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A kind of bioactive peptide kqslppglavkdlk and its preparation method and application

A technology of biologically active peptides and drugs, applied in the field of protein, to achieve good anti-inflammatory activity, improve the ability of the body, and enhance the effect of in vitro proliferation

Active Publication Date: 2022-05-24
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of 60S ribosomal protein L38 protein polypeptide fragments in the prior art

Method used

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  • A kind of bioactive peptide kqslppglavkdlk and its preparation method and application
  • A kind of bioactive peptide kqslppglavkdlk and its preparation method and application
  • A kind of bioactive peptide kqslppglavkdlk and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Synthesis of biologically active peptide KQSLPGLAVKDLK

[0030] 1. Synthesis of Bioactive Peptides

[0031] The bioactive peptide KQSLPGLAVKDLK was synthesized artificially.

[0032] 2. Confirmation of Bioactive Peptides

[0033] 1) UPLC analysis

[0034] The UPLC conditions are as follows:

[0035] Instrument: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer

[0036] Column Specifications: BEH C18 Column

[0037] Flow rate: 0.4mL / min

[0038] Temperature: 50℃

[0039] UV detection wavelength: 210nm

[0040] Injection volume: 2μL

[0041] Gradient conditions: solution A: water containing 0.1% formic acid (v / v), solution B: acetonitrile containing 0.1% formic acid (v / v)

[0042] Time(min) %A %B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0043] 2) Mass spectrometry

[0044] The ...

Embodiment 2

[0058] Example 2 Immunoactivity test of biologically active peptides

[0059] 1. MTT assay to determine the in vitro macrophage proliferation ability of the biologically active peptide KQSLPGLAVKDLK

[0060] 1. Experimental reagents and instruments

[0061] Reagents: experimental animals balb / c mice (male 6-8 weeks old) Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University; the biologically active peptide KQSLPGLAVKDLK derived from mouse spleen lymphocytes obtained in Example 1; 3-(4,5 -Dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) Amresco; LPS (Lipopolysaccharide) Sigma; Bovine Serum Albumin (BSA) Genebase; Triplex Dissolving solution, an aqueous solution containing 10% SDS, 5% isobutanol and 0.012 mol / L HCl.

[0062] Equipment: LRH-250F biochemical incubator, Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus...

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Abstract

The present invention relates to the field of proteins, in particular to a bioactive peptide KQSLPPGLAVKDLK and its preparation method and application. The amino acid sequence of the bioactive peptide KQSLPPGLAVKDLK is Lys‑Gln‑Ser‑Leu‑Pro‑Pro‑Pro‑Gly‑Leu‑Ala‑Val‑Lys ‑Asp‑Leu‑Lys. The in vitro immune function regulation experiment proved that the biologically active peptide KQSLPPGLAVKDLK has a good immune regulation function. The biologically active peptide KQSLPPGLAVKDLK of the present invention has the ability to enhance the in vitro proliferation of macrophages and lymphocytes, improve the ability of the body to resist external pathogen infection, reduce the incidence of the body, improve the quality of life, and is useful for the development of food and health products with immune regulation functions. and drugs are of great importance.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide KQSLPPGLAVKDLK and a preparation method and application thereof. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anticancer, blood pressure lowering, antibacterial, cholesterol lowering, antidiabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] At present, the research on bioactive peptides mostly focuses on food-derived peptides, and there are few studies and reports on non-food-derived peptides. And research results have confirmed that, compared wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉栾媛媛王娟张伯宇占文静谢雨佳李政郭婷婷
Owner SHANGHAI JIAO TONG UNIV
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