Application of calycosin-7-glucoside in preparation of immunity inhibitors and anti-inflammatory drugs

A technology of isoflavone and immunosuppressant, applied in the field of medicine, can solve the problems of unreported use and the like

Inactive Publication Date: 2014-10-22
PEKING UNIV
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Actascoside is an important compound in astragalus isoflavones, and its use in immunosuppression and anti-inflammation has not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of calycosin-7-glucoside in preparation of immunity inhibitors and anti-inflammatory drugs
  • Application of calycosin-7-glucoside in preparation of immunity inhibitors and anti-inflammatory drugs
  • Application of calycosin-7-glucoside in preparation of immunity inhibitors and anti-inflammatory drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Effect of Actascoisoflavone on Concanavalin A (ConA)-Induced Mouse T Cell Proliferation

[0011] 1 material

[0012] 1.1 Animals

[0013] BALB / c mice, male, weighing 18-20 g, were provided by the Experimental Animal Department of Peking University (permit number: SCXK11-00-0008). The animals were kept in a standard environment: temperature (23±1)°C, relative humidity (55±5)%, light and dark cycle 12h / 12h, and standard pellet feed.

[0014] 1.2 Reagents

[0015] Concanavalin A (Con A) and thiazolyl blue (MTT) were purchased from Sigma Company of the United States; fetal bovine serum (FBS) was purchased from GIBCO Company of the United States; red blood cell lysate, 1640 culture medium, and phosphate (PBS) buffer were purchased from From Beijing Maichen Biotechnology Company; DMSO (dimethyl sulfoxide, batch number 20101008) was purchased from Beijing Chemical Plant. Verbacosin samples were prepared in DMSO.

[0016] 1.3 Instruments

[0017] Bro-Rad iMark ...

Embodiment 2

[0028] Example 2: Effect of Actascoisoflavone on Macrophage Toxicity Caused by Lipopolysaccharide (LPS)

[0029] 1 material

[0030] 1.1 Reagents

[0031] Thiazolium blue (MTT), lipopolysaccharide (LPS, O11B4) were purchased from Sigma Company of the United States; fetal bovine serum (FBS) was purchased from GIBCO Company of the United States; DMEM culture medium and balanced salt solution (Hanks solution) were purchased from Beijing Maichen Biology Technology company; DMSO (dimethyl sulfoxide, batch number 20101008), purchased from Beijing Chemical Plant. Verbacosin samples were prepared in DMSO.

[0032] 1.2 Instruments

[0033] Bro-Rad iMark microplate reader (Bio-Rad, USA), Sigma3K-15 centrifuge (Sigma, Germany), CO 2 Incubator (Thermal Technology LLC of the United States), ultra-clean bench (Beijing Donglian Haer Instrument Manufacturing Co., Ltd.), etc.

[0034] 2 methods

[0035] Mouse macrophages (RAW264.7) were cultured with DMEM (containing 10% FBS). After the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Through the experiments and researches on calycosin-7-glucoside, it has been found for the first time that calycosin-7-glucoside can prominently inhibit the T lymphocyte proliferation induced by concanavalin A (Con A) and reduce the macrophage toxicity induced by lipopolysaccharide (LPS), has immunity inhibiting and anti-inflammatory effects, and is advantageously used to prepare immunity inhibitors and anti-inflammatory drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of verbascoside in the preparation of immunosuppressants and anti-inflammatory drugs. Background technique [0002] Astragalus membranaceus is a traditional Chinese medicine that strengthens the spleen and replenishes qi. It has the functions of invigorating qi and solidifying the surface, diuretic and detoxification, draining pus, astringing sores and promoting granulation. Hematochezia and metrorrhagia, surface deficiency and spontaneous sweating, Qi deficiency and edema, carbuncle and gangrene, persistent ulceration, blood deficiency and yellowish yellow, internal heat and thirst, chronic nephritis, proteinuria and diabetes (see: National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China ( Part One) [z]. Beijing: Chemical Industry Press, 2005: 212-213.). Modern pharmacological studies believe that Astragalus can regulate the immune function of the body a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P37/06A61P29/00
Inventor 徐风叶加张微微蔡少青
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap