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Bioactive peptide KQSLPPGLAVKDLK as well as preparation method and application thereof

A bioactive peptide, active technology, applied in the field of protein, to achieve the effect of improved ability, improved quality, good anti-inflammatory activity

Active Publication Date: 2021-03-12
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of 60S ribosomal protein L38 protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide KQSLPPGLAVKDLK as well as preparation method and application thereof
  • Bioactive peptide KQSLPPGLAVKDLK as well as preparation method and application thereof
  • Bioactive peptide KQSLPPGLAVKDLK as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The artificial synthesis of embodiment 1 bioactive peptide KQSLPPGLAVKDLK

[0030] 1. Synthesis of Bioactive Peptides

[0031] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0032] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0033] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0034] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0035] 5. ...

Embodiment 2

[0072] The immune activity experiment of embodiment 2 bioactive peptides

[0073] 1. MTT method to determine the in vitro macrophage proliferation ability of bioactive peptide KQSLPPGLAVKDLK

[0074] 1. Experimental reagents and instruments

[0075] Reagent: experimental animal balb / c mouse (male 6-8 weeks old) Animal Experiment Center, College of Agriculture and Biology, Shanghai Jiaotong University; mouse spleen lymphocyte-derived bioactive peptide KQSLPPGLAVKDLK obtained in Example 1; 3-(4,5 -Dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) Amresco; LPS (lipopolysaccharide) Sigma; Bovine Serum Albumin (BSA) Genebase; Triple The solution is an aqueous solution containing 10% SDS, 5% isobutanol and 0.012mol / L HCl.

[0076] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus; Dragon WellscanMK3 microplate reader...

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Abstract

The invention relates to the field of protein, in particular to a bioactive peptide KQSLPPGLAVKDLK as well as a preparation method and application thereof, and the amino acid sequence of the bioactivepeptide KQSLPPGLAVKDLK is Lys-Gln-Ser-Leu-Pro-Pro-Gly-Leu-Ala-Val-Lys-Asp-Leu-Lys. In-vitro immune function regulation experiments prove that the bioactive peptide KQSLPPGLAVKDLK has a very good immune regulation function. The bioactive peptide KQSLPPGLAVKDLK disclosed by the invention has the effects of enhancing the in-vitro proliferation capability of macrophages and lymphocytes, improving thecapability of resisting external pathogen infection of an organism, reducing the morbidity of the organism and improving the quality of life, and has very important significance for developing foods,health care products and medicines with an immunoregulation function.

Description

technical field [0001] The present invention relates to the protein field, in particular to a biologically active peptide KQSLPPGLAVKDLK and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results that, co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉栾媛媛王娟张伯宇占文静谢雨佳李政郭婷婷
Owner SHANGHAI JIAO TONG UNIV
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