Non-enzymatic electrochemical bio-sensing method facilitating glucose detection

A technology for biosensing and glucose, applied in the field of bioelectrochemical sensing, can solve the problems of electrochemical electrical signal interference, complex preparation method and high analysis cost, and achieve the effects of low detection cost, overcoming complex preparation method and high sensitivity

Active Publication Date: 2017-12-01
HUBEI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a non-enzymatic electro-magnetic signal for the convenient detection of glucose in view of the problems that current blood glucose meters are usually interfered with by electrochemical signals from electroactive coexistent substances such as ascorbic acid, uric acid, dopamine, and dissolved oxygen in practical applications. Chemical biosensing method, this method is easy to operate, has the advantages of high sensitivity, wide linear range, and low cost, which overcomes the complex preparation method of traditional enzyme bioelectrochemical sensors, high analysis cost, and the solution is affected by the actual sample. Defects such as signal interference of active substances

Method used

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  • Non-enzymatic electrochemical bio-sensing method facilitating glucose detection
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  • Non-enzymatic electrochemical bio-sensing method facilitating glucose detection

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Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1 A non-enzymatic electrochemical biosensing method for conveniently detecting glucose, comprising the following steps:

[0020] (1) Preparation of electrochemical sensors

[0021] 1.8 μL of 1 mg / mL graphene oxide dispersion was added dropwise to the surface of the working electrode of the screen-printed carbon electrode, dried at room temperature and then transferred to Tris-HCl with a concentration of 50 mM pH=6.5, using the potentiostatic electrochemical reduction method, That is, the potential is -1.2V, the scanning rate is 100mV / s, and the time is 600s. The graphene oxide is converted into reduced graphene, washed with water and dried at room temperature to obtain the reduced graphene modified electrode; 2.0 μL of a 10 mM pH=7.4 Tris-HCl solution with a concentration of 3.0 μM phenyl-dextran was added dropwise to the surface of the alkene-modified electrode, washed at 37 °C for 2 h, washed and dried, and stored in a dry environment at 4 °C for later use....

Embodiment 2

[0024] Example 2 Detection of glucose content in standard solution

[0025] A mixed solution containing 20 μL of Au NP-Con A nanoprobe and 10 μL of glucose standard solution was added dropwise to the prepared sensor surface. After dynamic incubation at 37°C for 30min, 50mM pH=7.0 Tris-HCl / Tween-20 solution and Tris-HCl solution were used to wash and dry, and then 30μL of 0.1mol / L HCl solution was added dropwise, and pre-oxidized at 1.3V constant potential Immediately after 40s, the current response value was recorded by differential pulse voltammetry (DPV) in the range of 0.5-0V, so as to detect the content of glucose in the standard solution. The working curve of the obtained glucose standard solution is shown in Table 1 below.

[0026] Table 1 Working curve of glucose standard solution

[0027] Detector

Embodiment 3

[0028] Example 3 Detection of glucose content in human serum

[0029] Three clinical serum samples were taken, and five parallel tests were carried out for each sample. The processing method of the samples was the same as the above-mentioned standard solution. The concentration of glucose in the detected samples was shown in Table 2 below. The above-mentioned three clinical serum samples were analyzed by hospital commercialization. The measurement results of the instrument (Abbott C8000 automatic biochemical analyzer) are compared, and the results are shown in Table 2 below.

[0030] Table 2 Comparison of the measurement results of the present embodiment and the hospital commercialized analytical instrument

[0031] Sample serial number

[0032]As can be seen from Table 2 above, the relative standard deviation (RSD) of the test results of this embodiment is 4.9-6.7%, and the relative error is -3.9-6.4%, indicating that the analysis method of this embodiment has high ...

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Abstract

The invention discloses a non-enzymatic electrochemical bio-sensing method facilitating glucose detection. A silk-screen printing electrode is modified with graphene, phenyl-glucan is subjected to pai-pai stacking and assembled on the surface of the silk-screen printing electrode, a sensor is prepared, a prepared Con A (concanavalin A) functionalized Au NP (gold nanoparticle) probe is directly mixed with a glucose solution to be used for competitive recognition of the sensor surface to capture an Au NP marker, the Au NP on the electrode surface is detected and specifically recognized through electrochemical stripping analysis for signal transduction of the sensor, and thus, the content of glucan in the glucan solution is detected. The method is convenient to operate, has the advantages of high sensitivity, wide linear range, low cost and the like and overcomes the defects that traditional enzymatic bioelectrochemical sensor preparation methods are complex, the analysis cost is higher and solutions are interfered by signals of electroactive substances in actual samples and the like.

Description

technical field [0001] The invention relates to the technical field of bioelectrochemical sensing, in particular to a non-enzymatic electrochemical biosensing method for conveniently detecting glucose. Background technique [0002] Diabetes mellitus is a worldwide frequently-occurring disease that seriously endangers human health, and its main symptoms are metabolic disorders caused by hyperglycemia and insulin deficiency. Therefore, the development of glucose detection methods with excellent performance is of great significance for the clinical diagnosis and disease monitoring of diabetic patients. At present, the most widely used glucose biosensor is based on enzymatic reaction. The most basic principle is to use the immobilized glucose oxidase membrane as a biological recognition device to convert the detected amount of glucose into a usable output signal. Compared with traditional analytical methods such as photometry, chromatography and chemiluminescence, electrochemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 赖国松黎波
Owner HUBEI NORMAL UNIV
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