Molecular marker Marker Sex 1 and identification method for quickly identifying genetic sex of portunus trituberculatus

A technology for molecular markers of Portunus trituratus, which is applied in the directions of biochemical equipment and methods, DNA/RNA fragments, and microorganism determination/inspection, etc., can solve the problems such as gender molecular markers of Portunus trituratus that have not yet been seen, and achieves high accuracy, Simple and fast operation, low sample requirements

Active Publication Date: 2018-10-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
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Problems solved by technology

However, there is no report on sex molecular markers in Portunus trituberculatus

Method used

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  • Molecular marker Marker Sex 1 and identification method for quickly identifying genetic sex of portunus trituberculatus
  • Molecular marker Marker Sex 1 and identification method for quickly identifying genetic sex of portunus trituberculatus

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Embodiment 1

[0023] 1) DNA extraction

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Abstract

The invention provides a molecular marker Marker Sex 1 and an identification method for quickly identifying genetic sex of portunus trituberculatus, wherein the Marker Sex 1 has the nucleotide sequence represented as the SEQ ID No.1. The identification method includes steps of: extracting genome DNA from muscle tissue of the portunus trituberculatus; with the genome DNA as a template, performing aPCR amplification reaction; purifying and sequencing the PCR amplification product; comparing the sequencing result with the DNA sequence of the molecular marker, and determining male if double peakexists or female if single peak exists. The identification method is simple and quick in operation and is low in demand on the sample, has high identification accuracy, is free of influence due to tissue specificity and environment, and has important effect for establishing all-female or high-female-ratio larval breeding technologies.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal biology, and in particular relates to a molecular marker Marker Sex 1 for rapidly identifying the genetic sex of Portunus trituberculatus and an identification method. Background technique [0002] Portunus trituberculatus ( Portunus trituberculatus ) belongs to Crustacea, Decapoda, Portunidae, and Portunus. It is an important fishery catch and mariculture object in my country. It has fast growth, tender meat, delicious taste, and nutrition Rich and other advantages, it is an important seafood with high economic value, and the market demand continues to increase. The economic value of Portunus trituberculatus female crabs is significantly higher than that of male crabs. Therefore, in order to improve the breeding efficiency, it is urgent to break through the breeding and identification methods of all-female or high-female ratio seedlings of Portunus trituberculatus. One of the key technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6879C12N15/11
CPCC12Q1/6879
Inventor 吕建建刘萍李健高保全任宪云
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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