A Stenotrophomonas bacterium that induces peanut resistance to root-knot nematode

A Stenotrophomonas, induced resistance technology, applied in the direction of bacteria, nematicides, microbial-based methods, etc., can solve the problems of reduced hatching rate of oocysts and increased mortality of second-instar larvae of M. northernis , to achieve a good inhibitory effect and the effect of promoting growth

Active Publication Date: 2020-01-17
SHENYANG AGRI UNIV
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  • Summary
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AI Technical Summary

Problems solved by technology

After treatment with this strain, the hatching rate of M. northernis oocysts was significantly reduced, and the mortality rate of the second instar larvae was increased.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Cultivation of Stenotrophomonas rhizophila Sneb 1777

[0019] Firstly, the Stenotrophomonas rhizophila Sneb 1777 strain was fermented and cultivated, and then the peanut-inducing activity test was carried out on the metabolites after fermentation to determine its inducing effect on peanut.

[0020] Stenotrophomonas rhizophila (Stenotrophomonas rhizophila) Sneb 1777 strain was inoculated on the test tube medium, and the medium formula was YMA medium, namely ingredients: mannitol: 10g, yeast powder: 0.80g, K 2 HPO 4 : 0.25g, KH 2 PO 4 : 0.25g, MgSO 4 .7H 2 O: 0.20g, NaCl: 0.10g, agar: 15.0g, distilled water: 1000mL. Incubate at 28°C for 3 days to obtain test tube species.

[0021] Then inoculate the test tube into a 250mL triangular flask (150mL per bottle) liquid medium, the medium formula is: tryptone: 5g, yeast powder: 3g, CaCl 2 .2H 2 O: 0.7g, distilled water: 1000mL, adjust the pH to 6.8-7.0. The fermentation temperature is 25-28°C, the rotation s...

Embodiment 2

[0022] Embodiment 2: Stenotrophomonas rhizophila (Stenotrophomonas rhizophila) Sneb 1777 fermented product prevents and controls root-knot nematode greenhouse pot planting effect test

[0023] In order to verify the stability of its induced resistance, the research on the control effect of 5 potted repetitions was hereby carried out.

[0024] 1. Test preparation

[0025] The fermentation broth of Stenotrophomonas rhizophila Sneb 1777 was prepared according to the aforementioned liquid fermentation culture method, and it was ready for use.

[0026] 2. Peanut varieties used in the test

[0027] Baisha 1016, provided by Peanut Research Institute of Liaoning Academy of Agricultural Sciences.

[0028] 3. Seed treatment method

[0029] Disinfect the surface of peanut seeds with 0.1% mercury chloride solution for 2 minutes, and then rinse them with sterile water for 5-10 times. The prepared fermented liquid is subjected to seed coating treatment with a liquid seed volume ratio of...

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Abstract

The invention relates to a method for cultivating a bacterium capable of inducing plant anti-parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The bacterial strain involved in the present invention is Stenotrophomonas rhizophila Sneb 1777, depository unit: General Microbiology Center of China Microbiological Culture Collection Management Committee; address: Zhongguancun, Beijing, China; preservation date: July 6, 2015; The number of the deposit registry: CGMCCNo.11046. The taxonomic status of Sneb 1777 is Proteobacteria, Proteobacteria C, Xanthomonas, Xanthomonas, Stenotrophomonas, and Stenotrophomonas rhizogenes. Prepared by liquid fermentation culture, the fermentation metabolites of peanut seeds or peanut plants can induce peanut roots to develop resistance to peanut root-knot nematode disease, and the strain can be used to produce biological seed coating agents or attractants for preventing and treating peanut root-knot nematode disease. Antibiotics, with good prospects for application and development.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a bacterium Stenotrophomonas rhizophilus for inducing peanut resistance to root-knot nematode disease. Background technique: [0002] Root-knot nematodes can cause harm to more than 3,000 species of host plants in 114 families. Therefore, it is urgent to find efficient and environmentally friendly methods to prevent and control root-knot nematode diseases. Peanut is the main oil crop and economic crop in my country, with an annual planting area of ​​about 50 million mu. Peanut root-knot nematode is one of the devastating diseases in peanut production. It occurs in most of the main peanut production areas in my country. Since its discovery, it has been an important limiting factor restricting the continuous increase of peanut production. The affected peanuts generally reduce production by 20%- 30%, severe ones reach 70%-80%, or even extinction (Dong Weibo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P5/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 段玉玺回虹燕陈立杰朱晓峰王媛媛刘晓宇
Owner SHENYANG AGRI UNIV
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