A kind of bioactive peptide iahpklgkrir and its preparation method and application

A technology of biologically active peptides and sequences, applied in the protein field, to achieve the effects of promoting in vitro proliferation, improving quality, and reducing morbidity

Active Publication Date: 2022-05-27
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of 60S ribosomal protein L29 protein polypeptide fragments in the prior art

Method used

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  • A kind of bioactive peptide iahpklgkrir and its preparation method and application
  • A kind of bioactive peptide iahpklgkrir and its preparation method and application
  • A kind of bioactive peptide iahpklgkrir and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Artificial synthesis of bioactive peptide IAHPKLGKRIR

[0033] 1. Synthesis of Bioactive Peptides

[0034] Synthetic bioactive peptide IAHPKLGKRIR.

[0035] 2. Confirmation of Bioactive Peptides

[0036] 1) UPLC analysis

[0037] The UPLC conditions are as follows:

[0038] Instrument: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer

[0039] Column Specifications: BEH C18 Column

[0040] Flow rate: 0.4mL / min

[0041] Temperature: 50℃

[0042] UV detection wavelength: 210nm

[0043] Injection volume: 2μL

[0044] Gradient conditions: solution A: water containing 0.1% formic acid (v / v), solution B: acetonitrile containing 0.1% formic acid (v / v)

[0045] Time(min) %A %B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0046] 2) Mass spectrometry

[0047] The mass spectrometry conditio...

Embodiment 2

[0061] Example 2 Immunoactivity test of biologically active peptides

[0062] 1. The ability of bioactive peptide IAHPKLGKRIR to stimulate macrophage phagocytosis of neutral red

[0063] 1. Experimental reagents and instruments:

[0064] Reagents: experimental animal balb / c mice (male, 6-8 weeks old), Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University; the biologically active peptide IAHPKLGKRIR derived from mouse spleen lymphocytes obtained in Example 1; LPS, purchased from Sigma Company ; Neutral red staining solution, produced by Biyuntian Institute of Biotechnology.

[0065] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO2 incubator Heraeus Company; DragonWellscan MK3 Microplate Reader Labsystems Company.

[0066] 2. Experimental method:

[0067] The number of cells added is 2 × 10 6 100μl / we...

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Abstract

The present invention relates to the field of proteins, in particular to a bioactive peptide IAHPKLGKRIR and its preparation method and application. The amino acid sequence of the bioactive peptide IAHPKLGKRIR is Ile‑Ala‑His‑Pro‑Lys‑Leu‑Gly‑Lys‑Arg‑Ile‑Arg . In vitro immune function regulation experiments proved that the biologically active peptide IAHPKLGKRIR has a good immune regulation function. The biologically active peptide IAHPKLGKRIR of the present invention can promote the proliferation ability of lymphocytes in vitro, improve the immunity of the human body, and at the same time have a significant promotion effect on the ability of macrophages to phagocytize neutral red in vitro, improve the body's ability to resist external pathogen infection, reduce the body's It is of great significance to develop food, health care products and medicines with immune regulation function and improve the quality of life.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide IAHPKLGKRIR and a preparation method and application thereof. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anticancer, blood pressure lowering, antibacterial, cholesterol lowering, antidiabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] At present, the research on bioactive peptides mostly focuses on food-derived peptides, and there are few studies and reports on non-food-derived peptides. And research results have confirmed that, compared with f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉王娟栾媛媛占文静张伯宇郭婷婷谢雨佳李政
Owner SHANGHAI JIAO TONG UNIV
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