Culture medium containing human umbilical cord mesenchymal stem cell exudates and preparation method and applications thereof

A technology of stem cells and culture medium, applied in the field of biomedicine, can solve the problems of unclear mechanism and many disputes, and achieve the effect of inhibiting the apoptosis of damaged liver cells, the method is simple, and the regeneration of liver cells is stimulated.

Inactive Publication Date: 2012-09-19
斯坦姆生物科技江苏有限公司
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Problems solved by technology

[0003] MSCs have made gratifying progress in the treatment of acute myocardial infarction, stroke, acute kidney injury, diabetes and fractures in clinical and subclinical trials, but the specific mechanism is still not very clear
MSCs can be induced to differentiate into tissue cells of the three germ layers of bone, cartilage, skeletal muscle, cardiac muscle, vascular endothelium, glial cells, and insulin-secreting cells in vitro. Therefore, MSCs transplanted into the body differentiate into functional cells to replace damaged cells. It is considered to be the main mechanism of transplantation therapy, but with the in-depth study of the characteristics of MSCs, there are many controversies about the mechanism

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  • Culture medium containing human umbilical cord mesenchymal stem cell exudates and preparation method and applications thereof
  • Culture medium containing human umbilical cord mesenchymal stem cell exudates and preparation method and applications thereof
  • Culture medium containing human umbilical cord mesenchymal stem cell exudates and preparation method and applications thereof

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Embodiment Construction

[0022] The present invention will be described in further detail below in conjunction with specific examples, and the following examples are only descriptive, not limiting, and cannot limit the protection scope of the present invention with this.

[0023] The percentages involved in this embodiment are all percentages by weight if not specifically limited. The cell culture medium used in the present invention is common knowledge in the field and has no particularity.

[0024] A preparation method of a culture medium containing human umbilical cord mesenchymal stem cell secretion, the steps are as follows:

[0025] (1) Collect the umbilical cord cells and carry out primary culture. Adherent cells can be observed within 24 hours. After growing to 10 days, the cells can be seen to cover the bottom of the bottle. Subculture is carried out once a week, and the 5th-9th generation cells are taken. The expression of CD29, CD49, CD90, CD105, and HLA-1 was detected by flow cytometry, a...

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Abstract

The invention relates to a culture medium containing human umbilical cord mesenchymal stem cell exudates and a preparation method and applications thereof. An umbilical cord mesenchymal stem cell finite cell line is established to identify biological characteristics of the umbilical cord mesenchymal stem cell, and the preparation method for the human umbilical cord mesenchymal stem cell exudates is established to verify that the human umbilical cord mesenchymal stem cell (HUCMSC) exudates can promote cell regeneration, improve cell function and suppress cell apoptosis in vitro. A detection of enzyme-linked immunosorbent assay (ELISA) shows that human umbilical cord mesenchymal stem cell exudates contain abundant cell factors which can promote cell proliferation and suppress cell apoptosis, such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF).

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a culture medium containing secretion fluid of human umbilical cord mesenchymal stem cells and its preparation method and application. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm with self-replication, multi-lineage differentiation and immune regulation capabilities, and have good application prospects in the fields of tissue engineering, genetic engineering and stem cell transplantation. At present, MSCs have been isolated from tissues such as bone marrow, fat, embryonic liver, synovium, amniotic fluid, placenta, umbilical cord blood and umbilical cord. Among them, umbilical cord is medical waste with rich sources, no adverse effects on donors, and no ethical issues. Human umbilical cord MSCs are rich in content, relatively primitive, and have strong differentiation ability. They can be isolated and cultured in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 朱争艳任峰
Owner 斯坦姆生物科技江苏有限公司
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