Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis

A soybean cyst nematode, tea saponin technology, applied in the application, nematicide, chemicals for biological control, etc., can solve the problem of no tea saponin on soybean cyst nematode disease research reports and patent applications, etc. problem, to achieve the effect of easy degradation

Active Publication Date: 2014-10-01
SOUTH CHINA AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis
  • Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis
  • Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Determination of the inhibitory activity of tea saponin on egg hatching of soybean cyst nematode

[0020] Pick mature soybean cyst nematodes with consistent color and size under anatomical environment, disinfect with 1% sodium hypochlorite for 3-5 minutes, rinse with sterile water several times, put them into 24-well plates, and add 10 mature soybean cyst nematodes to each treatment Soybean cyst nematode, add 1mL of tea saponin solution for test, the test solution is prepared with sterilized water, each treatment is repeated 4 times, with sterilized water as control, cultivated at 25°C, and observed at 3d, 5d, and 10d respectively Record the number of nematodes hatched, and calculate the inhibition rate of nematode egg hatching according to the following formula.

[0021]

[0022] The results showed that the tea saponin at 2mg / mL, 5mg / mL and 8mg / mL concentrations was treated for 3 days, and the egg hatching inhibition rates of soybean cyst nematode were 43...

Embodiment 2

[0027] Example 2: Determination of the poisonous activity of tea saponin on soybean cyst nematode second instar larvae

[0028] Using liquid soaking method: add 1mL of the test solution in the 24-well plate, the test solution is prepared with sterilized water, then add 30-50 nematodes for the test, put them in a 25°C incubator, and wait for 24h, 48h, Check and record the number of live nematodes and dead nematodes under an inverted microscope at 72 hours, and calculate the respective corrected mortality rates according to formula 3. And, resuscitate the treated nematodes for 72 hours for 24 hours, check and record the number of live nematodes and dead nematodes under an inverted microscope again, and calculate the corrected mortality rate after 24 hours of resuscitation according to the following formula. Each treatment was repeated 4 times, and sterile water was used as control.

[0029]

[0030] The results showed that the corrected mortality rates were 29.86%, 59.92% an...

Embodiment 3

[0036] From May to June 2013, in the field of soybean cyst nematode infection in the Langfang base of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, a field trial of tea saponin in the control of soybean cyst nematode was carried out. The area of ​​the plot is 15m 2 .

[0037] Set three concentrations of tea saponin 120g, 240g, and 300g per plot to treat, the control drug is 10% thiazolphos granule 50g per plot, set no drug application blank control (CK), each treatment is repeated 3 times, and arranged randomly. On May 17th, rake the ground evenly and raise the ground, 15m 2In a small plot, 4 small ditches were opened in the plot, and the treatment agent was evenly spread in the ditch, and then the soybean seeds were sown in the ditch where the chemical was applied, and finally covered with soil and stepped on, and watered the next day. One month later, on June 20, a sampling survey was carried out, 10 plants were randomly selected from each plo...

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Abstract

The invention relates to application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis, and belongs to the field of biological prevention and treatment. The tea saponin serves as a main effective component, is high in toxic activity to heterodera glycines, and can be effectively used for preventing and treating the soybean sporocyst nematodiasis.

Description

technical field [0001] The invention relates to the field of biological control, in particular to the application of tea saponin in preventing and controlling soybean cyst nematode diseases. Background technique [0002] Plant nematodes are one of the important pathogenic organisms of plants. With the transfer of farmland, the development of modern agriculture towards industrialization and bases, the long-term large-scale planting of single crops, the continuous cropping all year round and the improvement of land reuse rate, the number of nematodes in the soil is rapidly increasing. Cumulatively, the problem of nematode diseases has become increasingly prominent. Wheat cyst nematodes (Heterodera.avenae, Heterodera.filipjevi) and soybean cyst nematodes (Heterodera glycines) in China are common and harmful, causing great losses and impacts on agricultural production in my country (Peng Deliang et al., 1993, 2008, 2012; Feng Zhixin , 2001). Cyst nematodes are a type of parasit...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P5/00
Inventor 文艳华彭德良廖金铃黄文坤
Owner SOUTH CHINA AGRI UNIV
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