57 results about "Concanavalin A" patented technology

The invention belongs to the technical field of gram-positive bacterium detection, and particularly relates to a nano-probe for detecting gram-positive bacteria and a preparation method, a detection method and application of the nano-probe for detecting the gram-positive bacteria, and the nano-probe for detecting the gram-positive bacteria comprises concanavalin A modified magnetic nanoparticles and vancomycin modified long afterglow nanoparticles, the magnetic nanoparticles comprise FeCl3, the long afterglow nanoparticles comprise ZnGa2O4: Cr, and the preparation method comprises a preparation method of the concanavalin A modified magnetic nanoparticles and a preparation method of the vancomycin modified long afterglow nanoparticles. The nano-probe provided by the invention has the functions of recognition, separation and enrichment, interference resistance and signal providing in the detection of gram-positive bacteria, and provides a new detection means for the recognition and detection of gram-positive bacteria.

The invention belongs to the technical field of nano-probes, and particularly relates to a nano-probe and a preparation method and application thereof.The nano-probe comprises a magnetic nano-material and a long afterglow nano-material, the magnetic nano-material comprises magnetic nano-particles modified with concanavalin A and is used for recognizing, capturing and separating bacteria, and the long afterglow nano-material is a long afterglow nano-material. The long-afterglow nano-material comprises green long-afterglow nano-particles of which the surfaces are modified with polymyxin B and red long-afterglow nano-particles of which the surfaces are modified with vancomycin, the green long-afterglow nanoparticles with the surfaces modified with polymyxin B and the red long-afterglow nanoparticles with the surfaces modified with vancomycin are respectively used for identifying anti-interference signal output, the nano-probe is applied to identification of gram-negative and positive bacteria, the anti-interference capability of detection can be improved, the whole process is rapid and simple, and the cost is low. And the cost is low, and the stability is high.

The present invention relates to: a recombinant polypeptide specifically binding to B cells; and a composition for preventing or treating B cell lymphoma, comprising same as an active ingredient. The present invention can significantly improve direct killing activity against tumor cells by conjugating concanavalin A, which is a ligand of MPZL1, to an antibody molecule that recognizes a B cell-specific antigen such as CD20. Therefore, unlike therapeutic antibodies having main mechanisms of antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and antibody-dependent cell-mediated phagocytosis (ADP), the recombinant polypeptide of the present invention does not depend on operating mechanisms of patient immune systems, and thus can be effectively used in an effective therapeutic composition even for cancer patients with reduced immune activity.

We offer multi-layered noodles that contain high-amylose wheat flour, have good shape retention and separation, and are less prone to becoming crumbly. [Solution] Multi-layer noodles having a laminated structure with layer B disposed between two layers A, wherein the content of high-amylose wheat flour in the total mass of cereal flours and starches in layer A is X% by mass, and the content of high-amylose wheat flour in the total mass of cereal flours and starches in layer B is Y% by mass, where X>Y and 5≦X≦100, and the high-amylose wheat flour is wheat flour with an amylose content of 40% by mass or more in total starch as analyzed by the Concanavalin A method.

The invention discloses a CD8 + T cell culture solution and application thereof. The cell culture solution comprises an induction culture solution and an amplification culture solution, wherein the amplification culture solution comprises a basic culture medium, and the basic culture medium contains ginsenoside with the final concentration of 20-100 [mu] g / mL, concanavalin A with the final concentration of 20-100 [mu] g / mL and IL-2 with the final concentration of 100-1000 U / mL; the induction culture solution is based on an amplification culture medium, and a CD3 antibody with the final concentration of 50-100 ng / mL and a CD28 antibody with the final concentration of 50-100 ng / mL are added. The ginsenoside component in the CD8 + T cell culture fluid can induce and stimulate PBMC to be differentiated into CD8 + T cells, and concanavalin A cooperates with ginsenoside to promote rapid amplification and growth of the CD8 + T cells; meanwhile, the cell culture method is simple to operate and low in cost, and has certain universality.

Provided are a small molecule-DNA interaction mapping sequencing method and kit. The method comprises: construction of transposase A coupled and modified by a small molecule compound and biotin; and adding a solution containing the transposase A and magnesium ions into a cell suspension bound to concanavalin A magnetic beads for incubation; after free transposase A is eluted, adding a solution containing transposase B and magnesium ions for continuous incubation, wherein the transposase B is formed by ligating a second adapter at a cleavage activity target site of the transposase; and enriching cleaved DNA fragments and carrying out amplification and sequencing so as to complete construction of a chromatin DNA binding map sequencing library.

The invention discloses a colorimetric-fluorescence dual-mode detection method for salmonella and escherichia coli, and belongs to the technical field of biological detection. According to the invention, concanavalin A (ConA) is coupled with magnetic nanoparticles (MNPs) to form MNPs (at) ConA as a capture probe by recognizing the characteristic of capturing bacteria by sugar residues, and in addition, an antibody (Ab) can specifically recognize bacteria and is coupled with quantum dots (QDs) to form QDs (at) Ab as a fluorescence signal probe. The invention finds that MNPs can catalyze 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) to generate blue oxTMB which can be observed by naked eyes; and the QDs have strong fluorescence and can also be observed by naked eyes, so that obvious distinguishing is realized in Sal and Esc detection. And the signal is linearly related to the target bacteria, so that the visibility is good. The practicability of the method is verified by detecting milk tea, milk and fruit juice samples, and the recovery rate is 93.5-106%. The feasibility of detecting Sal and Esc by the method is verified, and the method has important application potential in food safety monitoring.

The invention provides an application of a donepezil or gardenia jasminoides ellis-phellodendron amurense component in preparation of a cholinergic regulation-based immune liver injury resisting medicine, and belongs to the technical field of biological medicines. The pharmaceutical composition for resisting immunological liver injury is a donepezil component or a gardenia jasminoides ellis-phellodendron amurense component. The invention solves the problems of single action target and incomplete regulation and control mechanism of the existing immunological liver injury treatment medicine, provides a treatment scheme with anti-inflammatory, anti-oxidation, liver cell apoptosis inhibition and cholinergic system regulation and control functions, explores a brand new medical application of donepezil, verifies the liver protection effect of donepezil in immunological liver injury, and provides a new application of donepezil in immunological liver injury. The application limitation is broken through; the treatment effect of the Jashima component in an immune liver injury model induced by concanavalin A is defined, a dual regulation mechanism of inhibiting AChE expression and increasing alpha7 nAChR expression is disclosed, and the application scene is expanded; a multi-dimensional regulation and control network of cholinergic anti-inflammatory pathway-NF-kappa B inflammatory pathway-hepatocyte apoptosis is constructed, and a more efficient and comprehensive treatment strategy is provided for immunological liver injury.

To provide high amylose wheat flour having excellent properties as a raw material for bakery food products.SOLUTION: Wheat flour for bakery food products has an amylose content in total starch of 40% or more as analyzed by the concanavalin A method, and a gel syneresis rate of 5.8% to 8.5%.SELECTED DRAWING: None

The invention discloses an electrochemical sensor for glycoprotein detection and a glycoprotein detection method. The method comprises the following steps: anchoring an aptamer-protein conjugate on the surface of a gold electrode, and then assembling an anti-fouling peptide around the aptamer-protein conjugate to form an imprinted self-assembled monolayer film. And removing the combined protein through an acid solution to form a biocompatible imprinting cavity capable of being used for recombining a target object. The imprinted cavity can eliminate non-specific adsorption and enhance targeted binding efficiency. The sensor can be used for directly detecting carcino-embryonic antigens with the concentration as low as 0.1 ng / mL through an electrochemical impedance spectroscopy method. Besides, homodimer concanavalin A (ConA) is used as a recognition element and a cross-linking agent, homodimer glucose oxidase (GOx) is assembled on the surface of the electrode in situ, a protein network can be formed, and enzyme catalysis signal amplification detection is achieved. Through in-situ formation of a ConA-GOx assembly, the sensitivity is improved by 100 times.

The application discloses an AChE@AuNPs immobilized enzyme paper chip and a preparation method and application thereof, and belongs to the technical field of pesticide detection. The preparation method of the AChE@AuNPs immobilized enzyme paper chip comprises the following steps: adding HAuCl4 solution dropwise on a paper sheet, standing, then adding a reducing agent solution to react, then adding a surfactant solution and a buffer solution, and obtaining a paper chip with in-situ generated nanogold after reaction; adding a concanavalin A solution dropwise on the paper chip with in-situ generated nanogold, reacting, then adding a bovine serum albumin solution to react, finally adding an AChE solution, and obtaining an AChE@AuNPs immobilized enzyme system after reaction; and the AChE@AuNPs immobilized enzyme system and a detection area constitute the AChE@AuNPs immobilized enzyme paper chip. The AChE@AuNPs immobilized enzyme paper chip has the advantages of low preparation cost, portability, rapid detection, clear results and the like.

The application discloses an air bacterial electrostatic collection and real-time monitoring system and method integrated with a microfluidic portable sensor, and belongs to the technical field of environmental monitoring, and comprises an aerosol sampling module, a microfluidic processing module, an electrochemical sensing module and a portable constant potential instrument module. Bacteria aerosols in the ambient air are charged by corona discharge to realize efficient enrichment and collection; the enriched liquid sample is sent into the microfluidic processing module and flows through a sensing electrode modified with CuFeCoPBA@MoS2 nano composite material, polydopamine and concanavalin A. Target bacteria are combined with the concanavalin A on the surface of the electrode in a broad-spectrum specific manner, the interface electron transfer is hindered, the portable constant potential instrument collects the attenuation change of differential pulse voltammetry electrochemical signals, and rapid quantitative detection of the concentration of air bacteria is completed. The application integrates efficient air aerosol collection and portable biosensing, and can realize one-step on-site capture and detection of air pathogens.

Insults to vital organs have serious and even life-threatening consequences. Organ insults have different etiologies and typically include drugs, toxins and ischemic insults. Acyl-CoA binding protein (ACBP), also known as diazepam-binding inhibitor (DBI), is an extracellular feedback regulator of autophagy. Here, the inventors report that injection of a monoclonal antibody neutralizing ACBP / DBI (α-DBI) protects the murine liver against ischemia / reperfusion damage, acute intoxication by acetaminophen and concanavalin A, as well as against liver fibrosis induced by bile duct ligation or carbon tetrachloride. Of note, the results support the contention that α-DBI mediates broad organ-protective effects against multiple insults. Thus, the present invention relates to methods and pharmaceutical composition of protecting organs from injuries comprising neutralization of Acyl-CoA Binding Protein.

To provide a seasoning, sword bean miso, which contains concanavalin A (ConA), a useful component derived from sword beans and expected to improve immunity, and canavalin or its fermentation product, expected to prevent lifestyle-related diseases, and which can be deliciously and reasonably ingested in sufficient amounts. [Solution] By fermenting the legumes of the sword bean using koji mold as a raw material, sword bean miso is brewed, which is rich in useful ingredients such as concanavalin A (ConA) and canavalin, as well as free amino acids that have a delicious flavor and high nutritional value.

To provide instant noodles containing amylose wheat flour and excellent in friability and texture.SOLUTION: Instant noodles contain high amylose wheat flour, which has a damaged starch content of 4.0 to 7.0 mass% as measured by the AACC method 76-31, and which accounts for 30 mass% or more of the total mass of cereals and starches, where the high amylose wheat flour has an amylose content of 40 mass% or more in the total starch as analyzed by the concanavalin A method.SELECTED DRAWING: None

To provide dried noodles that contain high-amylose wheat flour and exhibit reduced powderiness in texture.SOLUTION: A method for producing dried noodles comprises drying raw noodles at 70°C or higher, the raw noodles containing 30 to 100 mass% of high-amylose wheat flour in the total mass of cereal flour and starch, the high-amylose wheat flour being a wheat flour having an amylose content of 40 mass% or more in total starch as analyzed by the Concanavalin A method.SELECTED DRAWING: None

To provide dried noodles that contain high-amylose wheat flour and exhibit good texture.SOLUTION: A method for producing dried noodles comprises drying raw noodles containing 30 mass% or more of high-amylose wheat flour in the total mass of cereal flour and starch, the high-amylose wheat flour being a wheat flour having an amylose content of 40 mass% or more in total starch as analyzed by the Concanavalin A method, and the high-amylose wheat flour having a damaged starch amount of 4.0 to 6.3 mass% as measured by the AACC Method 76-31.SELECTED DRAWING: None

The application belongs to the technical field of nano probes, and particularly relates to a nano probe and a preparation method and application thereof. The nano probe comprises magnetic nano materials and long afterglow nano materials. The magnetic nano materials comprise magnetic nano particles modified with concanavalin A, and are used for recognizing, capturing and separating bacteria. The long afterglow nano materials comprise green long afterglow nano particles modified with polymyxin B and red long afterglow nano particles modified with vancomycin. The green long afterglow nano particles modified with polymyxin B and the red long afterglow nano particles modified with vancomycin are respectively used for recognizing and outputting anti-interference signals. The nano probe can be applied to identifying gram-negative and gram-positive bacteria, can improve the anti-interference capability of detection, and has the advantages of rapidness, simplicity, low cost and high stability.

The invention discloses a method for specifically separating eukaryotic cells from prokaryotic cells in various environments. The invention provides a concanavalin A and magnetic bead complex. The concanavalin A and magnetic bead complex is a complex formed by connecting concanavalin A and magnetic beads, the invention also provides a method for separating eukaryotic cells and prokaryotic cells, which comprises the following steps: adding the concanavalin A and magnetic bead complex into a cell resuspension to be separated, and incubating; and separating the eukaryote cells adsorbed by the magnetic beads from the prokaryote cells not adsorbed by the magnetic beads by using a magnetic frame. The raw materials such as the streptavidin magnetic beads and the concanavalin A involved in the method are low in price and simple to prepare; magnetic separation is utilized, and operation is simple and fast; the separation effect is good.

The application provides a compound feed for improving the disease resistance of breeding ducks and a preparation process thereof, and belongs to the technical field of breeding feeds; the preparation process comprises the following steps: preparing modified resistant starch acetate; preparing a bifidobacterium composite microbial agent; preparing a composite nano carrier; preparing an extract composite additive; and preparing a compound feed; after the resistant starch acetate is activated by 1,1-carbonyl diimidazole and then a concanavalin A solution is added for reaction, the modified resistant starch acetate is obtained; after the bifidobacterium is loaded by using the modified resistant starch acetate, the bifidobacterium can be protected from damage by gastric acid and digestive enzymes, and then the number of bifidobacterium reaching the large intestine of the breeding duck is improved, which is beneficial to better inhibiting the reproduction of harmful bacteria in the large intestine of the breeding duck and improving the disease resistance of the breeding duck; in addition, after the resistant starch acetate is modified by using the concanavalin A, the loading rate of the resistant starch acetate to the bifidobacterium is improved.

The invention relates to an engineered exosome for targeting bladder cancer cells as well as a preparation method and application of the engineered exosome, and the engineered exosome is specific siRNA (small interfering Ribonucleic Acid) which is subjected to surface modification with concanavalin A and loaded with CASC11, and is marked as ConA-EXO-siCASC11. According to the invention, the engineered exosome is modified by ConA, so that the CASC11-siRNA is effectively delivered to the BLCA cells, the therapeutic effect of the CASC11-siRNA on the BLCA cis-platinum resistance is explored, an emerging therapeutic scheme is provided for the cis-platinum resistance BLCA, and the development of the field of precise treatment based on the engineered exosome is promoted.

Injury to vital organs has serious and even life-threatening consequences. Organ injury has a variety of etiologies, typically including drugs, toxins, and ischemic injury. Acyl-CoA binding protein (ACBP), also known as diazepam-binding inhibitor (DBI), is an extracellular feedback regulator of autophagy. Herein, we report that injection of a monoclonal antibody neutralizing ACBP / DBI (α-DBI) protects mouse livers from ischemia / reperfusion injury, acute intoxication with acetaminophen and concanavalin A, and hepatic fibrosis induced by bile duct ligation or carbon tetrachloride. Notably, the results support the contention that α-DBI mediates a broad organ protective effect against multiple insults.

The invention discloses a burkholderia gladioli aptamer and a screening method thereof, and belongs to the technical field of food safety. The DNA (deoxyribonucleic acid) sequence of the burkholderia gladioli aptamer is as shown in SEQ ID No: 1. According to the screening method, computer-aided design is utilized, aptamer candidate sequences highly specifically bound with bacterial membrane protein are rapidly screened from a large-scale nucleic acid sequence library through a molecular docking technology, and the aptamer with excellent affinity and specificity is finally obtained through in-vitro experimental verification and optimization. The method overcomes the defects that a traditional aptamer screening method is long in time consumption, high in cost, complex in operation and the like, and a new way is provided for rapidly and efficiently screening the burkholderia gladioli specific recognition aptamer. The obtained aptamer is combined with fluorescent nano-enzyme for the first time, forms a double-probe structure with concanavalin A (ConA)-loaded magnetic beads, can be used for constructing a burkholderia gladioli rapid detection system, and has a wide application prospect in the fields of food safety monitoring, pathogenic bacterium detection and the like.

The invention discloses preparation and application of a rotary splicing paper-based enzymolysis device, and belongs to the technical field of micro-fluidic paper chips. The method comprises the following steps: firstly, fixing nanogold on a micro-fluidic paper chip, and then assembling enzyme molecules on the surface of the nanogold modified by concanavalin A, thereby forming a directional enzyme bonding-nanogold mediated bioreactor, namely a micro-enzyme reactor. According to the micro-enzyme reactor, due to the high surface area of the nanoparticles and a directional immobilization strategy, the performance of the enzyme is expected to exceed that of a bioreactor prepared by a random bonding method. In addition, the enzyme is in butt joint with or separated from the color developing device in a rotary splicing manner, so that the operation convenience and the reusability of the enzyme are improved. The rotary splicing paper-based enzymolysis device designed by the invention is simple and convenient to operate and low in enzyme consumption, does not depend on a large-scale instrument, can be used for screening acetylcholin esterase inhibitors and tracing the activity of traditional Chinese medicinal materials, and provides a new device for drug screening.

The invention provides a liquid gate graphene transistor sensor with a grid modified by concanavalin. The liquid gate graphene transistor sensor is used for detecting glucose in real time. The liquid gate graphene transistor sensor comprises a substrate, the grid, a source and a drain, wherein the grid, the source and the drain are arranged on the substrate; a graphene channel is arranged between the source electrode and the drain electrode; and the surface of the grid is modified with concanavalin A biomolecules. The probe concanavalin is fixed on the surface of the grid electrode of the graphene transistor, the concanavalin can be combined with glucose in a solution with extremely high affinity, the conformation change of the concanavalin in the combination process causes the change of capacitance, the double-electric-layer interface characteristic between the transistor and the sample solution is changed, and the sensitivity of the transistor is improved. The current in the graphene channel is converted, and the glucose concentration is detected by detecting the change of the current in the channel. The channel current of the liquid gate graphene transistor sensor provided by the invention changes along with the change of glucose concentration, real-time detection is realized, the lowest detection limit of glucose can reach 10 <-19 > M, and the liquid gate graphene transistor sensor has very high sensitivity.

We offer semi-fresh noodles that don't dissolve easily when boiled and have a firm texture. [Solution] Semi-fresh noodles having a moisture content of more than 15% by mass and 28% by mass or less, wherein the raw material flour for the semi-fresh noodles contains 20 to 100% by mass of high-amylose wheat flour in the total mass of grain flour and starch, and the high-amylose wheat flour is wheat flour in which the amylose content in the total starch, as analyzed by concanavalin A method, is 40% by mass or more.

The invention discloses concanavalin A-like agglutinin / glucanase structural domain protein FvCLG and application thereof in improving the yield of fusarium venanii hypha protein, and belongs to the fields of genetic engineering technology and food biotechnology. Compared with a wild type strain, the fusarium venanii FG31 has the advantages that the FvCLG gene on a genome is deleted, ethanol is not generated in a fermentation tank for 72 hours, and the protein content is obviously increased (as high as 61.34%) and is increased by 22.41% compared with the wild type strain. Meanwhile, when the FG31 strain is fermented, the sugar consumption is obviously reduced, the conversion rate from glucose to biomass and the conversion rate from glucose to protein are respectively improved by 44.02% and 76.41%, the utilization rate of a substrate is obviously improved, the loss of a carbon source is reduced, and the production cost is reduced. The fusarium venanii can be used for further developing genetic engineering modification work to cultivate a fusarium venanii strain with high protein content.

The invention relates to the field of computational chemistry and enzyme engineering, in particular to directional immobilization nuclease P1 and a nuclease P1 directional affinity immobilization method based on computational aided design. According to the method, calculation and experiment means such as molecular dynamics simulation are combined for the first time, and accurate affinity immobilization is carried out on nuclease P1 through directional design. According to charge distribution and hydrophilic and hydrophobic areas of nuclease P1, spatial distribution of key residues of nuclease P1 is combined, dopamine and an electrified polymer are adopted as modifiers and co-deposited on the surface of the mesoporous resin carrier, and excellent adsorption capacity and immobilization effect are shown. Furthermore, through double optimization of glutaraldehyde crosslinking and concanavalin A modification, the binding efficiency and the catalytic activity stability of the nuclease P1 are remarkably improved. According to the method, the problem that nuclease P1 is poor in stability in a free state is solved, efficient directional affinity immobilization is achieved through a brand new molecular design and interface optimization technology, and a feasible technical path is provided for industrial-scale application.

The application belongs to the field of chemistry and nanomaterial science, and particularly relates to a kind of polyphenol-amino acid fluorescent carbon quantum dots and its preparation method and application.The preparation method of the polyphenol-amino acid fluorescent carbon quantum dots comprises: after mixing polyphenol, amino acid and alkaline solution, heating is carried out to obtain polyphenol-amino acid fluorescent carbon quantum dots.The polyphenol-amino acid fluorescent carbon quantum dots have excellent anti-inflammatory activity, and can significantly reduce the NO concentration of LPS (lipopolysaccharide) and ConA (concanavalin A) stimulated Raw264.7 cells.In addition, the polyphenol-amino acid fluorescent carbon quantum dots also have excellent blood-brain barrier crossing efficacy.

The invention provides a method for determining the content of starch in food containing maltodextrin, which comprises the following steps: grinding 2-5g of sample to be detected, putting into a centrifuge tube, adding 25ml of water, adding lectin with concanavalin A (Con A), centrifuging, and collecting precipitate A as amylopectin; taking the supernate, adding n-butyl alcohol, centrifuging, discarding the supernate, and collecting a precipitate B which is amylose; the interference of maltodextrin in starch detection in food containing maltodextrin is removed, and the influence of maltodextrin on the accuracy of starch content determination in food is reduced.