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A kind of biologically active peptide rlafiahpklg and its preparation method and application

A technology of biologically active peptides and sequences, applied in the field of protein, to achieve good anti-inflammatory activity, improved ability, and improved quality

Active Publication Date: 2022-07-15
SHANGHAI JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of 60S ribosomal protein L29 protein polypeptide fragments in the prior art

Method used

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  • A kind of biologically active peptide rlafiahpklg and its preparation method and application
  • A kind of biologically active peptide rlafiahpklg and its preparation method and application
  • A kind of biologically active peptide rlafiahpklg and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Artificial synthesis of bioactive peptide RLAFIAHPKLG

[0031] 1. Synthesis of Bioactive Peptides

[0032] Synthetic bioactive peptide RLAFIAHPKLG.

[0033] 2. Confirmation of Bioactive Peptides

[0034] 1) UPLC analysis

[0035] The UPLC conditions are as follows:

[0036] Instrument: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer

[0037] Column Specifications: BEH C18 Column

[0038] Flow rate: 0.4mL / min

[0039] Temperature: 50℃

[0040] UV detection wavelength: 210nm

[0041] Injection volume: 2μL

[0042] Gradient conditions: solution A: water containing 0.1% formic acid (v / v), solution B: acetonitrile containing 0.1% formic acid (v / v)

[0043] Time(min) %A %B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0044] 2) Mass spectrometry

[0045] The mass spectrometry conditio...

Embodiment 2

[0059] Example 2 Immunoactivity test of biologically active peptides

[0060] 1. In vitro lymphocyte proliferation ability test of bioactive peptide RLAFIAHPKLG (MTT method)

[0061] 1. Experimental materials and instruments:

[0062] Reagents and materials: experimental animals balb / c mice (male, 6-8 weeks old, Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University); mouse spleen lymphocyte-derived bioactive peptide RLAFIAHPKLG obtained in Example 1; mouse lymphocytes Cell extract (purchased from Soleobor Company); RPMI1640 medium (purchased from GIBCO Company); 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide ( MTT, purchased from Amresco Company); Concanavalin (ConA, purchased from Sigma Company); Bovine Serum Albumin (BSA, purchased from Genebase Company); Pepsin (purchased from Sigma Company); Pancreatin (Corolase PP, purchased from Sigma Company) from AB Company).

[0063] Equipment: LRH-250F biochemical incubator, Shanghai...

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Abstract

The invention relates to the field of proteins, in particular to a biologically active peptide RLAFIAHPKLG and a preparation method and application thereof. The amino acid sequence of the biologically active peptide RAFIAHPKLG is Arg-Leu-Ala-Phe-Ile-Ala-His-Pro-Lys-Leu-Gly . Through in vitro immunomodulatory function experiments, it was found that the biologically active peptide RLAFIAHPKLG has a good immunomodulatory function. The bioactive peptide RLAFIAHPKLG of the present invention has the ability to enhance the in vitro proliferation ability of lymphocytes, can promote macrophages to secrete cytokines, improve the body's ability to resist infection by external pathogens, reduce the body morbidity rate, improve the quality of life, and has immunomodulatory effects on development Functional foods, health products and medicines are of great significance.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide RLAFIAHPKLG and a preparation method and application thereof. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have been well proven, such as anticancer, blood pressure lowering, antibacterial, cholesterol lowering, antidiabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] At present, the research on bioactive peptides mostly focuses on food-derived peptides, and there are few studies and reports on non-food-derived peptides. And research results have confirmed that, compared with food-d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉李政郭婷婷栾媛媛占文静张伯宇谢雨佳王娟
Owner SHANGHAI JIAOTONG UNIV
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