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A method for inducing maize female parent haploid

A maternal monoploid and ploidy technology, applied in biochemical equipment and methods, botany equipment and methods, plant products, etc., can solve time-consuming and labor-intensive problems, and achieve the effect of increasing induction rate and efficiency

Active Publication Date: 2019-05-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of breeding inbred lines is time-consuming and laborious, and it often takes more than 7 generations to breed a stable inbred line

Method used

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  • A method for inducing maize female parent haploid
  • A method for inducing maize female parent haploid
  • A method for inducing maize female parent haploid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the method for inducing maize maternal haploid

[0038] 1. Mapping of genes related to maize maternal haploid phenotype

[0039] By mapping the induction rate-related QTL in the maize maternal haploid Stock6-derived inducible line, the main QTL-qhir1 controlling haploid induction was obtained, and by functional annotation and candidate gene prediction of the genes in the mapping interval, A candidate gene ZmPLA was finally identified.

[0040] 2. Obtaining maize maternal haploid induction ability after knocking out the maize ZmPLA gene

[0041] 1. CRISPER / Cas9 system to knock out ZmPLA gene in maize

[0042] 1) Selection of sgRNA sequence

[0043] figure 1 Schematic diagram of gene structure and target sites.

[0044]The genome sequence of the maize ZmPLA gene is shown in Sequence Table 1. The sequence of the first exon of the maize ZmPLA gene is shown in Sequence Table 2 (Seq. 2 is at position 91-450 of Sequence 1).

[0045] The target site sequenc...

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Abstract

The invention discloses a method for inducing and producing corn female parent haploid. The method for inducing and producing the corn female parent haploid, provided by the invention, comprises the following steps: silencing or inhibiting expression of a ZmPLA gene in a target plant genome or knocking out a PLA (Polylactic Acid) gene to obtain a transgenic plant or an offspring thereof; obtainingthe corn female parent haploid in a selfing process or a process of taking the transgenic plant or the offspring thereof as a male parent to be hybridized with other materials. An experiment disclosed by the invention proves that mutation of ZmPLA can cause to production of the corn female parent haploid and an important foundation is laid for disclosing genetic and biological mechanisms for producing the corn female parent haploid. Meanwhile, a mutant single plant obtained by utilizing the experiment or the method has a haploid induction capability of a corn female parent and has great significance of selectively breeding a novel induction system, further improving the induction rate and improving the breeding efficiency of the corn haploid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for inducing maize female parent haploid. Background technique [0002] Corn is the largest crop in the world and has many uses such as food, feed and industrial processing. The increase in corn production is of great significance for supplying the current needs of food, feed and industrial processing. In the current situation where the arable land area is gradually decreasing, it is the key to cultivate high-yielding, multi-resistant and widely suitable maize hybrids. The breeding of maize hybrids depends on the selection of superior inbred lines. The traditional method of breeding inbred lines is time-consuming and laborious, and it usually takes more than 7 generations to breed a stable inbred line. In recent years, haploid breeding technology has the advantages of short breeding cycle, high efficiency, and easy combination with molecular marker-assisted br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N9/22A01H1/06A01H1/02A01H6/46
CPCA01H1/02A01H1/06A01H5/00C12N9/22C12N15/8213
Inventor 陈绍江刘晨旭钟裕陈琛
Owner CHINA AGRI UNIV
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