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Penicillium Chrysogenun inducing meloidogyne incognita chitwood resistance in tomatoes and use thereof

A technology for root-knot nematode incognita and tomato, applied in application, nematicides, fungi, etc., can solve problems such as environmental pollution, cost increase, human and animal hazards, achieve high toxicity and high residue, and prevent root-knot nematode incognita disease The occurrence and effects of the pandemic

Inactive Publication Date: 2012-09-05
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Foreign countries paid attention to the losses caused by nematodes in the 1920s. In the 1940s, high-efficiency nematicides were produced, but after continuous application of chemical nematicides, the phenomenon of rapid recovery of nematode density appeared (J.N. Sasse, 1985), and thus the amount of spraying must be increased, so not only the supply of pesticides is tight, but the cost is rising, and most importantly, the harm to humans and animals and the pollution to the environment are more serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Cultivation of Penicillium Chrysogenum Snef 1216

[0022] Firstly, the Penicillium Chrysogenum (Penicillium Chrysogenum) Snef 1216 strain was fermented, and then the metabolites after fermentation were tested for tomato-inducing activity to determine their inducing effect on tomato.

[0023] Inoculate the thalline of Penicillium Chrysogenum Snef 1216 on the test tube agar medium, the medium formula is PDA medium, namely ingredients: 7 potatoes (peeled) 200g, glucose (or sucrose) 10g, agar 17 -20g, add water to 1000mL. Incubate at 25°C for 7 days to obtain test tube seeds.

[0024] Inoculate the test tube into 250mL Erlenmeyer flask (100mL per bottle) liquid medium again, and the medium formula is: by weight percentage, NaNO 3 0.2%, K 2 HPO 4 0.1%, MgSO 4 .7H 2 O 0.05%, KCl 0.05%, FeSO 4 .7H 2 O 0.001%, sucrose 3%, add distilled water to 1000mL, pH is 7. Cultivate at 25°C, shaker speed at 150r min -1 1. After 7 days of fermentation, the fermented l...

Embodiment 2

[0025] Example 2: Fermentation of Penicillium Chrysogenum Snef 1216

[0026] Basically the same as Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25°C, pH was 7, and fermented for 7 days.

Embodiment 3

[0027] Example 3: Use of Fermented Product of Penicillium Chrysogenum (Penicillium Chrysogenum) Snef 1216

[0028] Use the fermented broth stock solution to process the coated tomato seeds at a ratio of 1:40. After drying, sow the treated tomato seeds and untreated tomato seeds in the soil inoculated with a large amount of root-knot nematode incognita, and carry out pot experiments. The treated seeds were found to be significantly less damaged than untreated seeds.

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PUM

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Abstract

The invention relates to a culture method and use of Penicillium Chrysogenun Snef 1216 inducing meloidogyne incognita chitwood resistance in tomatoes after treating tomato seeds and belongs to the technical field of microbial pesticides. The fungus strain Snef 1216 the invention relates to was collected in China General Microbiological Culture Collection Center on December 14, 2010. The collection number of the strain is CGMCCNO.4469. The strain is prepared by liquid fermentation culture. After being treated by the metabolite of the strain, the tomato seeds can generate meloidogyne incognita chitwood resistance during germination. The tomatoes treated by the metabolite of the fungus can effectively control the generation and spread of a disease caused by meloidogyne incognita chitwood, and have a bright application and development prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to Penicillium chrysogenum for inducing tomato resistance to root-knot nematode incognita and its application. Background technique: [0002] Plant parasitic nematodes (Meloidogyne.spp.) are a class of plant pathogens that parasitize crop roots and seriously endanger the world's agricultural production. They are one of the limiting factors for important crop production in many countries. In my country, this type of nematode has caused serious harm to many important crops such as tobacco, citrus, tomato, and cotton. It is estimated that the annual loss rate of major crops in the world due to plant parasitic nematodes is 12.3%, causing an annual loss of about 100 billion US dollars, but the actual loss is far more than estimated. Currently, cyst nematodes (Heterodera sp.) and root-knot nematodes (Meloidoyne sp.) are two types of nematodes that seriously dama...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/80
Inventor 段玉玺姜美英陈立杰王媛媛朱晓峰
Owner SHENYANG AGRI UNIV
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