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Bacterium with induced resistance activity and cultural method and application thereof

A technology for inducing resistance and bacteria, applied in the field of biological strains, can solve problems such as soybean root diseases, and achieve the effect of promoting growth

Active Publication Date: 2015-12-02
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In response to the above problems, the present invention obtains a strain of bacteria through systematic screening, and uses the bacteria or metabolites of the bacteria to treat soybean seeds, which can induce soybeans to produce resistance to soybean cyst nematodes during seed germination and growth, Thereby solving the problem of soybean root diseases

Method used

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  • Bacterium with induced resistance activity and cultural method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Inoculate the strain of Enterobacter cloacae (E.cloacae) Sneb572 on the test tube culture medium, the medium formula is beef extract peptone medium, that is, the ingredients: beef extract 3g, yeast extract 1g, peptone 10g, sucrose 10g, agar 17 -20g, add distilled water to 1000mL. Incubate at 28°C for 3 days to obtain test tube species.

[0018] Then inoculate the test tube species into a 250mL triangular flask (150mL per bottle) liquid medium, and the medium formula is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3% -0.4%, 1%-2% sucrose, the rest is distilled water, pH is 6.8-7.0, fermentation temperature is 25-28°C, shaker speed is 150r min -1 , Fermentation time 2-3d. The resulting fermentation broth or finished product of the fermentation broth can be used for direct or indirect treatment of soybean seeds. Treated soybean seeds can reduce damage caused by soybean cyst nematode disease.

Embodiment 2

[0020] Field experiment on the control of soybean cyst nematode by the fermented product of E.cloacae Sneb572

[0021] Field effect tests were carried out in the experimental field of Shenyang Agricultural University in Shenyang City, Liaoning Province, the experimental field of Kangping City, Shenyang City, Liaoning Province, and the experimental field of Daqing Academy of Agricultural Sciences, Heilongjiang Province. The prepared fermented liquid stock solution is treated by seed coating with a volume ratio of 1:70, dried in the shade, and the treated soybean seeds are sown normally, and the soybean plants can produce soybean cyst nematode resistance at the seedling stage. resistance, and the induced resistance effect is relatively stable, and a good control effect has been achieved.

[0022] Step 1. Preparation of test preparations

[0023] The fermentation broth of Enterobacter cloacae (E.cloacae) Sneb572 was prepared according to the aforementioned liquid fermentation cu...

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Abstract

The invention discloses bacterium with an induced resistance activity and a cultural method and application thereof, wherein the bacterium is enterobacter cloacae and the preservation number of the enterobacter cloacae is CGMCC No. 10801. The bacterium with an induced resistance activity or the metabolite thereof can be used for preparation of a soybean seed treating agent and biological prevention and control of plant parasitic nematodes. The enterobacter cloacae (E.cloacae) Sneb572 or the metabolite thereof has an activity of inducing soybeans, can be used for soybean seed treatment, therefore not only is the growth of soybean plants and roots promoted, but also a great inhibiting effect on formation of heterodera glycines cysts is achieved, and the problem of prevention and control of heterodera glycines is solved.

Description

technical field [0001] The invention relates to the field of biological strains, in particular to a bacterium with the activity of inducing resistance and its cultivation method and application. Background technique [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual losses far exceed estimates, citing t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/01
Inventor 王媛媛段玉玺陈立杰朱晓峰闫继辰
Owner SHENYANG AGRI UNIV
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