Microbial agent capable of rapidly decolorizing aniline blue as well as preparation method and application of microbial agent
A microbial agent, aniline blue technology, applied in the field of microbial engineering, can solve the problems of incomplete removal, slow reaction speed, poor treatment effect, etc., achieve good decolorization effect, low cost, and reduce the effect of chemical reagents
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Embodiment 1
[0019] The preparation of embodiment 1 microbial bacterial agent
[0020] Include the following steps:
[0021] (1) Comamonas testosteroni CT1 (Comamonas testosteroni ATCC11996), KF1 (Comamonas testosteroni ATCC49202), Acinetobacter calcoaceticus LM1 (Acinetobacter calcoaceticus), Pseudomonas stutzeri JP1 (Pseudomonas sp.) from -80 Take it out of the refrigerator at ℃, thaw at room temperature, add LB high-salt medium, and culture in shake flask at 37°C and 160r / min overnight to complete the activation of bacteria;
[0022] (2) Subculture the above-mentioned activated strain with LB high-salt medium, and adjust the OD of the bacteria 600 = 1.0, the same amount is prepared as a mixed bacterial solution; the mixed bacterial solution is concentrated at low temperature, the concentration volume is 4:1, the concentration temperature is -18°C, and the number of viable bacteria in the bacterial agent is obtained by counting method is about 2.85×10 12 CFU / g;
[0023] The LB high-sa...
Embodiment 2
[0024] The preparation of embodiment 2 microbial bacterial agents
[0025] Include the following steps:
[0026] (1) The same amount of Comamonas testosteroni CT1 (Comamonas testosteroni ATCC11996), KF1 (Comamonas testosteroni ATCC49202), Acinetobacter calcium acetate LM1 (Acinetobacter calcoaceticus), Pseudomonas stutzeri JP1 (Pseudomonas sp.) were obtained from Take it out of the refrigerator at -80°C, thaw at room temperature, add LB high-salt medium, and culture overnight at 37°C and 180r / min in a shaker flask to complete the activation of the bacteria;
[0027] (2) Subculture the above-mentioned activated strain with LB high-salt medium, and adjust the OD of the bacteria 600 = 1.0, the same amount is prepared as a mixed bacterial solution; the mixed bacterial solution is concentrated at low temperature, the concentration volume is 5:1, the concentration temperature is -20°C, and the number of viable bacteria in the bacterial agent is obtained by counting method is about ...
Embodiment 3
[0029] The preparation of embodiment 3 microbial bacterial agents
[0030] Include the following steps:
[0031] (1) The same amount of Comamonas testosteroni CT1 (Comamonas testosteroni ATCC11996), KF1 (Comamonas testosteroni ATCC49202), Acinetobacter calcium acetate LM1 (Acinetobacter calcoaceticus), Pseudomonas stutzeri JP1 (Pseudomonas sp.) were obtained from Take it out of the refrigerator at -80°C, thaw at room temperature, add LB high-salt medium, and culture overnight at 37°C and 200r / min in a shaker flask to complete the activation of the bacteria;
[0032] (2) Subculture the above-mentioned activated strain with LB high-salt medium, and adjust the OD of the bacteria 600 = 1.0, the same amount is prepared as a mixed bacterial solution; the mixed bacterial solution is concentrated at low temperature, the concentration volume is 6:1, the concentration temperature is -19°C, and the number of viable bacteria in the bacterial agent is obtained by counting method is about ...
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