52 results about "Nematode infection" patented technology

The present invention provides isolated nucleic acids encoding polypeptides with chitinase activity and methods for using the nucleic acids to enhance resistance of plants to fungal and nematode infections.

The present invention provides compositions with chitinase activity and methods for using such compositions to enhance resistance of plants to fungal and nematode infections.

The invention provides compositions having Bacillus thuringiensis Crystal (Cry) proteins including transgenic plants expressing those proteins, and methods of using those proteins to prevent, inhibit or treat parasitic infections in plants and vertebrates.

Genes whose expressions are up-regulated or down-regulated in plant roots in response to nematode infection are disclosed. These genes may play an important role in plant defense against SCN infection. Several nematode-inducible promoters have also been identified in plants which ma be used in nematode inducible gene expression construct. Expression of these SCN responsive genes may be manipulated to obtain SCN resistant lines.

The invention provides liposomal-attractant formulations comprising pesticides or nematicides for control of pests. The formulations can be applied to pre- or post-emergent crops and to soil, plant media, plants, plant tissues and seeds. The liposomal-attractant formulations are also useful to treat or control pest or nematode infections of humans and animals.

The present invention presents methods to alter the genetic composition of crop plants susceptible to nematode infection to improve tolerance to the same. Methods and compositions for modulating key pathways involved in the syncytial event of nematode infection and for preventing the cascade of differential gene expression caused by the same as disclosed. Applicants have found that the microRNA miR396 acts as a master switch of syncytial gene expression changes in plants after infection, and further that miR396 and certain growth regulating transcription factors (GRF) are connected through feedback interaction in syncytium initiation and maintenance.

The invention relates to an antigen used for the early diagnosis of haemonchus contortus infection, and application thereof. The invention authenticates an antigen used for parasite infection, and theamino acid sequence of the antigen is disclosed in SEQ ID NO.1. The antigen can be used for carrying out specific recognition on an antibody in the serum of a host infected with the haemonchus contortus for 14 days, a definite diagnosis is made before 4-6 days of ovum ovulation, and the pollution of the ovum for the environment and a pasture can be avoided so as to perform an important meaning for the prevention and control of the haemonchus contortus disease. By use of the antigen disclosed by the invention, the antigen is used for establishing an indirect ELISA (Enzyme Linked ImmunosorbentAssay) detection method, and therefore, the antigen has the advantages of being high in sensitivity, good in specificity, simple in operation, high in diagnosis speed, economic, convenient and the like during large-scale detection, and has a good application prospect in the diagnosis, the prevention and the curing of the haemonchus contortus disease of ruminants, including cows, goats, deer, camels and the like.

The invention discloses a new use of chloroquine in prevention and control of root-knot nematodes, and belongs to the technical field of the prevention and control of root-knot nematodes. The invention relates to a use of chloroquine in prevention and control of root-knot nematodes and a use of chloroquine in inhibition of infection, development and / or oviposition of root-knot nematodes, wherein the chloroquine is chloroquine phosphate, hydroxychloroquine sulfate or quinine sulfate. The invention also relates to a use of chloroquine in preparation of a root-knot nematode pesticide or a root-knot nematode infection, development and / or oviposition inhibitor, wherein the active component of the root-knot nematode pesticide or the root-knot nematode infection, development and / or oviposition inhibitor is chloroquine phosphate, hydroxychloroquine sulfate or quinine sulfate; and the root-knot nematode pesticide or the root-knot nematode infection, development and / or oviposition inhibitor is an aqueous solution of chloroquine phosphate, hydroxychloroquine sulfate or quinine sulfate. According to the present invention, the prevention and control of root-knot nematodes with the aqueous solution of chloroquine are safe, non-toxic and effective.

The invention discloses an amino acid water-soluble fertilizer. The water-soluble fertilizer is prepared from paecilomyces lilacinus fermentation liquor, polyglutamic acid fermentation liquor, micromolecular amino acid, chitosan oligosaccharide, potassium alginate, chelated medium elements and a thickening agent. The product has the advantages that the fermentation liquor is directly used, so thatthe centrifuging or filtering process is avoided, and the cost is reduced; paecilomyces lilacinus is relatively good in nematode control effect, safe and free of pollution. The potash fertilizer increases the toughness of crop roots and can effectively prevent nematode infection; the micromolecular amino acid can be directly absorbed and utilized by the root system to promote rapid growth of thecrop root system or rapid recovery of the damaged root system; chitosan oligosaccharide is matched with organic matters, so that the influence of root-knot nematodes on crops can be reduced, and combined infection of bacteria or fungi on plant root systems is reduced. The microbial agent and the water-soluble fertilizer raw material can be mutually dissolved after being mixed, the solution is in astable colloid state, and conidia of the microbial agent can stably survive and keep biological activity for a long time.

The invention provides transgenic plants that exhibit increased resistance to nematode infection by virtue of overexpression of a gene that encodes trehalase in nematode-induced syncytia. Expression vectors comprising trehalase-encoding polynucleotides and methods of employing such vectors to increase nematode resistance of plants are also provided.

The present invention provides expression vectors encoding double stranded RNAs that target certain plant genes required for maintenance of parasitic nematode infection, nematode-resistant transgenic plants that express such double-stranded RNAs, and methods associated therewith. The targeted plant gene is a GLABRA-like gene, a homeodomain-like gene, a trehalose-6-phosphate phosphatase-like gene, an unknown gene having at least 80% homology to SEQ ID NO:16, a ringH2 finger-like gene, a zinc finger-like gene, or a MIOX-like gene.

The present application relates to the treatment of nematode infections. For example, the application relates to the use of compounds of Formula I and / or II as defined herein for treatment of a nematode infection or a disease, disorder or condition arising from a nematode infection:

The invention belongs to the field of agricultural plant protection and particularly relates to a signaling protein composition for controlling root-knot nematodes and a preparation method and application of the signaling protein composition. The composition comprises an active component A, namely signaling protein, an active component B, namely biocontrol spore powder and an active component C, namely non-fumigating nematicide. According to the root-knot nematode controlling method, biological control is used as a main means, and chemical control is used as an auxiliary means; by improving the crop stress resistance and the crop growing vitality, weakening the root-knot nematode infection capability and the after-infection fixed feeding site generation capability and the like, the purposeof controlling the root-knot nematodes through a united function is achieved; the components of the composition show an obvious synergetic effect within a certain matching range, the control effect is remarkably improved compared with separate use of the contained components, the dosage is reduced, and possible residues of a small quantity of synthetic chemical substances in the ecological environment are reduced.

The present invention relates to vaccines comprising a ShK domain of a filarial nematode protein. These vaccines may be used for the prevention and / or treatment of filarial nematode infections. The invention also relates to novel proteins comprising a ShK domain of a filarial nematode protein and pharmaceutical compositions. The invention may be used for the prevention and / or treatment of filarial nematode infections in canine subjects, and also in human subjects.

The invention discloses application of an autophagy gene ATGs of tomatoes in improvement on root-knot nematode resistance of plants. The application comprises the steps: (1) constructing a root-canceragrobacterium tumefaciens engineering strain A containing a tomato ATG10 gene overexpression vector; (2) mediating the root-cancer agrobacterium tumefaciens engineering strain A to convert an explantof a target plant, so as to obtain an ATGs-gene-overexpressed plant; and (3) subjecting the transgenic plant to root-knot-nematode-inoculated stress treatment, and observing autophagy changes, a root-knot quantity and a phenotype. Researches of the invention discover that Southern root-knot nematode infections can rapidly induce expression of autophagy genes in tomato root systems, the sensitivity to the root-knot nematodes is improved by mutants, and the sensitivity is lowered due to ATG10 overexpression. Namely, autophagy plays a role in root-knot nematode resistance of the tomatoes.

The present invention relates to an isolated monoclonal antibody mAb PAB-1, deposited at ATCC on 24 January 2002 and accorded accession PTA-4005, which binds to a surface antigen on nematode L3. It also relates to the antigen which binds to the monoclonal antibody and uses for the monoclonal antibody and antigen in diagnosing and treating or preventing nematode infection.

The invention provides a method of identifying anti-nematode compounds and further provides transgenic nematodes that may be used to practice the method. In particular, the invention provides a screen for compounds that inhibit a nematode secretion pathway e.g, compounds that inhibit the secretion of proteins by nematodes. The transgenic nematodes express reporters for nematode secreted proteins. In preferred embodiments of the invention the screen is performed using C. elegans, i.e., certain embodiments of the invention utilize C. elegans and C. elegans secretory pathways as a model system for parasitic nematodes and parasitic nematode secretion pathways. The invention also provides pharmaceutical compositions that may be used in the treatment and prevention of nematode infection in humans and animals and anti-nematode agents that may be used to protect plants from plant-parasitic nematodes. In addition, the invention provides a genetic screen for identifying additional targets for anti-nematode compounds.

The invention discloses a nematode preparation for preventing and controlling flower slugs as well as a preparation method and application thereof, belonging to the technical field of biopesticides. The nematode preparation comprises a preparation A and a preparation B which contain different active ingredients. The active ingredients of the preparation A comprise parasitic Rhabditis axei and a substrate inhibitor L-dopa of polyphenol oxidase; and the active ingredients of the preparation B comprise quercetin which is a structure inhibitor of polyphenol oxidase. When the nematode preparation is used for preventing and controlling the flower slugs, the preparation A is applied to the surface of the rhizosphere soil of the flowers at nightfall; and the preparation B is sprayed on tender shoots and leaves of flowers in the afternoon of the same day. According to the invention, the slug biocontrol factor parasitic Rhabditis axei and two functional inhibitors for polyphenol oxidase of the slugs are combined for use for the first time, the field fast-acting property of the parasitic Rhabditis axei is greatly improved by utilizing feeding habit attraction of the slugs; and different application positions, application time and methods are selected for the preparation A and the preparation B, so nematode infection and killing of the slugs are accelerated.

The present invention provides a recombinant prokaryotic expression protein antigen for detecting autochthonous trichinella spiralis antibody and its preparation method. Said product can be used as detection antigen of indirect-ELISA and Dot-ELISA method for detecting autochthonous trichinella spiralis antibody, also can be used as gene engineering subunit vaccine for preventing autochthonous trichinella spiralis infection. The preparation process of said product includes the following steps: (1) designing specific primer for amplifying TNPG gene; (2), utilizing PCR amplification to obtain TNPG gene segment; (3), cloning TNPG gene and sequencing, and making identification; (4), directionally subcloning TNPG gene to pET-3a prokaryotic expression vector; (5), induced expression of TNPG gene in colibacillus; and (6), affinity chromatography purification of TNPG gene expression protein.

The invention provides transgenic plants that exhibit increased resistance to nematode infection by virtue of overexpression of a gene that encodes trehalase in nematode-induced syncytia. Expression vectors comprising trehalase-encoding polynucleotides and methods of employing such vectors to increase nematode resistance of plants are also provided.

The invention provides a construction method and application of a transgenic plant with interaction of a cucumber root system and meloidogyne incognita, and belongs to the technical field of plant genetic engineering and plant protection. According to the method disclosed by the invention, in the period of relatively vigorous division and differentiation of cucumber seedlings with unexpanded cucumber cotyledons, a bacterial solution of agrobacterium rhizogenes transformed with a target gene vector to be inspected or knocked out of the target gene vector is inoculated, so that a hairy root system is generated, and an efficient transgenic root system is established and used for nematode infection and research on interaction between root systems. The method provided by the invention can solve the problem that the response of phenotypes and root systems to nematode infection is deviated due to the fact that the change of overground part target genes caused by a whole-plant transgenic system possibly affects the change of the root systems, and screening and functional verification of root system related nematode-resistant genes are more accurately carried out.

The invention discloses a sweet potato stem nematode resistance related protein IbPIF1 as well as a coding gene and application thereof. The IbPIF1 is a protein as shown in a1) or a2) or a3) or a4): a1) a protein as shown in a sequence 2; a2) a fusion protein obtained by connecting a tag to the N terminal or / and C terminal of the protein as shown in the sequence 2; a3) a stem nematode disease resistance related protein obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in the sequence 2; a4) a protein which has 90% identity with the amino acid sequence shown in the sequence 2, is derived from sweet potatoes and is related to stem nematode disease resistance. The coding gene of the IbPIF1 protein is introduced into the sweet potato to obtain a transgenic plant, and the transgenic plant is subjected to stem nematode infection to find that the resistance of the transgenic plant is enhanced. It is shown that the IbPIF1 protein plays an important role in the plant disease resistance process and has a wide application prospect.

A diagnostically useful carrier includes (a) a peptide including the amino acid sequence set forth in SEQ ID NO:1 or a variant thereof, and (b) a somatic lysate of Toxocara canis larvae. Further, a kit, use, methods, and compositions that include the diagnostically useful carrier are disclosed.