Formula and method for immobilizing tick sample for scanning electron microscopy
A fixing method and scanning electron microscope technology, applied in the preparation of test samples, etc., can solve problems such as poor penetration ability, and achieve the effect of less time consumption and uniform coating
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[0016] Example 1. Twelve male adult ticks were checked to see if there was any residual host tissue in the false head base, and if they were removed, they were removed with fine surgical forceps. Carefully wash the body surface of ticks with PBS buffer (pH=7.2), and then use ultrasonic cleaning for 60 minutes. Divide the cleaned ticks into four groups, three in each group, immerse the first and second groups of ticks in PBS buffer, lightly puncture 3 small holes under the 1st to 3rd basal segments of the ticks under the dissecting microscope, At room temperature, fix the treated tick samples with 1.5% glutaraldehyde fixative for 3 hours, 2.5% glutaraldehyde fixative for 4 hours, and 3% glutaraldehyde fixative for 5 hours. Soak the third and fourth groups of ticks in distilled water, lightly puncture 3 small holes below the 1-3 basal segments of the ticks under a dissecting microscope, and put them in 2.5% glutaraldehyde fixative solution (fixed with no new formula) Solution),...
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