Dominant molecular marks cosegregation with tuberculate fruit (Tu) gene in cucumber

Abstract

The invention provides two dominant molecular marks cosegregation with tuberculate fruit (Tu) gene in cucumbers, wherein the first dominant molecular mark is composed of 236 nucleotides shown in the sequence table SEQ ID NO.1; and the second dominant molecular mark is composed of 468 nucleotides shown in the sequence table SEQ ID NO.2. Both the two dominant molecular marks disclosed by the invention exist in the cDNA areas of the tuberculate fruit (Tu) shown in the sequence table SEQ ID NO.7 and have high stability, and can be applied to the screening of cucumber types (except for the mutant variety g1 without thorns and tubercles) with / without tubercles around the world simply and rapidly and in high throughput. According to the cDNA areas of the tuberculate fruit (Tu) shown in the sequence table SEQ ID NO.7, a plurality of molecular marks can be designed as the dominant molecular marks for the screening of cucumber types (except for the mutant variety g1 without thorns and tubercles) with / without tubercles around the world, and the cDNA areas of the tuberculate fruit (Tu) can lay the molecular foundation for the clone of the glabrous gene and the gloss gene of cucumbers.

Description

technical field [0001] The invention relates to molecular markers in the technical field of genetic engineering, in particular to two dominant molecular markers co-separated with cucumber fruit tumor gene Tu. Background technique [0002] Cucumber (Cucumis sativus L.) is an annual herbaceous plant belonging to the genus Cucumis of Cucurbitaceae. Cucumber is one of the ten most important vegetable crops in the world and one of the main vegetable crops in my country. [0003] Fruit is the most important part of cucumber's economic traits. Cucumber fruit belongs to gourd, which is jointly developed by ovary and receptacle. An important character on cucumber fruit is fruit tumor, which is a tumor-like protrusion on the surface of cucumber fruit. The study of tuberculate genes began in 1913. Tuberculate is a wild trait of cucumber, and tuberculate traits are regulated by Tu (Tuberculate fruit) gene. Tuberculate (Tu) is dominant and no tuberculate (Tu) is recessive. sex. On the...

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Problems solved by technology

[0004] In 2009, Zhang Weiwei and others concluded that the fruit tumor trait of cucumber belongs to a single-gene dominant trait, using 247 F 2 The group initially located the fruit tumor gene between the fifth chromosome SSR marker 16203 and the SCAR marker C_SC933, and the genetic distances were 1.3cM ​​and 5.9cM respectively. For detailed results, please refer to: Zhang W W et al. in "Theoretical and Applied Genetics" (Theoretical and Applied Genetics) Science) published in 2009, volume 120, phase 3, pages 645-654, entitled "Identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.)" (cucumber fruit tumor gene tightly linked molecular Marker Preliminary Positioning) article, the above-mentioned markers still have problems such as relatively small analysis population and relatively long linkage distance. There are more than 100 candidate genes in the candidate interval, and there is no analysis of the candidate genes.

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