Dominance molecular marker for controlling coseparation of cucumber genes Tril with or without fruit thorns

Abstract

The invention provides a dominance molecular marker for controlling coseparation of cucumber genes Tril with or without fruit thorns. The dominance molecular marker is named as CoT-01, a nucleotide sequence of the CoT-01 is shown in SEQ ID NO.1, the CoT-01 is linked to the gene Tril with fruit thorns, and the gene tril without fruit thorns cannot amplify specific fragments. Coseparation of the molecular gene and the gene Tril loci is achieved, and great help is provided for distinguishing cucumber microtrichia and glabrous strains; the dominance molecular marker has the high stability and can easily, conveniently and rapidly distinguish phenotypes with or without fruit thorns in the cucumber seedling period, and can be applied to related molecular marker assistant breeding.

Description

technical field [0001] The invention relates to molecular markers in the technical field of genetic engineering, in particular to a dominant molecular marker co-segregated with the gene Tril controlling the presence or absence of cucumber fruit thorns. Background technique [0002] Cucumber (Cucumissativus L.) is an annual herbaceous plant belonging to the genus Cucumis of Cucurbitaceae. As one of the ten most important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country. Fruit is the most important part of cucumber's economic traits. Cucumber fruit belongs to gourd, which is jointly developed by ovary and receptacle. Fruit thorns are highly specialized morphology of epidermal hairs on fruits, and are also important fruit traits. The presence or absence of fruit thorns is regulated by the Trichome-less (Tril) gene, the wild character (Tril) with fruit thorns is dominant, and the mutant without fruit thorns (tril) is recessive. The r...

AI Technical Summary

Problems solved by technology

The adult plants of the tril mutant without fruit thorns and the adult plants of the microhair mutant gl have no visible hairs on the epidermis and no visible fruit spines, which are difficult to distinguish with the naked eye, and no further location and analysis of the candidate genes of Tril have been carried out by the predecessors.

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