Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof
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A technology for killing nematodes and plants, which is applied in the field of actinomycetes poisoning and killing plant-parasitic nematodes and the preparation thereof, and can solve the problems of strong sterilization, environmental pollution, and large waste of chemical nematicides.
Inactive Publication Date: 2008-07-23
SHENYANG AGRI UNIV
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At present, the control of plant parasitic nematodes is still dominated by chemical control, but in recent years, the application of chemical nematicides is wasteful, pollutes the environment, has poor selectivity, strong extinction, and damages soil biota. Zhang Keqin, He Shichuan, Zhou Wei, etc. 1991. The role
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Embodiment 1
[0017] Example 1: Cultivation of bacterial strain Snea253
[0018] A single colony of Snea253 was inoculated on the test tube agar medium, and the medium formula was GA medium, that is, KNO 3 1g; K 2 HPO 4 0.5g; MgSO 4 0.5g; NaCl 0.5g; FeSO 4 0.01g; 20g soluble starch; 17g agar; 1000mL water. Cultivate at 28°C for 5 days to obtain the test tube slant strain.
[0019] Then test tube slant strain is inoculated into 250mL triangular flask (50mL of every bottle) liquid medium, and medium formula is (percentage by weight): contain soybean cake powder 15%, soluble starch 10%, glucose 20%, ammonium sulfate 2.5% 、K 2 HPO 4 0.2%, CaCO 3 1%, NaCl 4%, the rest is water, and the pH is 7.2. At 25°C to 28°C, the shaker speed is 150r·min -1 ~200r·min -1 , fermentation 240h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.
Embodiment 2
[0020] Example 2: Nematicidal activity of strain Snea253:
[0021] On the basis of the stock solution of the fermentation broth, it was diluted successively to 1×, 5×, 10×, 15×, 20×, and the nematicidal activity tests were carried out on soybean cyst nematode second instar larvae, root-knot nematode incognita and soybean cyst nematode respectively. Cyst hatching inhibition assay.
[0022] 1. Preparation of preparations for testing
[0023] For the actinomycete strain Snea253 prepared by the aforementioned liquid fermentation culture method, the nematicidal activity test was carried out with different multiples of the fermentation broth.
[0024] 2. Preparation of Nematodes for Experiments
[0025] 2.1 Obtaining soybean cyst nematode eggs and second instar larvae
[0026]Soybean cyst nematode (Heterodera glycines): race 3 of soybean cyst nematode was collected from the experimental field of Northern Nematology Research Institute of Shenyang Agricultural University, and the c...
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Abstract
The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.
Description
Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to an actinomycete for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while ...
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