Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci

A technology of leucococcus attenuatorium and Bemisia tabaci, which is applied in the field of leucococcus attenuatorium, can solve the problems of excessive pesticide residues in agricultural products, high toxicity, ecological environment pollution, etc., achieves great application potential, inhibits hatching, and has good parasitic effects. Effect

Inactive Publication Date: 2014-12-10
张艳军 +1
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevention and control of soybean cyst nematodes mainly relies on chemical pesticides, such as dibromochloropropane, dibromoethane, carbofuran, etc. Most of these chemical pesticides for nematode control are highly toxic pesticides, and long-term large-scale use has caused serious pesticide residues in agricultural products and pollution of the entire ecological environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci
  • Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci
  • Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Isolation of Lecanococcus attenuata bacterial strain of the present invention

[0020] With the aphids infected with Lecanococcus spp. collected from Cuba, after 3% NaClO surface disinfection, the dead worms are placed on the prepared medium, and the medium formula is PDA medium, that is, 200g of potatoes (peeled, cut into pieces and boiled 30min, gauze filter), glucose 20g, agar 20g, add distilled water to 1000mL, high temperature sterilization at 121°C for 30min. Place 3 dead worms in each petri dish, then place the petri dish in a constant temperature incubator at 27°C for cultivation, observe regularly, pick the growing hyphae onto PDA medium, and store them at 4°C after the mycelium is full. To be identified.

Embodiment 2

[0021] Embodiment 2: Colony character culture and morphological identification of the Lecanococcus attenuata bacterial strain of the present invention

[0022] The strain was inoculated on a PDA medium plate, cultured at 27°C, and the colony shape, color and colony growth of the strain were continuously observed until the colony covered 2 / 3 of the plate. After 10 days on the PDA medium, the colony of the strain is white, thin flocculent, and the back is colorless. Using the glass slide culture method, the PDA medium was made into small slices of 2cm×2cm, the activated mycelium of the fungus was picked on the medium, and placed in a 27°C constant temperature incubator for moisture cultivation, and continuously in the Olympus CX21BIM-SET6 microscope system Observe the formation of conidia and bottle stems of the fungus, measure and take pictures. The conidiophores of this strain are undeveloped and resemble vegetative hyphae, on which there are solitary, opposite or 3-4 whorls ...

Embodiment 3

[0023]Example 3: Molecular biology identification, ITS sequence alignment and phylogenetic analysis of the present invention's Lecanococcus attenuata strains. The CTAB method was used to extract the total DNA of the strains (Kim D H, Martyn RD, Magill C W. Restriction fragment length polymorphism groups and physical map of mitochondrial DNA from Fusarium oxysporum f. sp. niveum. Molecular Plant Pathology, 1992, 82: 346-353.). Use general primers ITS1-F and ITS4-R (White T J, Bruns T, Lee S. Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for physilogenetics. In: Innis M.eds. A PCR protocols: a guide to methods and applications. New York, USA: Academic Press, 1990, 315-322.), PCR amplification and sequencing of ITS sequences. PCR reaction system (20 μL): 1 μL of template DNA, 1 μL of PCR Master Mix (Thermo Fisher Scientific), and 1 μL of primers, supplemented with deionized water to 20 μL. Reaction conditions: 94°C for 4min; 94°C for 1min, 55°C for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses lecanicillium attenuatum and application of the lecanicillium attenuatum to controlling heterodera glycines. The lecanicillium attenuatum is lecanicillium attenuatum HNLA1306, which is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC No.9220. A fermentation liquor of the lecanicillium attenuatum can remarkably restrain incubation of eggs of heterodera glycines and has a strong killing effect on J2 larvas; the conidia of the lecanicillium attenuatum have a good parasitic effect on second-stage juveniles of bemisia tabaci; the lecanicillium attenuatum can be used for biological control of crop nematodes and bemisia tabaci.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a strain of Lecanococcus attenuata used for preventing and controlling crop nematodes and whiteflies. Background technique [0002] Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the important diseases restricting soybean production. Anderson T R, Arsyad D M, Tan Y, Ploper L D, Porta-Puglia A, Ram H H, Yorinori J T. Soybean disease loss estimates for the top ten soybean-producing countries in 1998. Canadian Journal of Plant Pathology, 2001, 23(2) : 115-121.) Since no germplasm resources with obvious disease resistance have been found so far, soybean cyst nematode disease is still a worldwide problem in agricultural production. The prevention and control of soybean cyst nematode mainly relies on chemical pesticides, such as dibromochloropropane, dibromoethane, carbofuran, etc. Most of these chemical pesticides for nematode control are high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00A01P7/04C12R1/645
Inventor 张艳军谢明尉文斌
Owner 张艳军
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap