A kind of bioactive peptide saprhgslgflprk and its preparation method and application

A technology of biologically active peptides and drugs, applied in the protein field, to achieve the effects of promoting in vitro proliferation, good anti-inflammatory activity, and improving the ability of

Active Publication Date: 2022-05-31
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of 60S ribosomal protein L3 protein polypeptide fragments in the prior art

Method used

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  • A kind of bioactive peptide saprhgslgflprk and its preparation method and application
  • A kind of bioactive peptide saprhgslgflprk and its preparation method and application
  • A kind of bioactive peptide saprhgslgflprk and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Example 1 Artificial synthesis of active peptide SAPRHGSLGFLPRK

[0034] 1. Synthesis of Bioactive Peptides

[0035] 1. Weigh 3 g of RINK resin (degree of substitution: 0.3 mmol / g) into a 150 ml reactor, and soak it with 50 ml of dichloromethane (DCM).

[0036] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the resin volume, and then drained. This was repeated four times, and the resin was drained for use.

[0037] 3. A certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) was added to the reactor, and it was shaken on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, it was washed four times with 3 times the resin volume of DMF, and then drained.

[0038] 4. Take a small amount of resin and test it by ninhydrin (Ninhydrin hydrate) method (two drops each of test A and test B, react at 100°C for 1 min), the resin is colored, indicating that the deprotection is successful. ...

Embodiment 2

[0075] Example 2 Immunological Activity Experiment of Bioactive Peptides

[0076] 1. MTT assay to determine the in vitro macrophage proliferation ability of the bioactive peptide SAPRHGSLGFLPRK

[0077] 1. Experimental reagents and instruments

[0078] Reagents: experimental animals balb / c mice (male 6-8 weeks old) Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University; the mouse spleen lymphocyte-derived bioactive peptide SAPRHGSLGFLPRK obtained in Example 1; 3-(4,5 -Dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) Amresco; LPS (Lipopolysaccharide) Sigma; Bovine Serum Albumin (BSA) Genebase; Triplex Dissolving solution, an aqueous solution containing 10% SDS, 5% isobutanol and 0.012 mol / L HCl.

[0079] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus Company; Dragon Wellsc...

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Abstract

The present invention relates to the field of proteins, in particular to a bioactive peptide SAPRHGSLGFLPRK and its preparation method and application. The amino acid sequence of the bioactive peptide SAPRHGSLGFLPRK is Ser‑Ala‑Pro‑Arg‑His‑Gly‑Ser‑Leu‑Gly‑Phe‑Leu ‑Pro‑Arg‑Lys. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide SAPRHGSLGFLPRK has a good immune regulation function. The biologically active peptide SAPRHGSLGFLPRK of the present invention can significantly promote the in vitro proliferation ability of macrophages, and at the same time increase the ability to induce nitric oxide, thereby improving the body's ability to resist external pathogen infection, reducing the body's morbidity rate, and improving life expectancy. Quality is of great significance to the development of food, health care products and medicines with immune regulation function.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide SAPRHGSLGFLPRK and a preparation method and application thereof. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have been well proven, such as anticancer, blood pressure lowering, antibacterial, cholesterol lowering, antidiabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] Immunomodulatory peptides generally refer to small peptides with relatively small molecular weights that have immunomodulatory activity. The immunomodulatory peptides disclosed now are generally small peptides with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55Y02A50/30
Inventor 张少辉占文静张伯宇
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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