroxymethyl furoic acid

A technology of Comamonas, hydroxymethyl furoic acid, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of destroying the cell membrane and cell wall synthesis of microorganisms, slow HMF conversion, and poor HMF tolerance. , to achieve the effect of simplifying the separation and purification process, excellent reaction efficiency, and overcoming the unfriendly environment

Active Publication Date: 2017-11-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a major problem of microbial catalyzed HMF conversion is that HMF not only has a strong inhibitory effect on microorganisms, but also destroys the cell membrane and cell wall of microbial cells and inhibits the synthesis of RNA (Bioresour.Technol., 2000,74,25; Biochem.J .,2002,363,769; Biotechnol.Bioeng.,1999,65,24)
Therefore, most microorganisms usually have poor tolerance to HMF, and the conversion rate of catalytic HMF is slow.

Method used

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  • Comamonas testosteroni and application thereof in synthesizing of 5-hydroxymethyl furoic acid
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  • Comamonas testosteroni and application thereof in synthesizing of 5-hydroxymethyl furoic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Activation and cultivation of Comamonas testosteroni SC1588: Inoculate in 1.8% nutrient broth medium (peptone 10g / L, beef extract powder 3g / L, sodium chloride 5g / L, pH 7.2), at 30°C and 150r / min, activated for 12h; then, according to the inoculum size of 1%, into the fresh nutrient broth medium (1.8%), at 30 ℃ and 150r / min, cultured for 5h, collected bacterial cells.

Embodiment 2

[0032] Add 0.16mmol HMF (40mM) in 4mL phosphate buffered saline (200mM, pH 7.0), after mixing evenly, add the testosterone trichome activated and cultivated by Example 1 at a concentration of 30mg / mL (by cell wet weight) For Monomonas SC1588 cells, react at 35°C and 150r / min. The reaction was monitored by liquid chromatography ( image 3 ). After 11 h, the conversion of HMF was 100%, and the yield of HMFCA was 94%.

Embodiment 3

[0034]In 4mL phosphate buffer (200mM, pH 7.0), add 0.16mmol HMF (40mM), after mixing uniformly, add the testosterone plexus activated and cultivated by Example 1 at a concentration of 30mg / mL (by cell wet weight) For Monomonas SC1588 cells, react at 25°C and 150r / min. After 11 h, the conversion of HMF was 100%, and the yield of HMFCA was 91%.

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Abstract

The invention discloses Comamonas testosteroni and application thereof in the synthesizing of 5-hydroxymethyl furoic acid. The Comamonas testosteroni SC1588 is preserved in China Center for Type Culture Collection (CCTCC) on October 13th, 2016, and the preservation number of the Comamonas testosteroni SC1588 is CCTCC No. M2016562. The biological catalyst Comamonas testosteroni SC1588 has the advantages that the Comamonas testosteroni SC1588 has high tolerance to HMF, can catalyze a high-concentration substrate (160mM) to selectively oxidize to synthesize a target product, and yield reaches 98%; high substrate concentration, excellent reaction efficiency and good selectivity are achieved.

Description

technical field [0001] The invention belongs to the fields of biocatalysis and chemical engineering, and specifically relates to a strain of Comamonas testosteroni and its application in catalyzing the selective oxidation of 5-hydroxymethylfurfural to synthesize 5-hydroxymethylfurfural. Background technique [0002] In recent years, due to the increasing scarcity of fossil resources and the intensification of environmental pollution, the production of bio-based energy and platform compounds from renewable biomass has attracted widespread attention. 5-Hydroxymethylfurfural (5-hydroxymethylfurfural, HMF) is listed as a "Top 10+4" platform compound by the US Department of Energy (Green Chem., 2010, 12, 539). This bio-based platform compound can be prepared by dehydration of carbohydrates. HMF molecules have multiple active groups such as hydroxyl groups, aldehyde groups and carbon-carbon double bonds, which can be easily converted into various useful intermediates. For example...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/01
CPCC12P17/04C12R2001/01C12N1/205C12N1/20
Inventor 李宁张雪莹宗敏华
Owner SOUTH CHINA UNIV OF TECH
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