Microbe (SGM-1) and its preparing method
A technology of SGM-1 and microorganisms, which is applied in the field of microorganisms to achieve the effects of increasing the content of nutrients and organic matter, reducing pollution and promoting decomposition
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[0026] Collect rhizosphere soil samples, add sclerotia (50 grains / 100 g soil samples) and mix well, trap parasites at a constant temperature of 25°C; take them out after 4 weeks, rinse with tap water, 1% NaClO surface sterilization for 1 minute and sterile water After fully rinsing, put it on a filter paper sheet placed in a petri dish, and after 2 weeks of moisturizing culture at 25°C, check the hyperparasites on the surface of the sclerotia under a stereoscope, pick monospores and culture them on a PDA plate, and obtain them for purification and isolation.
[0027] The above-mentioned microorganisms showed strong parasitic ability to S. sclerotiorum sclerotia: after the amphibian monoaccremonium strain SGM-1 covered the petri dish, put the sclerotia that had been sterilized on the surface. After co-cultivation at 25°C for 2 weeks, the sclerotium was taken out, sterilized with 1% NaClO for 1 minute, rinsed with sterile water for 3 times, placed on the filter paper in the petri...
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