Cell culture medium, cell culture kit and cell culture method
A cell culture and culture medium technology, applied in the field of cell culture medium, can solve the problems of fast aging and death, death and other problems
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Embodiment 1
[0096] This example provides a sebocyte culture medium, which contains DMEM / Ham'F-12 (1 / 1), 0.2 mg / mL casein, 1.2 μg / mL transferrin, 12 μg / mL mL of bovine insulin, 1 μg / mL of linoleic acid, 400nmol / L of testosterone, 0.12mmol / L of vitamin A, 0.04mmol / L of L-ascorbic acid, 0.13mmol / L of polylysine, 0.4nmol / L of putrescine and 25 μmol / L of 2-mercaptoethanol.
[0097] The preparation method of the sebocyte culture medium of the present embodiment is as follows:
[0098] In a sterile clean bench, add 12 μg / mL bovine insulin, 0.2 mg / mL casein, 1.2 μg / mL trans Ferritin, 1μg / mL linoleic acid, 400nmol / L testosterone, 0.12mmol / L vitamin A, 0.04mmol / L L-ascorbic acid, 0.13mmol / L polylysine, 0.4nmol / L putrid Amine and 25 μmol / L 2-mercaptoethanol, mixed by pipetting, and sterilized by positive pressure filtration with a microporous filter membrane with a pore size of 0.22 μm.
Embodiment 2
[0100] This example provides a sebocyte culture medium, which contains DMEM / Ham'F-12 (1 / 1), 5 mg / mL casein, 5 μg / mL transferrin, 30 μg / mL Bovine insulin, 5μg / mL linoleic acid, 800nmol / L testosterone, 0.28mmol / L vitamin A, 0.28mmol / L L-ascorbic acid, 0.26mmol / L polylysine, 2.5nmol / L putrid amine and 120 μmol / L 2-mercaptoethanol.
[0101] The preparation method of the sebocyte culture medium of the present embodiment is as follows:
[0102] In a sterile clean bench, add 30 μg / mL bovine insulin, 5 mg / mL casein, and 5 μg / mL transferrin to the culture medium with a volume ratio of DMEM:Ham'F-12 of 1:1 , 5μg / mL of linoleic acid, 800nmol / L of testosterone, 0.28mmol / L of vitamin A, 0.28mmol / L of L-ascorbic acid, 0.26mmol / L of polylysine, 2.5nmol / L of putrescine and 120 μmol / L 2-mercaptoethanol, pipette and mix well, and use a microporous filter membrane with a pore size of 0.22 μm to filter and sterilize under positive pressure.
Embodiment 3
[0104] This example provides a sebocyte culture medium, which contains DMEM / Ham'F-12 (1 / 1), 0.5 mg / mL casein, 1.5 μg / mL transferrin, 15 μg / mL mL of bovine insulin, 5μg / mL of linoleic acid, 400nmol / L of testosterone, 0.15mmol / L of vitamin A, 0.05mmol / L of L-ascorbic acid, 0.26mmol / L of polylysine, 1nmol / L of Putrescine and 25 μmol / L 2-mercaptoethanol.
[0105] The preparation method of the sebocyte culture medium of the present embodiment is as follows:
[0106] In a sterile clean bench, add 15 μg / mL bovine insulin, 0.5 mg / mL casein, 1.5 μg / mL trans Ferritin, 5 μg / mL linoleic acid, 400 nmol / L testosterone, 0.15 mmol / L vitamin A, 0.05 mmol / L L-ascorbic acid, 0.26 mmol / L polylysine, 1 nmol / L putrescine and 25 μmol / L 2-mercaptoethanol, mixed by pipetting, and sterilized by positive pressure filtration with a microporous filter membrane with a pore size of 0.22 μm.
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