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Microbial deodorant and preparation method thereof

A deodorant and microbial technology, applied in the field of microbial deodorant and its preparation, can solve the problems of poor effect of decomposing garbage waste, restricting the application of microbial deodorant, and difficult acquisition of complex bacteria, etc., to reduce the amount of garbage and remove Bad smell, effect of promoting purification

Inactive Publication Date: 2011-11-30
黄小娃
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial deodorant production process is relatively complicated, complex bacteria are not easy to obtain, and the effect of decomposing garbage is not good, which limits the further application of microbial deodorant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The microbial deodorant described in the application of the present invention is a microbial flora composed of Lactobacillus plantarum, Bacillus subtilis, Saccharomyces cerevisiae and Rhodopseudomonas palustris, wherein, with 10 8 CFU / ml is the unit, the total number of viable bacteria is ≥10, Lactobacillus plantarum ≥3, Saccharomyces cerevisiae ≥3, Rhodopseudomonas palustris ≥2, Bacillus subtilis ≥2.

[0053] In addition, in the deodorant, the lead content is ≤8 mg / L, the arsenic content is ≤4 mg / L, and the fluorine content is ≤60 mg / L, and Salmonella and mold cannot be detected.

[0054] The deodorant has a packaging specification of 25kg / barrel or 200kg / barrel, a pH value of 3.5-4.0, and is non-toxic and has no side effects. The shelf life is one year, and the storage method is sealed at room temperature and in a cool place. It is a brown liquid.

Embodiment 2

[0056] 1. The microbial deodorant, wherein the culture medium for the total number of viable bacteria is as follows:

[0057] Beef extract: 10.0g Glucose: 10.0g Yeast extract: 3.0g

[0058] Peptone: 10.0g KH 2 PO 4 : 2.0g NaCl: 4.0g

[0059] Agar: 20.0g Distilled water: 1000ml PH value: 6.2-6.8

[0060] And sterilized at 121°C for 20 minutes.

[0061] 2. The culture medium for Lactobacillus plantarum is as follows:

[0062] Casein hydrolyzate: 10.0g Glucose: 20.0g Yeast extract: 5.0g

[0063] Sodium acetate: 25.0g Disodium citrate: 2.0g TWEEN80: 1.0g

[0064] K H 2 PO 4 3H 2 O: 6.0g MnSO 4 4H 2 O: 0.15g MgSO 4 7H 2 O: 0.58g

[0065] FeSO 4 7H 2 O: 0.03g Agar: 15.0g Distilled water: 1000ml

[0066] PH value: 5.4 and sterilized at 120°C for 20 minutes.

[0067] 3. The Bacillus subtilis culture medium is as follows:

[0068] Beef extract: 3.0g Peptone: 10.0g NaCl: 2.0g

[0069] Agar: 2.0g Distilled water: 1000ml PH value: 6.2-7.4

[0070] And sterilized at 12...

Embodiment 3

[0083] The application of the present invention also provides the preparation method of the microbial deodorant, which includes the following steps: through strain combination screening, the above-mentioned constituent strains and their proportion relationship are optimized, and then the production is carried out according to the following process flow:

[0084] Preservation of strains→slope strains→shake flask culture→seed tank culture→seeds→mixed fermentation culture→quality inspection and observation of reserved samples→subpackaging→storage or sales.

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Abstract

The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit) / ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.

Description

technical field [0001] The application of the present invention relates to a microbial deodorant and a preparation method thereof, belonging to the technical field of environmental protection. Background technique [0002] There are many kinds of malodorous substances, and different types of substances have different odorous groups in their molecular structures, so they have different odors and thresholds. Among the odorous substances that are more harmful to human health are mercaptans, ammonia, hydrogen sulfide, dimethyl sulfide, trimethylamine, formaldehyde, styrene, chromic acid and phenols. Odor pollution mainly harms the respiratory system, circulatory system, digestive system, endocrine system and nervous system of the human body, seriously affects people's mental state and reduces the quality of life. [0003] With the improvement of people's living standards in our country, the pollution of the environment by the emission of stench has been increasing, and the poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L9/01C12N1/16C12N1/20A61L101/52C12R1/125C12R1/225C12R1/38C12R1/865
Inventor 黄小娃
Owner 黄小娃
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