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Cold-resistance-inducing Pseudomonas aeruginosa strain and application thereof

A soybean, cold resistance technology, applied in the field of microbial pesticides, can solve problems such as crop quality and soil impact

Inactive Publication Date: 2012-09-05
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current method to improve the cold resistance of soybeans is to use some chemical substances or anti-cold agents, etc., which may have a certain impact on crop quality and soil

Method used

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  • Cold-resistance-inducing Pseudomonas aeruginosa strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the acquisition of Pseudomonas aeruginosa (Pseudomonas aeruginosa) Sneb179

[0021] Take 1g of soybean field soil, add 100ml of sterile water, take 200μl, add 20ml of sterilized NA liquid medium cooled to below 35°C, cultivate at 25°C and wait for colonies to grow. Pseudomonas aeruginosa (Pseudomonas aeruginosa) colony is preserved separately, carries out multiplication and physiological and biochemical assay, and the culture trait conforms to the characteristic of Pseudomonas aeruginosa (Pseudomonas aeruginosa) and preserves for future use.

Embodiment 2

[0022] Embodiment 2: the cultivation of Pseudomonas aeruginosa (Pseudomonas aeruginosa) Sneb179

[0023] Firstly, the Pseudomonas aeruginosa (Pseudomonas aeruginosa) Sneb179 strain was fermented and cultured, and then the metabolites after fermentation and cultured were tested for soybean-inducing activity to determine their inducing effect on soybean.

[0024] Pseudomonas aeruginosa (Pseudomonas aeruginosa) Sneb179 thalline was inoculated on test tube agar medium, and the medium formula was NA medium, namely ingredients: beef extract 3g, peptone 10g, yeast extract 1g, sucrose 10g, agar 20g, add water to 1000mL. Incubate at 25°C for 10 days to obtain test tube seeds.

[0025] Test tube seed is inoculated in the liquid culture medium of 250mL triangular flask (every bottle 100mL) again, and medium formula is: by weight percentage, containing beef extract 0.2%, peptone 1%, yeast extract 0.5%, sucrose 1%, The remainder was distilled water with a pH of 8. Cultivate at 25°C, sha...

Embodiment 3

[0026] Example 3: Fermentation of Pseudomonas aeruginosa Sneb179

[0027] Basically the same as Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25° C., pH 8, and fermented for 48 hours.

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Abstract

The invention relates to a culture method and application of a Pseudomonas aeruginosa Sneb179 strain, which can induce soybeans to resist low temperature in early spring after being used for treating soybean seeds, belonging to the technical field of microbial pesticides. The bacterial strain Sneb179 provided by the invention was preserved in the Center of Common Microorganisms in China Committeefor Culture Collection of Microorganisms on November 2nd, 2010, and the preservation number is CGMCC No.4295. The Pseudomonas aeruginosa strain is fermented and cultured by using a liquid; and after the metabolite of the Pseudomonas aeruginosa strain is used for treating soybean seeds, the soybeans can resist low temperature in early spring. The soybeans treated by the metabolite of the Pseudomonas aeruginosa can be sowed in early spring, and have certain cold resistance. The invention solves the problems of less effective accumulated temperature and short seasons for soybean growth in high-latitude areas all the year around, and has wide application development prospects.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, in particular to Pseudomonas aeruginosa for inducing soybean cold resistance and its application. Background technique: [0002] Northeast my country is the main soybean producing area in China, and low temperature is the main factor reducing soybean production. Soybean is prone to chilling injury at all stages of the entire growth period, thereby reducing yield and quality, especially the early germination stage is sensitive to low temperature. When sowing in early spring, due to the low temperature, it is easy to prolong the emergence time and cause deformed seedlings, or the seeds will lose their germination ability due to mildew and deterioration, resulting in a reduction in soybean yield. Therefore, strengthening the relevant research on the low temperature tolerance of soybean emergence has positive significance for soybean production. The cold resistance of plants can be imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01C1/08C12R1/385
Inventor 段玉玺黄姗姗陈立杰朱晓峰王媛媛
Owner SHENYANG AGRI UNIV
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