A kind of bioactive peptide iahpklgkrir and its preparation method and application
A technology of biologically active peptides and sequences, applied in the protein field, to achieve the effects of promoting in vitro proliferation, improving quality, and reducing morbidity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Example 1 Artificial synthesis of bioactive peptide IAHPKLGKRIR
[0033] 1. Synthesis of Bioactive Peptides
[0034] Synthetic bioactive peptide IAHPKLGKRIR.
[0035] 2. Confirmation of Bioactive Peptides
[0036] 1) UPLC analysis
[0037] The UPLC conditions are as follows:
[0038] Instrument: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer
[0039] Column Specifications: BEH C18 Column
[0040] Flow rate: 0.4mL / min
[0041] Temperature: 50℃
[0042] UV detection wavelength: 210nm
[0043] Injection volume: 2μL
[0044] Gradient conditions: solution A: water containing 0.1% formic acid (v / v), solution B: acetonitrile containing 0.1% formic acid (v / v)
[0045] Time(min) %A %B
0 95.0 5.0
1.50 80.0 20.0
3.50 60.0 40.0
5.00 40.0 60.0
7.00 15.0 85.0
8.00 0.0 100.0
11.00 0.0 100.0
11.50 95.0 5.0
13.00 95.0 5.0
[0046] 2) Mass spectrometry
[0047] The mass spectrometry conditio...
Embodiment 2
[0061] Example 2 Immunoactivity test of biologically active peptides
[0062] 1. The ability of bioactive peptide IAHPKLGKRIR to stimulate macrophage phagocytosis of neutral red
[0063] 1. Experimental reagents and instruments:
[0064] Reagents: experimental animal balb / c mice (male, 6-8 weeks old), Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University; the biologically active peptide IAHPKLGKRIR derived from mouse spleen lymphocytes obtained in Example 1; LPS, purchased from Sigma Company ; Neutral red staining solution, produced by Biyuntian Institute of Biotechnology.
[0065] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO2 incubator Heraeus Company; DragonWellscan MK3 Microplate Reader Labsystems Company.
[0066] 2. Experimental method:
[0067] The number of cells added is 2 × 10 6 100μl / we...
PUM
Property | Measurement | Unit |
---|---|---|
ion source temperature | aaaaa | aaaaa |
collision energy | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com