13 results about "Nematode infection" patented technology

The application belongs to the technical field of plant disease prevention and treatment, and relates to a determination method for visually and rapidly evaluating the effect of pesticides on inhibition of soil-borne pathogen infection on crop root systems. Agar powder is added to deionized water and boiled until dissolved, sterilized by high-pressure steam and cooled to 60-65 DEG C to obtain an agar culture medium, and the agar culture medium is poured into a culture dish and continuously cooled to solidification. A double-layer filter paper is laid on the solidified culture medium, pre-germinated seeds are placed above the filter paper and cultivated until the root system expands. After the root system of the seedling expands, the pathogen is inoculated and pesticide liquid is applied; and the activity of the pesticide on the pathogen is evaluated. The advantages of the application are that rapid inoculation can be realized, symptoms can be observed in real time, nematodes can be rapidly inoculated and dyed, pesticides can be applied at different stages before, during and after pathogen infection, and the protective and therapeutic activities of the pesticide can be rapidly evaluated. The application greatly facilitates the related research on pathogen or nematode infection, and provides a rapid and efficient method for evaluating the activity of the pesticide on the soil-borne pathogen.

The invention relates to the technical field of green prevention and control of plant diseases, in particular to application of a cucumber-marigold intercropping mode to prevention and control of cucumber root knot nematode diseases. Cucumbers and marigold are intercropped and cultivated, and cucumber root-knot nematode infection and / or reproduction are / is inhibited by the allelopathy of the marigold; wherein marigold plants are planted in soil and / or a culture medium, so that stable root systems are formed before cucumber planting; 7-21 days after field planting of the marigold, cucumber seedlings are planted in the same cultivation container or the adjacent rhizosphere space, the root system of the cucumber and the root system of the marigold are located in the same cultivation environment but not directly twined, and the marigold keeps surviving and growing in the early stage and the middle stage of cucumber growth. The method solves the problem that an existing intercropping mode lacks a system method with a cultivation structure and a planting time sequence as the core, and has the advantages that operation is easy and convenient, applicability is high, popularization is easy, compared with cucumber single cropping, the root knot index of cucumbers under an intercropping system is remarkably reduced, and the requirement for green agriculture development is met.

The present application relates to the field of pine wood nematode disease identification, in particular to the present application, through the investigation of soil relative humidity, light intensity, inoculation amount, environmental temperature, seedling strain, seedling age and other related indexes, the conditions of the most susceptible to nematode infection of pinus massoniana under natural conditions are obtained, the method of grading pinus massoniana by disease death frequency is summarized, in addition, the research group continuously optimizes the conditions of infection under natural environment, and prepares nematode active liquid without damage to plants by using plant liquid, it is verified that after using the nematode active liquid, the disease time of pinus massoniana seedling stage can be greatly shortened, through optimizing the active liquid, the method of grading the disease of pinus massoniana by judging the disease time during the inoculation of nematode in pinus massoniana seedling stage is given, which greatly shortens the grading determination time of pinus massoniana.

Disclosed are methods for increasing infectivity of entomopathogenic nematodes in any target area for the purposes of insect control, said method comprising:(a) mixing an aqueous nematode infectivity increasing composition with (i) entomopathogenic nematodes to activate said entomopathogenic nematodes for increased infectivity prior to application of said entomopathogenic nematodes to said target area, or (ii) seeds to produce coated seeds, and(b) applying said entomopathogenic nematodes to said target area or planting said coated seeds in said target area;wherein said aqueous nematode infectivity increasing composition is produced by methods disclosed herein.

The present invention relates to recombinant activation-associated secretory protein (ASP) or a fragment thereof, wherein the ASP or fragment comprises an N-glycan containing core α1,3-fucose and / or core α1,6-fucose (Fuc). The present invention further relates to a pharmaceutical composition comprising such recombinant ASP or a fragment thereof. Furthermore, the present invention relates to recombinant ASP or a fragment thereof for use as a human or veterinary drug, particularly as a vaccine, more specifically for use against parasitic nematode infections.

A pine wood nematode infection point collecting device belongs to the technical field of nematode collection and comprises a pine wood body and further comprises an upper shell and a lower shell, the upper shell and the lower shell form a shell with an inner cavity communicated, the width of the inner cavity of the upper shell is larger than that of the inner cavity of the lower shell, and the right side wall of the upper shell and the right side wall of the lower shell are located on the same vertical line. An edge with a blade is arranged on the left side of the top end of the upper shell, fixing pins are installed on the front side wall and the rear side wall of the lower shell respectively, and a frame is arranged on the right side of the upper shell and arranged on the outer sides of the upper shell and the lower shell. According to the utility model, complete and representative sheet-shaped tree samples can be extracted, the original natural distribution state of nematodes at pine wood cannot be destroyed, the infection condition of the nematodes in trees can be more comprehensively and accurately analyzed, the distribution integrity and authenticity of the nematodes in the trees during detection are effectively maintained, and the detection accuracy is improved. The upper shell does not need to be manually pushed for sampling, and compared with manual handheld equipment for sampling, sampling is more accurate, and operation is convenient.

The utility model provides a bursaphelenchus xylophilus infectious disease detector based on a Raman scattering spectrum analysis technology, and particularly belongs to the technical field of infectious disease detection. A Raman spectrum imaging module, a data acquisition module, a data comparison module and a result output module form a core module of the device, so that light path design and a CNN algorithm are optimized, the signal-to-noise ratio of the device is improved by 30%, the classification accuracy rate reaches 97.2%, trace metabolite change in the early stage of infection can be detected, the infection incubation period is shorter than 7 days, and the detection accuracy is improved. Therefore, the device has the characteristics of high sensitivity and specificity. The device comprises a laser fixing support and a beam splitter prism assembly, and further comprises a light filtering module and a sample bin. Four-corner buckles are arranged on the inner side of the laser fixing support, the laser fixing support is used for installing the laser through the buckles, the beam splitter prism assembly is installed on one side of the laser, the light filtering module is installed on the other side of the beam splitter prism assembly, and the sample bin is installed on the other side of the light filtering module.

This invention relates to the fields of molecular biology and genetic engineering, specifically to the application of nematode nicotinamide genes in regulating the resistance of soybean cyst nematode infection. Three candidate nicotinamide genes from soybean cyst nematodes were screened through structural alignment. Hetgly07149, Hetgly05004, Hetgly05016 The study found that the nicotinamide gene plays a positive regulatory role in nematode infection of plants. After silencing this gene using host-induced gene silencing (HIGS) technology, the infection ability of soybean cyst nematodes in both the susceptible variety Wm82 and the resistant variety Forrest soybean was significantly reduced. The proportion of nematodes in late developmental stages (J3 and J4) and the total number of nematodes invading the host plant were also significantly decreased. This provides an important target gene and theoretical basis for developing novel transgenic crops resistant to soybean cyst nematodes using HIGS technology, which is of great significance for protecting food security for crops such as soybeans.

The present invention relates to a recombinant activation-associated secreted protein (ASP) or fragment thereof, said ASP or fragment comprising an N-glycan comprising a core α1,3-fucose and / or a core α1,6-fucose (Fuc). The invention further relates to a pharmaceutical composition comprising such a recombinant ASP or fragment thereof. Additionally, the invention relates to the recombinant ASP or fragment thereof for use as a human or veterinary medicine, in particular as a vaccine, more in particular for use against parasitic nematode infections.

The application belongs to the field of biological agents, and particularly relates to a high-efficiency trapping method of insect pathogenic nematodes, which adopts Galleria mellonella treated by immune barrier weakening and cocooning inhibition in sequence for trapping and collecting; and specifically comprises the following steps: S1, collecting fresh soil samples from the wild; S2, inoculating biocontrol bacteria into the soil samples, spraying pheromones and moisturizers, and mixing; S3, placing the Galleria mellonella treated in the above step into a trapping agent ball, and filling the soil samples treated in the step S2 into the trapping agent ball and compacting; S4, burying the trapping agent ball in the remaining soil samples, and after completion, placing the soil samples in a sample bag in a thermostat with sufficient oxygen for trapping, the application can improve the success rate of nematode infection, is beneficial to further propagation, collection and application of insect pathogenic nematode resources, and has the advantages of simple method and simple operation.

This invention discloses GmEXO70 Application of genes in enhancing soybean resistance to nematodes. This invention is the first to discover and validate a gene encoding the EXORDIUM protein through single-cell transcriptome analysis. GmEXO70 It is specifically and highly expressed in the syncytia of disease-resistant soybean varieties, and its expression is strongly induced by nematode infection. Overexpression was confirmed by constructing an overexpression vector and transforming soybean hairy roots with Agrobacterium rhizogenes. GmEXO70 It can significantly increase the expression level of this gene in roots. Further resistance evaluation showed that, compared with the empty vector control, overexpression significantly increased the expression level of this gene. GmEXO70 In the root system, the proportion of nematodes developing to the J3 / J4 stage was significantly reduced, with most nematodes arresting at the J2 stage. Furthermore, immunofluorescence in situ hybridization directly confirmed at the transcriptional level that... GmEXO70 The gene was specifically enriched in nematode-induced syncytia. This invention provides a new key gene for soybean nematode resistance breeding. GmEXO70 .

The invention relates to a microbial composition for preventing and treating root knot nematode disease of traditional Chinese medicinal materials and application thereof, and belongs to the technical field of biology. The composition is prepared from the following components: beauveria bassiana with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.21047, lecithin, xanthan gum, pyroligneous liquor, soybean peptide, potassium fulvate and medium trace elements. Through the synergistic effect of specific functional flora, the composition significantly promotes the growth of underground parts of traditional Chinese medicinal materials, improves the root biomass and root activity, enhances the nutrient absorption and utilization efficiency of plants, and especially improves the nitrogen utilization rate. Meanwhile, the preparation can effectively prevent and control the root-knot nematode disease, the number of root-knot nematode infection sources in soil and the severity of root diseases are remarkably reduced, and the high nematode natural decline rate is achieved. In addition, the preparation can activate the defensive system of plants, induce synthesis of disease-resistant substances and remarkably enhance the activity of multiple defensive enzymes. The soil micro-ecological environment is obviously improved, the diversity and richness of soil bacterial communities are improved, and the method is suitable for various crop planting systems.

The invention discloses a diagnosis target and a detection primer for wood infection of pine wood nematodes and application of the diagnosis target and the detection primer. According to the invention, through data analysis and comparison of miRNA changes with significant differences between a control group and an experimental group at different time points, six miRNAs which only exist in an inoculation group and do not exist in the control group are finally screened out; furthermore, a specific stem-loop DNA probe is designed by taking the six miRNAs as target genes to establish a pine wood nematode detection method, and according to a sensitivity detection result, the novel-miR574 with a nucleotide sequence as shown in SEQ ID No.13 has optimal detection sensitivity when being used as a diagnosis target for wood infection with pine wood nematode diseases; specificity test results show that whether a forest sample is infected with pine wood nematode or not can be specifically detected by using the novel-miR574 as the diagnosis target of the forest infected with the pine wood nematode disease; the invention has an application prospect in prevention and treatment of pine wood nematodes.