Method for constructing China asparagus bean genetic resource database based on ISSR molecular marker and uses thereof

Abstract

The invention discloses an ISSR molecular marking based method for constructing a Chinese asparagus bean generic resource database and application thereof. The invention also utilizes the data base to identify the trueness of a variety; according to field forms, the consistency and typicality among individual plants of the variety are identified, separated and atypical materials are given up, and DNA of the individual plant of the consistent and typical variety is picked up; by utilizing three ISSR markings with high polymorphism, the consistency of DNA level among the individual plants is primarily selected, and the variety and individual plant constructing a fingerprint are finally determined; and by utilizing the finally selected variety and individual plant, the ISSR analysis of the Chinese asparagus bean is made to construct a fingerprint database. The invention overcomes the defects existing in the construction of the prior generic resource database, such as separation among different individual plants of the variety and impurity on the DNA level, the reliability and accuracy of the Chinese asparagus bean ISSR fingerprint data base constructed by the method are greatly improved, and the planting varieties of the Chinese asparagus bean can be effectively and conveniently identified and distinguished.

Description

technical field [0001] The invention belongs to the establishment and application of a plant variety genetic resource database, in particular to a method for identifying a Chinese long cowpea genetic resource database based on ISSR molecular markers and the application of the database. Background technique [0002] Cowpea (Vigna unguiculata L.Walp.) is an important leguminous plant with a global planting area of ​​more than 125 million hectares and an annual output of more than 3 million tons. Among them, long cowpea (V. sesquipedalis) is the most economically important subspecies of cowpea. It can be used as vegetables, grains, and animal feed, so it is widely planted in Southeast Asia and China. China is the center of secondary origin of long cowpea and has a long history of cultivation. China has a vast territory and complex ecological environment, and long cowpea is planted all over the country, so a wealth of long cowpea local varieties have been formed. However, so f...

AI Technical Summary

Problems solved by technology

SSR marker primers are specific in sequence, highly polymorphic and co-dominant, and are the most widely used in the identification of resources and seed purity. However, there are only more than 20 effective SSR markers that are different in the entire genus Vigna. The genetic relationship between species within a species is closer, so there are fewer effective SSR markers, which is far from meeting the needs of practical applications

RAPD markers detect many sites and common primers, but it is generally considered not stable enough, and RAPD markers in cowpea cannot reflect blood relationship, so it is considered unsuitable for cowpea kinship analysis

The polymorphism of AFLP and SAMPL markers is very high, which can effectively distinguish different varieties of common cowpea (V. unguiculata). However, the procedures of enzyme digestion, amplification and PAGE detection are complicated and costly. Or resource identification and other applied research is rarely used

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