New application of vitamin D

A vitamin and skin cell technology, applied in the field of biomedicine, can solve the problems of high mortality rate, low early screening and diagnosis rate, and high degree of malignancy of ovarian cancer

Pending Publication Date: 2015-11-25
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ovarian cancer is a gynecological cancer with a very high mortality rate, which is mainly related to three characteristics: first, ovarian cancer lacks effective screening and diagnostic measures in the early stage; second, ovarian cancer is highly malignant and prone to metastasis in the late stage; third, In advanced ovarian cancer, cancer cells are prone to drug resistance to chemotherapy drugs such as carboplatin and paclitaxel
Ovarian cancer is very prone to distant metastasis, the early screening and diagnosis rate is low, and it is easy to develop resistance to radiotherapy and chemotherapy in the late stage, and the mortality rate is extremely high. Therefore, the discovery of preventive treatment drugs for ovarian cancer is imminent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Active vitamin D inhibits the in vitro proliferation ability of ovarian surface epithelial cells

[0062] 1.1 The effect of active vitamin D on the colony formation ability of MOSEC plates

[0063] Clonogenic assays or colony formation assays are used to determine the ability of individual cells to grow into colonies, reflecting cell population dependence and proliferation. To detect 1,25(OH) 2 D. 3 Whether it affects the ability of cell plate colony formation during the malignant transformation of MOSEC, the present invention takes cells from the control group and the active vitamin D treatment group every 10 generations, and compares the difference in the colony formation ability of the two groups of cells. In the early stage of MOSEC culture in vitro, the cell growth is extremely slow, and half the volume of medium needs to be changed. This experiment starts from the 20th generation (use P20, the following analogy), using 1nM 1,25(OH) 2 D. 3 Treat MOSE...

Embodiment 2

[0093] Example 2 Active vitamin D weakens the in vivo tumorigenic ability of ovarian surface epithelial cells

[0094] 2.1 Experimental method

[0095] experimental animals

[0096] SPF-grade immunodeficient BALB / C mice, aged 4-6 weeks, were raised in a 12-hour light / 12-hour dark animal room with sufficient water and feed. SPF grade BALB / C nude mice were obtained from the Experimental Animal Center of Soochow University. They were raised in the SPF Experimental Animal Center of Soochow University, and adaptively fed for 2 weeks, then ears were cut, marked, and grouped. After intraperitoneal injection of cells, the body weight and body temperature were measured once a week, and the general signs, activities, and tumor development of the mice were observed, and records were made.

[0097] In vivo tumor formation experiment

[0098] Tumor formation experiments in nude mice were performed in accordance with the protocol approved by IACUC (Institutional Animal Care and Use Comm...

Embodiment 3

[0119] Example 3 Active vitamin D inhibits the in vitro migration ability of ovarian surface epithelial cells

[0120] In order to observe 1,25(OH) 2 D. 3 Whether to inhibit the migration ability of MOSEC during long-term treatment, the present invention uses Transwell and scratch experiments to detect the migration ability of MOSEC. The experimental method is as follows:

[0121] Transwell Migration Experiment

[0122]Microfiltration membrane culture chambers were used to examine the migration ability of MOSEC and the effect of long-term treatment of active vitamin D on migration ability. There are 8 μm micropores on the filter membrane to allow cells to pass through to reach the bottom of the membrane. These passing cells can adhere to the bottom of the membrane, and the migration situation can be reflected by the number of adhered cells. Specific experimental steps: starve the two groups of MOSECs one day before the experiment, digest the starved cells the next day, cou...

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Abstract

The invention relates to a method for slowing down ovarian surface epithelial cell vicious transformation. During ovarian surface epithelial cell vicious transformation, active vitamin D (1,25(OH)2D3) is added through each passage and after each culture medium replacement so as to slow down the ovarian surface epithelial cell vicious transformation. The invention further provides application of the vitamin D in the preparation of medicine for treating ovarian cancer. By the long-term use of low dosage of vitamin D, 25-hydroxyvitamin D or active vitamin D, the tumorigenic ability of ovarian surface epithelial cells in the body of a nude mouse can be weakened effectively.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the new application of vitamin D. Background technique [0002] According to the new data released by the Chinese Cancer Society on February 15, 2015, in 2011 alone, the incidence and death toll of ovarian cancer in China were 45,223 and 18,430, respectively. Ovarian Surface Epithelial Cell (OSEC) is a layer of cubic-rectangular cells coated on the surface of the ovary, located between the ovarian ligament and peritoneal mesothelium, and originated from the Müllerian system. After the ovary ovulates, it plays a role in repairing the surface ulceration of the ovary. [0003] According to the origin, ovarian cancer is mainly divided into three types: 1. epithelial-mesenchymal; 2. sex cord-mesenchymal; 3. germ cell tumors. According to current research, epithelial tumors account for about 60% of all ovarian tumors, and about 90% of malignant ovarian tumors are considered to originate fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071A61K31/59A61K31/592A61K31/593A61P35/00
Inventor 李冰燕王萍张鹤美张增利刘利芝侯永凤胡志勇
Owner SUZHOU UNIV
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