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Bioactive polypeptide SLPQ and its preparation method and use

A technology of bioactive polypeptides and polypeptides, applied in the field of proteins, can solve the problems of loss of physiological activity and inability to achieve, and achieve the effects of improving the ability of infection, promoting the amount of induction, and enhancing the body's immunity

Active Publication Date: 2015-06-10
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the complex environment of the human gastrointestinal tract, peptides are easily degraded by a series of enzymes such as pepsin and trypsin, thus losing their physiological activity in the body and failing to achieve the intended purpose

Method used

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  • Bioactive polypeptide SLPQ and its preparation method and use
  • Bioactive polypeptide SLPQ and its preparation method and use
  • Bioactive polypeptide SLPQ and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 active peptide

[0047] 1. Preparation of fermented milk

[0048] 1) Lactobacillus helveticus fermented milk

[0049] Use skimmed milk powder (New Zealand NZMP brand skimmed milk powder) and water to prepare 12wt% skimmed milk (12g skimmed milk powder is added to 88g water, the same below). Under aseptic conditions, the tricyclic colony of Lactobacillus helveticus (CICC6024) was picked, added to sterilized 12wt% skim milk, and stirred evenly. After inoculation, seal with aluminum foil to prevent contamination. Place in an incubator at 37°C for 19 hours. After the cultivation, the curd is evenly stirred to complete the activation of Lactobacillus helveticus, and a starter for preparing Lactobacillus helveticus fermented milk is obtained.

[0050] Inoculate 10mL of the prepared Lactobacillus helveticus starter into 500mL of sterilized 12% (v / v) skim milk (inoculation rate 2v / v%), ferment at 37°C for 19 hours, stir the curd, Store at 4°...

Embodiment 2

[0123] Example 2 Bioactive Peptide Promoting Body Immunity Activity Experiment

[0124] 1. In vitro lymphocyte proliferation ability test of biologically active polypeptide SLPQ by MTT method

[0125] 1) Experimental materials and instruments:

[0126] Reagents and materials: experimental animals balb / c mice (male 6-8 weeks old, Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University); milk-derived bioactive polypeptide SLPQ separated by mass spectrometry after fermentation of Lactobacillus helveticus; Rat lymphocyte extract (purchased from Suolaibao); RPMI1640 medium (purchased from GIBCO); 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide Salt (MTT, purchased from Amresco); concanavalin (ConA, purchased from Sigma); bovine serum albumin (BSA, purchased from Genebase); pepsin (purchased from Sigma); purchased from AB Company).

[0127] Instruments: LRH-250F biochemical incubator, Shanghai Heng Technology Co., Ltd.; GL-22M high-spe...

Embodiment 3

[0167] Embodiment 3 Biologically active polypeptide SLPQ gastrointestinal digestion stability

[0168] (1) In vitro simulation of gastrointestinal digestion

[0169] The simulated gastrointestinal digestion experiment is mainly divided into two steps. First, prepare a bioactive polypeptide SLPQ solution with a concentration of 500 μg / mL, add pepsin to the SLPQ solution with a concentration of 500 μg / mL, and add 20 mg of pepsin per gram of SLPQ, adjust the pH value of the reaction solution to 2.0, and keep the temperature at 37°C Incubate in a water bath for 90 minutes; then adjust the pH value of the reaction solution to 7.5, add trypsin at a rate of 40 mg per gram of SLPQ, and incubate in a constant temperature water bath at 37°C for 150 minutes; finally heat in a water bath at 95°C for 5 minutes to digest The enzyme was inactivated, and the reaction solution was freeze-concentrated and dried to make a dry powder, which was stored at -20°C for future use.

[0170] (2) Deter...

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Abstract

Provided is a polypeptide, the amino acid sequence of which is SLPQ. The polypeptide is prepared by fermented skimmed milk by Lactobacillus helveticus. The polypeptide can enhance immunity of a body, so also provided are food, health products and medicines comprising the polypeptide.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a milk-derived biologically active polypeptide SLPQ and its preparation and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] Immunoactive peptides are a class of biologically active peptides obtained from milk for the first time after the discovery of opioid peptides and their physiological activity has been proved. In 1981, Jolles et al. first discovered th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/103C07K1/20C12N15/11C12P21/02A23L1/29A61K38/07A61P37/04A23L33/00
CPCA61K38/00A61P37/04C07K5/1013C12P21/02C12P21/06
Inventor 张少辉卢姗姗孙冠华马鎏镠周婕慧郭海滨胡迪
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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