The application discloses an air bacterial electrostatic collection and real-time
monitoring system and method integrated with a microfluidic portable sensor, and belongs to the technical field of
environmental monitoring, and comprises an
aerosol sampling module, a microfluidic
processing module, an electrochemical sensing module and a portable constant potential instrument module.
Bacteria aerosols in the
ambient air are charged by
corona discharge to realize efficient enrichment and collection; the enriched liquid sample is sent into the microfluidic
processing module and flows through a sensing
electrode modified with CuFeCoPBA@MoS2 nano
composite material, polydopamine and
concanavalin A. Target
bacteria are combined with the
concanavalin A on the surface of the
electrode in a broad-spectrum specific manner, the interface
electron transfer is hindered, the portable constant potential instrument collects the attenuation change of
differential pulse voltammetry electrochemical signals, and rapid quantitative detection of the concentration of air
bacteria is completed. The application integrates efficient air
aerosol collection and portable biosensing, and can realize one-step on-site capture and detection of air pathogens.