SCAR molecular marker and identification method for identifying banana fusarium wilt resistance

A banana fusarium wilt and molecular marker technology, applied in the field of plant molecular biology, can solve problems such as complex disease resistance mechanism of fusarium wilt, and achieve the effects of short cycle, simple operation and good repeatability

Active Publication Date: 2016-11-16
东莞市香蕉蔬菜研究所
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Problems solved by technology

The disease resistance mechanism of banana Fusarium wilt is relatively complex, and there are many genes related to disease resistance. At present, there are still relatively few molecular methods that can be directly and effectively used in banana breeding. Therefore, it is urgent to speed up the development of banana disease-resistant biological breeding technology

Method used

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  • SCAR molecular marker and identification method for identifying banana fusarium wilt resistance
  • SCAR molecular marker and identification method for identifying banana fusarium wilt resistance
  • SCAR molecular marker and identification method for identifying banana fusarium wilt resistance

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[0030] specific implementation

[0031] The present invention is further illustrated and defined by the following examples without limitation. Through examples, scientific researchers can have a clearer understanding of the present invention, and can make certain changes and modifications to the present invention on this basis to obtain different research effects. The experimental methods in the following examples are conventional methods unless otherwise specified. The reagents involved in the experiment are all conventional reagents, and they are used according to the product instruction manual.

[0032] 1. DNA extraction from banana leaves

[0033] Banana (genotype AAA) varieties (Table 1) with different resistance to banana wilt were selected for DNA extraction.

[0034] The DNA extraction method adopts the modified CTAB method, and the specific operation is as follows:

[0035] 1. For the DNA extraction of bananas, use the leaves of bananas, select freshly extracted y...

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Abstract

The invention belongs to the technical field of plant molecular biology and particularly relates to an SCAR molecular marker and an identification method for identifying banana fusarium wilt resistance. By means of the SCAR molecular marking technology, a special strip of banana fusarium wilt susceptible varieties is screened out, a specificity primer is designed according to the sequence of the strip for specificity amplification, and the primer is converted into the SCAR molecular marker. The SCAR molecular marker and the method are used for identifying banana fusarium wilt resistance of banana varieties, provide a molecular assisting technology for banana disease-resistant new variety breeding, and can be further used for auxiliary screening of new banana varieties (strains) with banana tissue culture seedlings, banana field variant strains, chemically induced progenies and the like as screening populations.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and in particular relates to a SCAR molecular marker for identifying banana fusarium wilt resistance and an identification method thereof. Background technique [0002] Banana is the fourth food crop in the world after rice, wheat, and corn. It is also the second largest fruit after citrus, and it is the fresh fruit with the largest fruit trade volume in the world. Banana Fusarium wilt is a devastating disease of banana and also an important quarantine disease. Since 1967, after my country first discovered race 4 of banana wilt in Taiwan, it has spread to Australia, Africa and some Asian countries, causing widespread concern at home and abroad. Fusarium wilt has also been found in major banana producing areas such as Guangdong, Guangxi, Fujian, Hainan, and Yunnan in China, and has become a major obstacle to the development of the banana industry (You Chunping, 2008, Journal of Hu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 王芳夏玲吕顺牛玉清曾莉莎周建坤
Owner 东莞市香蕉蔬菜研究所
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