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Preparation method and application of rhizoma polygonati polysaccharide

The technology of polysaccharide polysaccharide and Polygonatum polysaccharide is applied in the field of preparation of effective components of traditional Chinese medicine, which can solve the problem of low polysaccharide purity and achieve the effects of enhancing phagocytic function, enhancing in vitro proliferation ability and improving proliferation activity.

Pending Publication Date: 2018-06-15
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The polysaccharide content in Rhizoma Polygonatum is generally above 10%. Currently, the extraction and separation methods used to extract Polygonatum polysaccharide include hot water extraction, alcohol analysis separation, microwave-assisted extraction, ultrasonic extraction, etc., but the purity of the extracted polysaccharide is not high (<80%)

Method used

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  • Preparation method and application of rhizoma polygonati polysaccharide
  • Preparation method and application of rhizoma polygonati polysaccharide
  • Preparation method and application of rhizoma polygonati polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of Polygonatum polysaccharide, comprising the steps of:

[0029] (1) Wash the Rhizoma Polygonatum, soak it in water for 15 minutes, pile it on a clean table for 6 hours, cover it with a damp cloth, and spray an appropriate amount of water during this period to keep it in a moist state, so that the moisture outside the medicinal material can slowly penetrate into the inner tissue of the medicinal material, making the medicinal material Moisten until there is no dryness; then put it in a steamer and steam for 6 hours after the water is boiled, until the medicinal materials are yellow-black and soft, turn off the heat and continue to simmer for 6 hours until the appearance is brown-black and shiny, take it out of the pot, and let the sealwort dry outside and inside Moisturize, then cut into 3-5mm thick slices, and dry in a constant temperature oven at 70°C.

[0030] (2) Take the slices and grind them into powder, first use 95% ethanol ((V / V, the percen...

Embodiment 2

[0031] Embodiment 2 Polygonatum Polysaccharide Regulatory Effects on Cyclophosphamide-Induced Immunodeficiency Mice

[0032] 1. Experimental Animals and Reagents

[0033] Eight-week-old SPF male Kunming mice (22±2) g were purchased from the Hubei Provincial Center for Disease Control and Prevention, and the experiments began after the mice were adaptively fed for 3 days.

[0034] Polygonatum polysaccharide: prepared in Example 1, concanavalin A (ConA) and lipopolysaccharide (LPS) were purchased from Sigma-Adrich (St.Louis, Missouri, USA); IL-1β, IL-6 and TNF-α content determination ELISA The kit was purchased from Nanjing Jiancheng Institute of Biology.

[0035] PBS buffer: 0.01M, pH=7.4.

[0036] MTT solution: Add 250mg MTT into 50mL PBS buffer to dissolve completely, filter through a microporous membrane, and store in a -20°C refrigerator away from light.

[0037] 2. Method

[0038] 2.1 Animal grouping and model preparation methods

[0039] 50 Kunming mice were randomly...

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Abstract

The invention relates to the field of a preparation method and application of a traditional Chinese medicine effective component and in particular relates to a preparation method of rhizoma polygonatipolysaccharide and application of the rhizoma polygonati polysaccharide to the preparation of an immunological enhancement medicine. The rhizoma polygonati polysaccharide is obtained by processing raw rhizoma polygonati, extracting and separating; when an extraction method provided by the invention is adopted, the extraction rate of the rhizoma polygonati polysaccharide is high and the content oftotal polysaccharide is high; the proliferation activity of the rhizoma polygonati polysaccharide on normal mouse spleen cells is improved; cyclophosphamide induced spleen and thymus gland index of mice with low immunity also can be improved; the in-vitro proliferation capability of spleen and thymus gland cells under the stimulation of concanavalin A is enhanced; the phagocytic function of macrophage of the mice with the low immunity and the capability of secreting IL-6 and TNF-alpha are enhanced; the rhizoma polygonati polysaccharide has actual application value in the aspect of treatment of diseases including immunodeficiency diseases, tumors and the like.

Description

technical field [0001] The invention relates to a preparation method and application field of effective ingredients of traditional Chinese medicines, in particular to a preparation method of polygonatum polysaccharide and its application in preparation of immune-enhancing medicines. Background technique [0002] Polygonatum is the dried rhizome of Polygonatum kingianum Coll.et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua of Liliaceae plants. Sweet and flat in nature and flavor, it belongs to the spleen, lung, and kidney meridians, and has the effect of invigorating qi and strengthening the spleen. Polygonatum has edible, medicinal, ornamental, and cosmetic effects. It is often used for deficiency of spleen and stomach, fatigue, lack of stomach yin, dry mouth, lack of food, dry cough due to lung deficiency, labor cough and hemoptysis, insufficient essence and blood, soreness of the waist and knees, Premature graying of beard and hair, internal heat and que...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04A61P35/00
CPCA61K31/715C08B37/0003
Inventor 邓旭坤段欢吕晓宇
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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