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Bioactive peptide SEPKPIFF as well as preparation method and application thereof

A biologically active peptide and active technology, applied in the protein field, can solve the problems related to the functional research of ProteinSON protein polypeptide fragments, achieve good anti-inflammatory activity, enhance in vitro proliferation ability, and inhibit inflammation

Active Publication Date: 2021-04-20
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Protein SON protein fragments in the prior art

Method used

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  • Bioactive peptide SEPKPIFF as well as preparation method and application thereof
  • Bioactive peptide SEPKPIFF as well as preparation method and application thereof
  • Bioactive peptide SEPKPIFF as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The artificial synthesis of embodiment 1 active peptide SEPKPIFF

[0032] 1. Synthesis of Bioactive Peptides

[0033] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0034] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0035] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0036] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0037]5. We...

Embodiment 2

[0073] The immune activity experiment of embodiment 2 bioactive peptides

[0074] 1. The effect of bioactive peptide SEPKPIFF on immune cytokines in serum

[0075] 1. Experimental reagents and instruments:

[0076] Reagents: experimental animal ICR mice (male 5 weeks old), Shanghai Experimental Animal Center; D-gal, Sinopharm Chemical Reagent Co., Ltd.; paraformaldehyde, Sinopharm Chemical Reagent Co., Ltd.; sodium chloride, Sinopharm Chemical Reagent Co., Ltd. Co., Ltd.; mouse spleen lymphocyte-derived bioactive peptide SEPKPIFF obtained in Example 1; BCA protein kit, Nanjing Kaiji Biotechnology Co., Ltd.; ELISA cytokine rapid kit (TNF-α and IL-6), Dr. Wuhan Germany Bioengineering Co., Ltd.

[0077] Instruments and equipment: CM-230 Mole ultra-clean water, Shanghai Moller Scientific Instrument Co., Ltd.; MilliporeMILLEX GP0.22μm filter membrane, Millipore Corporation; GL-22M high-speed refrigerated centrifuge, Shanghai Lu Xiangyi Centrifuge Instrument Co., Ltd.

[0078] 2....

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Abstract

The invention relates to the field of protein, in particular to a bioactive peptide SEPKPIFF as well as a preparation method and application thereof, and the amino acid sequence of the bioactive peptide SEPKPIFF is Ser-Glu-Pro-Lys-Pro-Ile-Phe. In-vitro immunoregulation function verification experiments prove that the bioactive peptide SEPKPIFF has a very good immunoregulation function. The bioactive peptide SEPKPIFF can enhance the in-vitro proliferation capacity of lymphocytes, can effectively inhibit inflammation caused by oxidation of a body, improves the capacity of the body for resisting external pathogen infection, reduces the morbidity of the body, is greatly helpful to treatment of chronic diseases caused by inflammation, and has very important significance in developing food, health care products and medicines with an immunoregulation function.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a bioactive peptide SEPKPIFF and its preparation method and application. Background technique [0002] Immunomodulatory peptides generally refer to small peptides with relatively small molecular weights that have immunomodulatory activity. Immunomodulatory peptides are a class of bioactive peptides obtained from milk for the first time after the discovery of opioid peptides and their physiological activity has been proved. In 1981, Jolles et al. first discovered that a hexapeptide with the amino acid sequence Val-Glu-Pro-Ile-Pro-Tyr could be obtained by hydrolyzing human milk protein with trypsin. In vitro experiments proved that this peptide can enhance mouse peritoneal macrophages Phagocytosis of sheep erythrocytes. Migliore-Samour et al found that the hexapeptide Thr-Thr-Met-Pro-Leu-Trp from casein can stimulate the phagocytosis of sheep red blood cells to mouse peritoneal macrophage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P29/00A61P37/02A61K8/64A61Q19/00A23L33/18
CPCY02P20/55
Inventor 张少辉占文静张伯宇
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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