Microbe (SGM-1) and its preparing method

A technology of SGM-1 and microorganisms, which is applied in the field of microorganisms to achieve the effects of increasing the content of nutrients and organic matter, reducing pollution and promoting decomposition

Inactive Publication Date: 2005-06-01
中国农业科学院生物防治研究所
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this mycoparasitic phenomenon of nematode-predating hyphomycetes has been discovered for more than 20 years, only a small number of studies are limited to flat plates and sterilized soil

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Collect rhizosphere soil samples, add sclerotia (50 grains / 100 g soil samples) and mix well, trap parasites at a constant temperature of 25°C; take them out after 4 weeks, rinse with tap water, 1% NaClO surface sterilization for 1 minute and sterile water After fully rinsing, put it on a filter paper sheet placed in a petri dish, and after 2 weeks of moisturizing culture at 25°C, check the hyperparasites on the surface of the sclerotia under a stereoscope, pick monospores and culture them on a PDA plate, and obtain them for purification and isolation.

[0027] The above-mentioned microorganisms showed strong parasitic ability to S. sclerotiorum sclerotia: after the amphibian monoaccremonium strain SGM-1 covered the petri dish, put the sclerotia that had been sterilized on the surface. After co-cultivation at 25°C for 2 weeks, the sclerotium was taken out, sterilized with 1% NaClO for 1 minute, rinsed with sterile water for 3 times, placed on the filter paper in the petri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention is one kind of microbe and its preparation process. The microbe is amphibious Monacrosporium janus strain SGM-1 with preservation number of CGMCC No. 1036 used in biological prevention and control of plant Sclerotinia sclerotiorum and Heterodera glycines disease. The strain exhibits relatively strong parasitizing capacity on both Sclerotinia sclerotiorum and Heterodera glycines. Through inoculation with the antagonistic microbe, expanding culture and liquid fermentation, corresponding microbe slurry may be obtained; and the microbe slurry may be mixed with diatomite as carrier to prepare bacteria preparation for biological prevention and control of plant Sclerotinia sclerotiorum and Heterodera glycines disease.

Description

Technical field: [0001] The invention relates to the technical field of microorganisms, in particular to a microorganism and a preparation method thereof. Background technique: [0002] Plant parasitic nematodes are extremely harmful to agricultural and forestry production, and the global annual losses due to this amount to tens of billions of dollars. In my country, Heilongjiang Province alone causes losses of nearly 1 billion yuan due to soybean cyst nematodes (Heterodera glycines). Sclerotinia sclerotiorum is an important plant pathogenic fungus, which can infect a variety of crops, fruit trees, vegetables and commercial crops, especially causing serious damage to the yield and quality of rapeseed, soybean, sunflower, peanut, celery and cucumber. loss. There is no economically feasible way to control these two types of diseases at present, and biological control may be one of the important ways. [0003] Nematode-predating Hyphomycetes are important natural enemies of p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12N1/14
Inventor 李世东张拥华刘杏忠缪作清郭荣君
Owner 中国农业科学院生物防治研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap