A kind of bioactive peptide ggygsgggsggygsrrf and its preparation method and application

A bioactive peptide and drug technology, applied in the protein field to enhance in vitro proliferation ability, inhibit inflammation, and improve the quality of life

Active Publication Date: 2022-05-31
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of polypeptide fragments of Heterogeneous nuclear ribonucleoprotein A3 protein in the prior art

Method used

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  • A kind of bioactive peptide ggygsgggsggygsrrf and its preparation method and application
  • A kind of bioactive peptide ggygsgggsggygsrrf and its preparation method and application
  • A kind of bioactive peptide ggygsgggsggygsrrf and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Artificial synthesis of active peptide GGYGSGGGSGGYGSRRF

[0033] 1. Synthesis of Bioactive Peptides

[0034] 1. Weigh 3 g of RINK resin (degree of substitution: 0.3 mmol / g) into a 150 ml reactor, and soak it with 50 ml of dichloromethane (DCM).

[0035] 2. After 2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) of 3 times the resin volume, and then drained. This was repeated four times, and the resin was drained for use.

[0036] 3. A certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) was added to the reactor, and it was shaken on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, it was washed four times with 3 times the resin volume of DMF, and then drained.

[0037] 4. Take a small amount of resin and test it by ninhydrin (Ninhydrin hydrate) method (two drops each of test A and test B, react at 100°C for 1 min), the resin is colored, indicating that the deprotection is succ...

Embodiment 2

[0075] Example 2 Immunological Activity Experiment of Bioactive Peptides

[0076] 1. MTT assay to determine the in vitro macrophage proliferation ability of the bioactive peptide GGYGSGGGSGGYGSRRF

[0077] 1. Experimental reagents and instruments:

[0078] Reagents: experimental animals balb / c mice (male 6-8 weeks old) Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University; the mouse spleen lymphocyte-derived bioactive peptide GGYGSGGGSGGYGSRRF obtained in Example 1; 3-(4,5 -Dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) Amresco; LPS (Lipopolysaccharide) Sigma; Bovine Serum Albumin (BSA) Genebase; Triplex Dissolving solution, an aqueous solution containing 10% SDS, 5% isobutanol and 0.012 mol / L HCl.

[0079] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus Company; Dragon ...

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Abstract

The present invention relates to the field of proteins, in particular to a bioactive peptide GGYGSGGGSGGYGSRRF and its preparation method and application. The amino acid sequence of the bioactive peptide GGYGSGGGSGGYGSRRF is Gly-Gly-Tyr-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly ‑Tyr‑Gly‑Ser‑Arg‑Arg‑Phe. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide GGYGSGGGSGGYGSRRF has a good immune regulation function. The biologically active peptide GGYGSGGGSGGYGSRRF of the present invention can significantly enhance the in vitro proliferation ability of macrophages, can effectively inhibit inflammation caused by oxidation, has a good immune regulation effect, can improve the body's ability to resist external pathogen infection, and reduce the body's morbidity , improving the quality of life is of great significance to the development of food, health products and medicines with immune regulation function.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide GGYGSGGGSGGYGSRRF and a preparation method and application thereof. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anticancer, blood pressure lowering, antibacterial, cholesterol lowering, antidiabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] At present, the research on bioactive peptides mostly focuses on food-derived peptides, and there are few studies and reports on non-food-derived peptides. And research results have confirmed that, compared ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A23L33/18
CPCY02A50/30
Inventor 张少辉张伯宇占文静
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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