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Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybean and application

A soybean cyst nematode, soybean technology, applied in the direction of application, bacteria, microorganism-based methods, etc., to achieve good results in solving soybean cyst nematode disease, cyst formation

Inactive Publication Date: 2018-05-08
项鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant studies have shown that Streptomyces sahahahubicin has growth-promoting and biocontrol effects, but the control of Streptomyces sahahahahmycin on soybean cyst nematode has not yet been reported

Method used

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  • Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybean and application
  • Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybean and application
  • Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybean and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the cultivation of sahahahmycin Streptomyces (Streptomyces zaomyceticus) Snea182

[0020] Firstly, the Streptomyces zaomyceticus (Streptomyces zaomyceticus) Snea182 strain was fermented and cultured, and then the metabolites after fermentation and cultured were tested for soybean-inducing activity to determine their inducing effect on soybean.

[0021] Streptomyces zaomyceticus (Streptomyces zaomyceticus) Snea182 thalline was inoculated on the culture medium, and the culture medium formula was Gao Shi No. 1 medium, namely composition: soluble starch 20g, NaCl 0.5g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.01g, 15-20g agar, pH 7.2-7.4, 1000mL distilled water, culture at 25°C for 2d-3d.

[0022] Inoculate the bacterial classification into 250mL Erlenmeyer flask (100mL per bottle) liquid culture medium again, the culture medium formula is: by weight percentage, soluble starch 20g, NaCl 0.5g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 ...

Embodiment 2

[0023] Embodiment 2: Fermentation of Streptomyces zaomyceticus Snea182

[0024] Basically the same as Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25° C., pH was 7.2, and fermented for 7 days.

Embodiment 3

[0025] Example 3: Verification of Soybean Systemic Resistance Induced by Streptomyces zaomyceticus Snea182

[0026] The induced systemic resistance of strain Snea182 to soybean cyst nematode was explored by split-root system. In the root splitting test, the challenge root system and the response root system were completely isolated from each other in space, and the Snea182 fermentation broth in the response root system had no direct contact with soybean cyst nematodes, and the control effect of the strain on nematodes was completely dependent on its induced resistance to plants. The assay method is reliable and has been widely used in the research of system-induced resistance of biocontrol microorganisms to soil-borne diseases.

[0027] 1. Preparation of preparations for testing

[0028] The fermented liquid of Streptomyces sahahahubicin Snea182 was prepared according to the aforementioned liquid fermentation culture method, and was set aside.

[0029] 2. Preparation of Nema...

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Abstract

The invention relates to a culture medthod for Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybeans after soybean seeds are treated with the strain and application of the strain. The actinomycetes strain Snea182 provided by the invention is already preserved in the China General Microbiological Culture Collection Center. After the strain is subjected tofermentation culture with a common liquid, a seed treatment agent for treating soybean seeds can be developed from metabolite of the strain, and the resistance of soybeans of the soybean seeds treatedwith the strain to heterodera glycines can be induced. After the soybean seeds are treated with the Streptomyces zaomyceticus Snea182 metabolite, remarkable resistance of soybeans to a first generation of heterodera glycines in a seedling period can be induced, a remarkable inhibition function on formation of heterodera glycines cytocyst is achieved, the strain is a potential biocontrol bacterium, and a novel idea for preventing and treating heterodera glycines diseases is developed.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a saahmycin streptomyces capable of inducing soybean resistance to soybean cyst nematodes and its application. Background technique: [0002] Soybean cyst nematode (Soybean Cyst Nematode, SCN), also known as soybean verticillium wilt and pitaya, is an important disease restricting soybean production. The disease has the characteristics of wide distribution, serious damage, wide host range, multiple transmission routes, and long survival time of dormant bodies (cysts). It is a soil-borne disease that is extremely difficult to control. The disease generally reduces the yield of soybean by 10% to 30%, and it can reach 70% to 80% in severe plots, which is more harmful than any single disease. Soybean cyst nematode disease causes an annual output loss of more than 9 million tons of soybean in the world, and an economic loss of 5.5 billion US dollars. In par...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 项鹏李宝华张武鹿文成李红鹏
Owner 项鹏
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