32results about How to "Enhance in vitro proliferative ability" patented technology

The invention relates to a culture medium containing human umbilical cord mesenchymal stem cell exudates and a preparation method and applications thereof. An umbilical cord mesenchymal stem cell finite cell line is established to identify biological characteristics of the umbilical cord mesenchymal stem cell, and the preparation method for the human umbilical cord mesenchymal stem cell exudates is established to verify that the human umbilical cord mesenchymal stem cell (HUCMSC) exudates can promote cell regeneration, improve cell function and suppress cell apoptosis in vitro. A detection of enzyme-linked immunosorbent assay (ELISA) shows that human umbilical cord mesenchymal stem cell exudates contain abundant cell factors which can promote cell proliferation and suppress cell apoptosis, such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF).

The invention relates to the field of protein, in particular to bioactive polypeptide LPYPYYA as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide LPYPYYA is Leu-Pro-Tyr-Pro-Tyr-Tyr-Ala. In vitro immune function regulation experiments and in vivo anti-aging experiments prove that the polypeptide LPYPYYA has better immune regulation functionand anti-aging activity; on one hand, the bioactive polypeptide LPYPYYA can enhance the in vitro multiplication capacity of lymphocytes and macrophages, improve the capability of the body for resisting external pathogen infection and reduce the disease incidence of the body; on the other hand, the activity of an in vivo antiperoxidase system can be improved; the function of the body for resistingthe exogenous irritation is enhanced, so that the body aging, senility and sickness probability is reduced; very important significance is realized on developing food, health products and medicines with the immune regulation function and the anti-aging function.

The present invention relates to the field of proteins, in particular to a bioactive peptide KQSLPPGLAVKDLK and its preparation method and application. The amino acid sequence of the bioactive peptide KQSLPPGLAVKDLK is Lys‑Gln‑Ser‑Leu‑Pro‑Pro‑Pro‑Gly‑Leu‑Ala‑Val‑Lys ‑Asp‑Leu‑Lys. The in vitro immune function regulation experiment proved that the biologically active peptide KQSLPPGLAVKDLK has a good immune regulation function. The biologically active peptide KQSLPPGLAVKDLK of the present invention has the ability to enhance the in vitro proliferation of macrophages and lymphocytes, improve the ability of the body to resist external pathogen infection, reduce the incidence of the body, improve the quality of life, and is useful for the development of food and health products with immune regulation functions. and drugs are of great importance.

The invention relates to the field of bioengineering medical materials and discloses a method for building tissue engineering limbus corneae. The method comprises the following steps of putting fresh pig corneoscleral limbus matrix containing a lamina elastica anterior into a decellularized limbus corneae protecting liquid added with a detergent and nuclease for oscillating acellular treatment; putting decellularized limbus corneae into the decellularized limbus corneae protecting liquid for rinsing to obtain the decellularized limbus corneae; inoculating seed cells into the decellularized limbus corneae for amplification in vitro; obtaining the tissue engineering limbus corneae when the seed cells are amplified to 3-5 layers on the decellularized limbus corneae, wherein the decellularized limbus corneae protecting liquid is a PBS buffer solution containing 5-12g / L hyaluronic acid, 5-20g / L chondroitin sulfate, 3-10g / L dextran and 2.5-5mg / L L-tobramycin. Human limbus corneae can be replaced with the tissue engineering limbus corneae material.

Provided is a polypeptide, the amino acid sequence of which is SLPQ. The polypeptide is prepared by fermented skimmed milk by Lactobacillus helveticus. The polypeptide can enhance immunity of a body, so also provided are food, health products and medicines comprising the polypeptide.

The invention relates to the field of proteins, in particular to a biologically active peptide RLAFIAHPKLG and a preparation method and application thereof. The amino acid sequence of the biologically active peptide RAFIAHPKLG is Arg-Leu-Ala-Phe-Ile-Ala-His-Pro-Lys-Leu-Gly . Through in vitro immunomodulatory function experiments, it was found that the biologically active peptide RLAFIAHPKLG has a good immunomodulatory function. The bioactive peptide RLAFIAHPKLG of the present invention has the ability to enhance the in vitro proliferation ability of lymphocytes, can promote macrophages to secrete cytokines, improve the body's ability to resist infection by external pathogens, reduce the body morbidity rate, improve the quality of life, and has immunomodulatory effects on development Functional foods, health products and medicines are of great significance.

The present invention relates to the field of proteins, in particular to a bioactive peptide GGYGSGGGSGGYGSRRF and its preparation method and application. The amino acid sequence of the bioactive peptide GGYGSGGGSGGYGSRRF is Gly-Gly-Tyr-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly ‑Tyr‑Gly‑Ser‑Arg‑Arg‑Phe. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide GGYGSGGGSGGYGSRRF has a good immune regulation function. The biologically active peptide GGYGSGGGSGGYGSRRF of the present invention can significantly enhance the in vitro proliferation ability of macrophages, can effectively inhibit inflammation caused by oxidation, has a good immune regulation effect, can improve the body's ability to resist external pathogen infection, and reduce the body's morbidity , improving the quality of life is of great significance to the development of food, health products and medicines with immune regulation function.

The present invention relates to the field of proteins, in particular to a bioactive peptide IAHPKLGKRIR and its preparation method and application. The amino acid sequence of the bioactive peptide IAHPKLGKRIR is Ile‑Ala‑His‑Pro‑Lys‑Leu‑Gly‑Lys‑Arg‑Ile‑Arg . In vitro immune function regulation experiments proved that the biologically active peptide IAHPKLGKRIR has a good immune regulation function. The biologically active peptide IAHPKLGKRIR of the present invention can promote the proliferation ability of lymphocytes in vitro, improve the immunity of the human body, and at the same time have a significant promotion effect on the ability of macrophages to phagocytize neutral red in vitro, improve the body's ability to resist external pathogen infection, reduce the body's It is of great significance to develop food, health care products and medicines with immune regulation function and improve the quality of life.

The invention relates to a skin renewal method. According to the skin renewal method, hair follicle stem cells are used as seed cells and silica gel dressing is used as a new cell transplantation carrier. The skin renewal method comprises the following steps: 1) cultivating and amplifying hair follicle stem cells; 2) cultivating dermal cells; 3) mixing the hair follicle stem cells cultivated and amplified in the step 1) and the dermal cells cultivated in the step 2); 4) dropwise adding mixed cells obtained in the step 3) into silica gel dressing for incubation; and 5) transplanting the cells and the silica gel dressing used in the step 4) to a part required to be subjected to skin renewal. The method provided by the invention not only can renew functional skin in the treatment of large-area skin injuries, but also can provide a simple validation method for the multipotency test on hair follicle stem cells in scientific research activities.

The present invention relates to the field of proteins, in particular to a biologically active polypeptide FSPANKKLTPKKY and its preparation method and application. The amino acid sequence of the biologically active polypeptide FSPANKKLTPKKY is Phe-Ser-Pro-Ala-Asn-Lys-Lys-Leu-Thr-Pro-Lys ‑Lys‑Tyr. Through the in vitro immune function regulation experiment, it is verified that the polypeptide FSPANKKLTPKKY has a good immune regulation function. The biologically active peptide FSPANKKLTPKKY of the present invention can significantly enhance the in vitro proliferation ability of lymphocytes, and has the ability to increase the induction of macrophage nitric oxide under certain concentration conditions, improve the body's ability to resist external pathogen infection, and reduce the body's morbidity , improving the quality of life is of great significance to the development of food, health products and medicines with immune regulation function.

The present invention relates to the field of proteins, in particular to a bioactive peptide SAPRHGSLGFLPRK and its preparation method and application. The amino acid sequence of the bioactive peptide SAPRHGSLGFLPRK is Ser‑Ala‑Pro‑Arg‑His‑Gly‑Ser‑Leu‑Gly‑Phe‑Leu ‑Pro‑Arg‑Lys. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide SAPRHGSLGFLPRK has a good immune regulation function. The biologically active peptide SAPRHGSLGFLPRK of the present invention can significantly promote the in vitro proliferation ability of macrophages, and at the same time increase the ability to induce nitric oxide, thereby improving the body's ability to resist external pathogen infection, reducing the body's morbidity rate, and improving life expectancy. Quality is of great significance to the development of food, health care products and medicines with immune regulation function.