22 results about "Concanavalin A" patented technology

We offer multi-layered noodles that contain high-amylose wheat flour, have good shape retention and separation, and are less prone to becoming crumbly. [Solution] Multi-layer noodles having a laminated structure with layer B disposed between two layers A, wherein the content of high-amylose wheat flour in the total mass of cereal flours and starches in layer A is X% by mass, and the content of high-amylose wheat flour in the total mass of cereal flours and starches in layer B is Y% by mass, where X>Y and 5≦X≦100, and the high-amylose wheat flour is wheat flour with an amylose content of 40% by mass or more in total starch as analyzed by the Concanavalin A method.

The invention discloses a CD8 + T cell culture solution and application thereof. The cell culture solution comprises an induction culture solution and an amplification culture solution, wherein the amplification culture solution comprises a basic culture medium, and the basic culture medium contains ginsenoside with the final concentration of 20-100 [mu] g / mL, concanavalin A with the final concentration of 20-100 [mu] g / mL and IL-2 with the final concentration of 100-1000 U / mL; the induction culture solution is based on an amplification culture medium, and a CD3 antibody with the final concentration of 50-100 ng / mL and a CD28 antibody with the final concentration of 50-100 ng / mL are added. The ginsenoside component in the CD8 + T cell culture fluid can induce and stimulate PBMC to be differentiated into CD8 + T cells, and concanavalin A cooperates with ginsenoside to promote rapid amplification and growth of the CD8 + T cells; meanwhile, the cell culture method is simple to operate and low in cost, and has certain universality.

The invention provides an application of a donepezil or gardenia jasminoides ellis-phellodendron amurense component in preparation of a cholinergic regulation-based immune liver injury resisting medicine, and belongs to the technical field of biological medicines. The pharmaceutical composition for resisting immunological liver injury is a donepezil component or a gardenia jasminoides ellis-phellodendron amurense component. The invention solves the problems of single action target and incomplete regulation and control mechanism of the existing immunological liver injury treatment medicine, provides a treatment scheme with anti-inflammatory, anti-oxidation, liver cell apoptosis inhibition and cholinergic system regulation and control functions, explores a brand new medical application of donepezil, verifies the liver protection effect of donepezil in immunological liver injury, and provides a new application of donepezil in immunological liver injury. The application limitation is broken through; the treatment effect of the Jashima component in an immune liver injury model induced by concanavalin A is defined, a dual regulation mechanism of inhibiting AChE expression and increasing alpha7 nAChR expression is disclosed, and the application scene is expanded; a multi-dimensional regulation and control network of cholinergic anti-inflammatory pathway-NF-kappa B inflammatory pathway-hepatocyte apoptosis is constructed, and a more efficient and comprehensive treatment strategy is provided for immunological liver injury.

The invention discloses an electrochemical sensor for glycoprotein detection and a glycoprotein detection method. The method comprises the following steps: anchoring an aptamer-protein conjugate on the surface of a gold electrode, and then assembling an anti-fouling peptide around the aptamer-protein conjugate to form an imprinted self-assembled monolayer film. And removing the combined protein through an acid solution to form a biocompatible imprinting cavity capable of being used for recombining a target object. The imprinted cavity can eliminate non-specific adsorption and enhance targeted binding efficiency. The sensor can be used for directly detecting carcino-embryonic antigens with the concentration as low as 0.1 ng / mL through an electrochemical impedance spectroscopy method. Besides, homodimer concanavalin A (ConA) is used as a recognition element and a cross-linking agent, homodimer glucose oxidase (GOx) is assembled on the surface of the electrode in situ, a protein network can be formed, and enzyme catalysis signal amplification detection is achieved. Through in-situ formation of a ConA-GOx assembly, the sensitivity is improved by 100 times.

The application discloses an AChE@AuNPs immobilized enzyme paper chip and a preparation method and application thereof, and belongs to the technical field of pesticide detection. The preparation method of the AChE@AuNPs immobilized enzyme paper chip comprises the following steps: adding HAuCl4 solution dropwise on a paper sheet, standing, then adding a reducing agent solution to react, then adding a surfactant solution and a buffer solution, and obtaining a paper chip with in-situ generated nanogold after reaction; adding a concanavalin A solution dropwise on the paper chip with in-situ generated nanogold, reacting, then adding a bovine serum albumin solution to react, finally adding an AChE solution, and obtaining an AChE@AuNPs immobilized enzyme system after reaction; and the AChE@AuNPs immobilized enzyme system and a detection area constitute the AChE@AuNPs immobilized enzyme paper chip. The AChE@AuNPs immobilized enzyme paper chip has the advantages of low preparation cost, portability, rapid detection, clear results and the like.

The application discloses an air bacterial electrostatic collection and real-time monitoring system and method integrated with a microfluidic portable sensor, and belongs to the technical field of environmental monitoring, and comprises an aerosol sampling module, a microfluidic processing module, an electrochemical sensing module and a portable constant potential instrument module. Bacteria aerosols in the ambient air are charged by corona discharge to realize efficient enrichment and collection; the enriched liquid sample is sent into the microfluidic processing module and flows through a sensing electrode modified with CuFeCoPBA@MoS2 nano composite material, polydopamine and concanavalin A. Target bacteria are combined with the concanavalin A on the surface of the electrode in a broad-spectrum specific manner, the interface electron transfer is hindered, the portable constant potential instrument collects the attenuation change of differential pulse voltammetry electrochemical signals, and rapid quantitative detection of the concentration of air bacteria is completed. The application integrates efficient air aerosol collection and portable biosensing, and can realize one-step on-site capture and detection of air pathogens.

To provide a seasoning, sword bean miso, which contains concanavalin A (ConA), a useful component derived from sword beans and expected to improve immunity, and canavalin or its fermentation product, expected to prevent lifestyle-related diseases, and which can be deliciously and reasonably ingested in sufficient amounts. [Solution] By fermenting the legumes of the sword bean using koji mold as a raw material, sword bean miso is brewed, which is rich in useful ingredients such as concanavalin A (ConA) and canavalin, as well as free amino acids that have a delicious flavor and high nutritional value.

The application belongs to the technical field of nano probes, and particularly relates to a nano probe and a preparation method and application thereof. The nano probe comprises magnetic nano materials and long afterglow nano materials. The magnetic nano materials comprise magnetic nano particles modified with concanavalin A, and are used for recognizing, capturing and separating bacteria. The long afterglow nano materials comprise green long afterglow nano particles modified with polymyxin B and red long afterglow nano particles modified with vancomycin. The green long afterglow nano particles modified with polymyxin B and the red long afterglow nano particles modified with vancomycin are respectively used for recognizing and outputting anti-interference signals. The nano probe can be applied to identifying gram-negative and gram-positive bacteria, can improve the anti-interference capability of detection, and has the advantages of rapidness, simplicity, low cost and high stability.

The invention discloses a method for specifically separating eukaryotic cells from prokaryotic cells in various environments. The invention provides a concanavalin A and magnetic bead complex. The concanavalin A and magnetic bead complex is a complex formed by connecting concanavalin A and magnetic beads, the invention also provides a method for separating eukaryotic cells and prokaryotic cells, which comprises the following steps: adding the concanavalin A and magnetic bead complex into a cell resuspension to be separated, and incubating; and separating the eukaryote cells adsorbed by the magnetic beads from the prokaryote cells not adsorbed by the magnetic beads by using a magnetic frame. The raw materials such as the streptavidin magnetic beads and the concanavalin A involved in the method are low in price and simple to prepare; magnetic separation is utilized, and operation is simple and fast; the separation effect is good.

The application provides a compound feed for improving the disease resistance of breeding ducks and a preparation process thereof, and belongs to the technical field of breeding feeds; the preparation process comprises the following steps: preparing modified resistant starch acetate; preparing a bifidobacterium composite microbial agent; preparing a composite nano carrier; preparing an extract composite additive; and preparing a compound feed; after the resistant starch acetate is activated by 1,1-carbonyl diimidazole and then a concanavalin A solution is added for reaction, the modified resistant starch acetate is obtained; after the bifidobacterium is loaded by using the modified resistant starch acetate, the bifidobacterium can be protected from damage by gastric acid and digestive enzymes, and then the number of bifidobacterium reaching the large intestine of the breeding duck is improved, which is beneficial to better inhibiting the reproduction of harmful bacteria in the large intestine of the breeding duck and improving the disease resistance of the breeding duck; in addition, after the resistant starch acetate is modified by using the concanavalin A, the loading rate of the resistant starch acetate to the bifidobacterium is improved.

The invention relates to an engineered exosome for targeting bladder cancer cells as well as a preparation method and application of the engineered exosome, and the engineered exosome is specific siRNA (small interfering Ribonucleic Acid) which is subjected to surface modification with concanavalin A and loaded with CASC11, and is marked as ConA-EXO-siCASC11. According to the invention, the engineered exosome is modified by ConA, so that the CASC11-siRNA is effectively delivered to the BLCA cells, the therapeutic effect of the CASC11-siRNA on the BLCA cis-platinum resistance is explored, an emerging therapeutic scheme is provided for the cis-platinum resistance BLCA, and the development of the field of precise treatment based on the engineered exosome is promoted.

The invention discloses preparation and application of a rotary splicing paper-based enzymolysis device, and belongs to the technical field of micro-fluidic paper chips. The method comprises the following steps: firstly, fixing nanogold on a micro-fluidic paper chip, and then assembling enzyme molecules on the surface of the nanogold modified by concanavalin A, thereby forming a directional enzyme bonding-nanogold mediated bioreactor, namely a micro-enzyme reactor. According to the micro-enzyme reactor, due to the high surface area of the nanoparticles and a directional immobilization strategy, the performance of the enzyme is expected to exceed that of a bioreactor prepared by a random bonding method. In addition, the enzyme is in butt joint with or separated from the color developing device in a rotary splicing manner, so that the operation convenience and the reusability of the enzyme are improved. The rotary splicing paper-based enzymolysis device designed by the invention is simple and convenient to operate and low in enzyme consumption, does not depend on a large-scale instrument, can be used for screening acetylcholin esterase inhibitors and tracing the activity of traditional Chinese medicinal materials, and provides a new device for drug screening.

We offer semi-fresh noodles that don't dissolve easily when boiled and have a firm texture. [Solution] Semi-fresh noodles having a moisture content of more than 15% by mass and 28% by mass or less, wherein the raw material flour for the semi-fresh noodles contains 20 to 100% by mass of high-amylose wheat flour in the total mass of grain flour and starch, and the high-amylose wheat flour is wheat flour in which the amylose content in the total starch, as analyzed by concanavalin A method, is 40% by mass or more.

The invention discloses concanavalin A-like agglutinin / glucanase structural domain protein FvCLG and application thereof in improving the yield of fusarium venanii hypha protein, and belongs to the fields of genetic engineering technology and food biotechnology. Compared with a wild type strain, the fusarium venanii FG31 has the advantages that the FvCLG gene on a genome is deleted, ethanol is not generated in a fermentation tank for 72 hours, and the protein content is obviously increased (as high as 61.34%) and is increased by 22.41% compared with the wild type strain. Meanwhile, when the FG31 strain is fermented, the sugar consumption is obviously reduced, the conversion rate from glucose to biomass and the conversion rate from glucose to protein are respectively improved by 44.02% and 76.41%, the utilization rate of a substrate is obviously improved, the loss of a carbon source is reduced, and the production cost is reduced. The fusarium venanii can be used for further developing genetic engineering modification work to cultivate a fusarium venanii strain with high protein content.

The application belongs to the field of chemistry and nanomaterial science, and particularly relates to a kind of polyphenol-amino acid fluorescent carbon quantum dots and its preparation method and application.The preparation method of the polyphenol-amino acid fluorescent carbon quantum dots comprises: after mixing polyphenol, amino acid and alkaline solution, heating is carried out to obtain polyphenol-amino acid fluorescent carbon quantum dots.The polyphenol-amino acid fluorescent carbon quantum dots have excellent anti-inflammatory activity, and can significantly reduce the NO concentration of LPS (lipopolysaccharide) and ConA (concanavalin A) stimulated Raw264.7 cells.In addition, the polyphenol-amino acid fluorescent carbon quantum dots also have excellent blood-brain barrier crossing efficacy.

The invention discloses a magnetic hydrothermal carbon material reversely wrapped by an unmarked cell membrane as well as a preparation method and application of the magnetic hydrothermal carbon material, magnetic carbon nanospheres are prepared by a hydrothermal method, and then carboxyl groups on the surfaces of the magnetic carbon nanospheres and residual amino groups of lectin such as concanavalin A are subjected to amide condensation, so that lectinized hydrothermal magnetic carbon nanospheres are obtained. Finally, agglutination reaction can be carried out on agglutinin and carbohydrate chains on the outer surface of the cell membrane, so that the cell membrane is wrapped from inside to outside. The biomimetic material can screen drugs acting on the inner side of a cell membrane, does not need to label the cell membrane, can reduce damage to membrane protein, provides a new technical means for deeply exploring lead compounds in natural products, and promotes development of traditional Chinese medicine anti-osteoporosis drugs. According to the invention, two novel potential anti-osteoporosis components targeting the inner surface of an osteoblast cell membrane in herba epimedii are successfully found.

The invention discloses a preparation method for synthesizing a biological conjugate (ConA-MB) of methylene blue (MB) and concanavalin A (ConA) and application of the biological conjugate. According to the preparation route, methylene blue reacts with sarcosine to generate a compound II, and the compound II is coupled with ConA to form the ConA-MB macromolecular compound. The ConA-MB can effectively target periodontal pathogenic bacteria such as fusobacterium nucleatum and porphyromonas gingivalis, and the mechanism of the ConA-MB is that the ConA can specifically recognize lipopolysaccharide of an outer membrane of the bacteria and destroy bacterial cells, so that methylene blue enters the bodies of the bacteria, the targeted enrichment of the MB in the bacteria is improved, and the antibacterial effect is achieved. A photodynamic experiment proves that compared with pure MB, ConA-MB shows a higher combination speed and a stronger sterilization effect.

This invention provides a highly efficient method for purifying porcine epidemic diarrhea virus (PEDV), relating to the field of biology. The purification method includes the following steps: (1) adding concanavalin A and a precipitation promoter to the PEDV solution and mixing; (2) centrifuging and collecting the precipitate; (3) adding a dissociation solution to the precipitate and dissociating to obtain the purified virus solution. Using this method to purify PEDV, the impurity removal rate exceeds 90%, the recovery rate exceeds 90%, the viral load is large, and no high-value equipment is required.

To provide steamed and kneaded noodles that offer excellent workability during noodle making and are less prone to sticking together. [Solution] Steamed noodles containing 10% by mass or more of high-amylose wheat flour in the total mass of grain flours and starches in the raw material flour, wherein the high-amylose wheat flour is wheat flour in which the amylose content in the total starch, as analyzed by concanavalin A, is 40% by mass or more.

This invention discloses a nano-"sandwich" bacterial detection system with multiple SERS signal enhancement and its preparation method, belonging to the fields of functional material preparation and Raman detection. The nano-"sandwich" bacterial detection system includes highly adhesive labeled nanoparticles D-Au@Ag-C, whose structure consists of Au@Ag core-shell nanoparticles grown in situ on the surface of dendritic mesoporous silica DMSN, simultaneously modified with concanavalin A and a SERS tag; and a capture substrate Fe3O4@Au-Ab, whose structure consists of Fe3O4 nanoparticles with a gold coating on the surface, simultaneously modified with bacterial targeting antibodies. The nano-"sandwich" bacterial detection system of this invention can highly identify Staphylococcus aureus in aqueous solutions or blood, with extremely high detection accuracy and a very low detection limit, showing great promise for on-site detection of clinical bacterial infection-related diseases.

The application discloses a concanavalin A-like lectin / dextranase domain protein FvCLG and application thereof in improving production of fusarium venenatum mycelial protein, and belongs to the field of genetic engineering technology and food biotechnology. Fv CLG Compared with the wild type, the fusarium venenatum FG31 lacks a gene on the genome, does not produce ethanol on a fermenter for 72 h, has a significantly improved protein content (up to 61.34 %), and is 22.41 % higher than the wild type strain. Meanwhile, the sugar consumption of the FG31 strain during fermentation is significantly reduced, the glucose-to-biomass conversion rate and the glucose-to-protein conversion rate are respectively increased by 44.02 % and 76.41 %, the substrate utilization rate is significantly improved, the carbon source loss is reduced, and the production cost is reduced. The fusarium venenatum can be further used for gene engineering modification work, and a fusarium venenatum strain with high protein content is cultivated.

This invention relates to the fields of computational chemistry and enzyme engineering, specifically to a method for the targeted immobilization of nuclease P1 and a computationally-aided design-based method for the targeted affinity immobilization of nuclease P1. This invention, for the first time, combines computational and experimental methods, such as molecular dynamics simulations, to achieve precise affinity immobilization of nuclease P1 through targeted design. Based on the charge distribution and hydrophilic / hydrophobic regions of nuclease P1, combined with the spatial distribution of its key residues, dopamine and charged polymers are used as modifiers, co-deposited on the surface of a mesoporous resin support, exhibiting excellent adsorption capacity and immobilization effect. Furthermore, through dual optimization of glutaraldehyde crosslinking and concanavalin A modification, the binding efficiency and catalytic activity stability of nuclease P1 are significantly improved. This invention not only solves the problem of poor stability of nuclease P1 in the free state but also achieves efficient targeted affinity immobilization through novel molecular design and interface optimization techniques, providing a feasible technical path for industrial-scale applications.