95 results about "Penicillium chrysogenum" patented technology

The invention relates to promoters of the genes glutamate dehydrogenase, beta-acetylhexosaminidase and gamma-actin and their use in systems of expression, secretion and anti-sense of filamentary fungi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate dehydrogenase NADP depending (EC.1.4.1.4) of Penicillium chrysogenum, (II) gamma-N-actylhexosaminidase (EC.3.2.1.52) of Penicillium chrysogenum and (III) gamma-actin of Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. These promoters can also be used for blocking the genic expression through anti-sense construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of antibiotics and / or proteins inherent to the same.

The invention discloses a biological leaching restoring method for heavy metal polluted soil. After Penicillium chrysogenum F1 spore liquid is cultured in an improved Czapek liquid medium, leaching fungus liquid is obtained, and then the heavy metal polluted soil is leached, dried and crushed. By restoring the heavy metal (Pb, Zn, Cd and Cu) compound polluted soil according to the biological leaching restoring method provided by the invention, the removing rate of Pb in the soil is 14%, the removing rate of Cd is 63%, the removing rate of Zn is 54% and the removing rate of Cu is 56%. The biological leaching restoring method for the heavy metal polluted soil provided by the invention has the advantages of high speed in taking effect, high efficiency in removing heavy metal, absence of secondary pollution, and the like.

The invention belongs to the technical field of biological materials and application thereof, and provides a block koji strengthening method capable of reducing the dosage of block koji and improving the quality of vinegar, aiming at solving the problems that vinegar which is brewed by pure block koji has a good flavor, but the saccharifying capability and the alcoholization capability are low and the vinegar yield is low. The block koji strengthening method comprises the following steps: separating fungus strains with high yields of cellulase and amylase; culturing single strains and commonly strengthening the block koji through penicillium chrysogenum and saccharomycopsis fibuligera; carrying out acetic acid fermentation. An exogenous catalyst does not need to be added, the dosage of the block koji is reduced and the utilization rate of raw materials is improved. The block koji strengthening method is simple and convenient to operate; unique flavor and quality of Shanxi traditional mature vinegar are kept, and the product yield is also improved; the quality of the vinegar is improved, the cost is reduced and the benefits are increased. When the block koji strengthening method is popularized and applied, the revolution of a Shanxi traditional mature vinegar process is brought, so that the development of Shanxi mature vinegar industries is promoted and corresponding economic and social results are generated; the block koji strengthening method has a wide application and popularization prospect.

The embodiment of the invention discloses a bacillus subtilis strain and application thereof. The bacillus subtilis strain is collected in China General Microbiological Culture Collection Center, hasa collection number of CGMCC NO. 16569, and has a collection date of October 10, 2018. The embodiment of the invention further provides high-density bacillus subtilis fermentation liquor containing bacillus subtilis strain metabolite. The bacillus subtilis strain provided by the embodiment of the invention is capable of effectively inhibiting aspergillus carbonarius, aspergillus niger and penicillium chrysogenum, while the aspergillus carbonarius, aspergillus niger and penicillium chrysogenum are mold that easily grow on grapes. Therefore, the high-density bacillus subtilis fermentation liquorcontaining the bacillus subtilis strain metabolite is capable of effectively prolonging the grape storage period, has the characteristics of being high-efficiency, safe, environment-friendly and thelike, and has wide application prospects.

The invention discloses a natural food preservative. The natural food preservative is compounded by effective antibiotic parts of Forsythia suspensa leaves and traditional Chinese medicine Scutellaria baicalensis, wherein the effective part of the Forsythia suspense leaves is 30%-70% (mass percent), and the others are the effective parts of Scutellaria baicalensis. The natural food preservative has stronger growth inhibition effect on the bacteria, such as staphylococcus aureus, escherichia coli, bacillus subtilis, saccharomyces cerevisiae, Aspergillus flavus and Penicillium chrysogenum, which are common and are likely to cause food spoilage. The Forsythia suspensa leaves are mainly used for making Forsythia suspensa leave (Forsythia suspensa) tea in civil society, and have the effects of clearing heat and removing toxicity, cooling, and so on. The Scutellaria baicalensis is a common traditional Chinese medicine, and has the effects of clearing heat and drying dampness, and removing toxicity. Thus, the natural food preservative compounded by effective antibiotic parts of the Forsythia suspensa leaves and the Scutellaria baicalensis is nontoxic, has no side effect, has better bacterial growth inhibition effect, and can be used for prolonging the food spoilage and preservation time for various foods.

The invention belongs to the field of preparing biological coalbed methane by microorganisms, and discloses a compound microorganism bacterium agent of the biological coalbed methane prepared by coal bed organic impurities and an application of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises fermentation broths of four bacteria namely Phanerochaetechrysosporium, Penicilliumchrysogenum, Pseudomonasecpacia and Clostridiumacetobutylium. The compound microorganism bacterium agent disclosed by the invention has strong digestive ability to various celluloses, humus and various carbon-containing macromolecules in the coal bed. A coal added with the compound microorganism bacterium agent can accelerate the enrichment of methanogenus, shorten anaerobic digestion time and improve the output of the biological coalbed methane; and in addition, the synergy effects of the four microorganism strains are superior to the effect of any one, two or three microorganisms.

The invention relates to an extraction method of a mycelial fungus genome. Enzymolysis is performed on Penicillium chrysogenum, Aspergillus niger, Trichoderma reesei, penicillium citrinum and other mycelial fungus hyphae by using a helicase-cellulase mixed enzyme solution, and the enzymolysis treatment is effectively combined with ultrasonic treatment and high-temperature heating treatment, thereby obtaining the mycelial-fungus-genome-containing supernate for PCR (polymerase chain reaction) identification. The supernate of the mycelial fungus hypha enzymolysis solution obtained by the extraction method is directly used for PCR reaction to obtain the mycelial fungus 18S rDNA gene strip without purification operation. The operational process is simple, safe and stable, avoids the use of the toxic extraction reagent, and reduces the injury to the experimental personnel.

The invention relates to a method for preparing shenqu by purebred fermentation. The method is characterized by using Penicillium chrysogenum as a single fermentation bacterial strain, and employing six materials such as flour (and or wheat bran), red bean, bitter apricot kernel, sweet wormwood, water pepper, and Xanthium sibiricum to prepare a culture medium; the six materials are fermented under proper conditions to prepare medicine shenqu.

This invention describes a penicillium chrysogenum strain and its application id the production of penicillin. The strain described in this invention is penicillium chrysogenum CGMCC No.1248, and has simple cultivation conditions as well as a high yield. This invention also describes the process for producing penicillin from the said strain.

The present invention discloses an airlift type loop reactor and a method for penicillin fermentation by using the airlift type loop reactor. The lower part of the tower body of the airlift type loop reactor is the reaction section, and the upper part is the expanded gas-liquid separation section, wherein a height ratio of the gas-liquid separation section to the reaction section is 0.15-0.25, a diameter ratio is 1.25-1.42, a ratio of the total height of the tower body to the inner diameter of the reaction section is 4.5-8, a ratio of the inner diameter of a draft tube to the inner diameter of the tower body is 0.5-0.78, and a height ratio of the draft tube to the reaction section is 0.9-0.65. In the airlift type loop reactor, air is introduced, and a strain producing penicillium chrysogenum is adopted to produce penicillin, wherein the fermentation temperature is 20-30 DEG C, the pH value is 6.0-7.0, the fermentation pressure is 0.08-0.1 MPa, the ventilation ratio is controlled to 0.8-3 vvm, and the fermentation time is 160-170 hours.

The invention relates to a culture method and use of Penicillium Chrysogenun Snef 1216 inducing meloidogyne incognita chitwood resistance in tomatoes after treating tomato seeds and belongs to the technical field of microbial pesticides. The fungus strain Snef 1216 the invention relates to was collected in China General Microbiological Culture Collection Center on December 14, 2010. The collection number of the strain is CGMCCNO.4469. The strain is prepared by liquid fermentation culture. After being treated by the metabolite of the strain, the tomato seeds can generate meloidogyne incognita chitwood resistance during germination. The tomatoes treated by the metabolite of the fungus can effectively control the generation and spread of a disease caused by meloidogyne incognita chitwood, and have a bright application and development prospect.

The invention discloses high-cadmium-adsorption filamentous fungi penicillium chrysogenum J-5 as well as a preparation method and an application thereof. The preparation method comprises the following steps of: firstly weighing and putting 10g of cadmium polluted soil into 90mL of sterilized normal saline, vibrating on a constant-temperature shaking table, standing, taking supernatant liquid and diluting; secondly coating different dilution factors of diluent on a Martin rose-bengal flat plate containing 10mM of cadmium, and inversely placing the flat plate in a constant-temperature incubator for cultivation; thirdly, carrying out streaking and purification on microorganisms which grow on the flat plate, and then coating the organisms on the Martin rose-bengal flat plate containing cadmium again for cultivation; fourthly, after isolation and screening, storing a bacterial strain having stable resistance capacity on cadmium on a PDA (Potato Dextrose Agar) inclined plane. The obtained filamentous fungi penicillium chrysogenum J-5 has high tolerance and high adsorption property on multiple metals; the raw materials are easy to obtain and low in cost. The bacterial strain has relatively high fixation capacity on heavy metals in environments, can be used of reducing the biological toxic property of the heavy metals and is suitable for the in-situ remediation of large-area polluted soil.

The invention relates to a method for preparing nano silver through pyrenochaeta penicillium and belongs to the field of nano material preparation. According to the method, a pyrenochaeta penicillium chrysogenum soak solution mainly serves as a reaction solution to prepare the nano silver, operation is easy, no other reducing agent or additive needs to be added, the whole process is environmentally friendly, safe and non-toxic, reaction conditions are safe and controllable, the nano silver prepared through the method is spherical or approximately spherical, and the particle size is 5-60 nm. Due to the fact that reductase and other bioactivators in the pyrenochaeta penicillium chrysogenum soak solution participate in the process that silver nitrate is reduced into the nano silver and also wrap the surfaces of silver nano particles after the silver nano particles are formed, protein coats are formed, compared with nano silver and silver nitrate which are synthesized through a chemical method, the nano silver has unique biological characteristics and good biocompatibility, the nano silver can enter microbial cells more easily and disturb normal metabolism of microorganisms so that the microorganisms can be dead, thus, better bacteriostatic activity is achieved, application of the nano silver in the field of biomedicine is more advantageous, and the application prospects are broader.

The invention relates to a huperzine A high yield strain TCM-01 of which classified nomenclature is Endophyte sp.TCM-01, conservation address is the China center for type culture collection, conservation date is April 16, 2010 and CCTCC NO is M2010086. The high yield strain uses Penicillium chrysogenum Thom as parent strain and is prepared through microwave mutagenesis-screening. The invention further optimizes the condition of fermentation culture of the strain; and under the condition of culture, the yield of huperzine A is up to 4.761mu g / ml. Under the same condition of fermentation, the yield of the product is greatly increased, and the cost can be largely saved.

The invention discloses a method for improving the salt resistance of turf by adopting a reinforced garbage compost microbial agent. The reinforced salt resistant microbial agent comprises the following components: reinforced bacillus subtilis, reinforced lysine bacillus and reinforced penicillium chrysogenum, with the weight ratio of 1: 1: 1. The method disclosed by the invention adopts the following steps: gradually increasing the concentration of NaCl to reinforce microorganisms in urban garbage compost, and finally performing the screening so as to obtain the prepared reinforced salt resistant microbial agent. The obtained reinforced salt resistant microorganisms of the reinforced bacillus subtilis, the reinforced lysine bacillus and the reinforced penicillium chrysogenum are compounded into composite microbial agents in different concentrations, the composite microbial agents are inoculated to a planting system of the turf, so that the optimal concentration of the composite reinforced salt resistant microbial agent is screened out, the salt resistance of the turf is improved, and the technical support is provided for planting the carpet turf on the substrate of saline-alkali soil.

The invention belongs to the field of restoration of heavy metal pollution in soil, and discloses a Penicillium chrysogenum strain. The strain is collected at China Center for Type Culture Collection, and the collection number is CCTCC NO:M2013604. The invention also discloses application of the Penicillium chrysogenum CH03 strain in restoring heavy metal pollution in soil and a method for restoring heavy metal pollution in soil. The method can enhance the yield of lettuce and wheat growing in a heavy-metal-polluted field and lower the contents of copper and cadmium in plant tissues, and thus, is a method for restoring heavy-metal-polluted soil in situ by using a microbe-organic substance combination. The method is simple to operate, has the advantages of low cost, no need of stopping farming and favorable restoration effect, and thus, is an environment-friendly method for restoring heavy-metal-polluted soil.

Methods for an improved culturing of the fungus Penicillium chrysogenum, in particular strain KIP 3201, for an optimised production of the anti-tumour natural compound sorbicillactone A (2), and derivatives thereof in large amounts as well as optimised methods for the recovery and purification thereof from the fungal biomass and from the culture medium are described. Furthermore, the biological activity of sorbicillactone A (2), and studies regarding the genotoxicity of the compound are described.

The invention discloses a complex bacterial preparation for degrading non-wood-fiber lignin and a preparation method thereof. The preparation method is characterized by comprising the steps of inoculating and culturing aspergillus fumigatus, penicillium chrysogenum and common penicillium, wherein the inoculating ratio of the aspergillus fumigatus, the penicillium chrysogenum and the common penicillium is 0.5-2:3-5:15-17; carrying out mixed culture for degrading reed lignin. According to the invention, through the mixed culture of strains, complementary and synergistic effects exist between strains, so that the conversion rate is improved in effective time, and the efficiency of reed lignin degradation can be greatly improved.

The invention discloses a method for improving protective enzyme activities of salt-stressed turfgrass by adopting an enhanced salt-tolerant microbial agent. The enhanced salt-tolerant microbial agent comprises enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum in a weight part ratio of 1 to 1 to 1, wherein enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum are salt-tolerant mixed microbial flora obtained by carrying out domestication step by step with a method for increasing the salt concentration step by step. The invention further discloses an application of the enhanced salt-tolerant microbial agent in improvement of the protective enzyme activities of salt-stressed turfgrass festuca arundinacea L. Experimental results show that the compound enhanced microbial agent can conduce to effectively alleviating the harms of saline-alkali soil to the turfgrass; screening and culturing corresponding enhanced salt-tolerant compost microbial strains and carrying out artificial inoculation is a new way for improving the salt tolerance of the turfgrass.

The invention discloses a compound microorganism combination microbial agent, a preparation method and application thereof, a comprehensive treatment method for a black-odor river. The compound microorganism combination microbial agent comprises the three kinds of microbial agent combinations of a microbial agent A combination, a microbial agent C combination and a microbial agent gamma combination, and the microbial agent A combination comprises the microbial strains of bacillus coagulans, lactic acid bacteria, saccharomycetes, aspergillus sojae and thiobacillus denitrificans; the microbial agent C combination comprises the microbial strains of bacillus coagulans, rhizopus oryzae, saccharomycetes, aspergillus sojae and penicillium chrysogenum; and the microbial agent gamma combination comprises the microbial strains of lactic acid bacteria, bacillus subtilis, saccharomycetes, bacillus licheniformis and bacillus coagulans. The compound microorganism combination microbial agent can be used for comprehensive treatment of the black-odor river, the treatment period is short, the situation that nitrogen and phosphorus of a black and odorous water body exceed a standard can be solved, and odorous gas hydrogen sulfide in the water body can be eliminated.

The present invention provides a method for producing a novel β-lactam antibiotic from a protoplast fusion strain. The method is to fermentatively culture the protoplast fusion strain of Penicillium chrysogenum and Cephalosporium acremonium. The ferment filtrate is isolated, lyophilized, and extracted by acetone or acetone / methanol. The extract is concentrated by decompression, and then analyzed by preparation type HPLC to isolate the active antibiotic compound.

The present invention discloses Penicillium chrysogenum producing fungi having plant poison activity, a preparation method and applications thereof. The fungi is Penicillium Chrysogenum and has characteristics of radial wrinkles, white edge mycelium, velvet-like texture, a large number of conidium structure, blue-green color, yellowish exudate and soluble pigment, and slight tawny colony back surface. In addition, the metabolites of the fungi provide significant plant poison activity and nematode contact toxicity activity for other plants.

The invention discloses a recombinant penicillium chrysogenum gene engineering strain and a construction method and an application thereof. The sequence of the Pc-somA gene in the penicillium chrysogenump gene engineering strain is replaced by the Bleo resistance gene. The penicillium chrysogenum is ATCC48271; the construction method is as follows: (1) extracting genomic DNA of penicillium chrysogenum; (2) constructing a gene knockout fragment; (3) preparing protoplasts of penicillium chrysogenum; and(4) introducing the gene knockout fragment into the prepared protoplast for homologous recombination to obtain recombinant penicillium chrysogenum gene engineering strain. Beneficial effects are as follows: the gene engineering strain provided by the invention can increase the biofilm amount OD570 of penicillium chrysogenum by nearly 2 times in the process of immobilized fermentation for penicillin production, the hypha can be better adsorbed and combined with a plastic medium, the titer of penicillin is increased to 1963.25 U / mL, the whole fermentation period is shortened by 24 hours, and the production cost is reduced.

The present invention relates a novel compound, chrysogenazine containing both indole and diketopiperazine ring systems, isolated from the chloroform fraction of the fermentation broth of Penicillium chrysogenum and the gross structure of the compound was elucidated by a detailed analysis of spectroscopic data (IR, NMR, MS), in addition, this invention also assesses the biological activity of the compound which reveals its antibacterial activity against the human pathogen, Vibrio cholerae, demonstrated by the disc diffusion assay.

The invention discloses a broad-bean straw compost composition and a piling method thereof. The composition comprises the following ingredients in parts by weight: 75-95 parts of sundried broad bean straw, 135-155 parts of sundried faeces, 1 part of a fermentation bacteria liquid, and 52-72 parts of water, and the fermentation bacteria liquid is formed by mixing penicillium chrysogenum, bacillus subtilis and trichoderma viride according to the weight ratio of 2:2.5-3.5:2.5-3.5. According to the technical scheme, the fermentation inoculum is optimized, the optimized fermentation bacteria liquid is strong in pertinency, thorough decomposition of broad bean straw nutrition is realized, thorough sterilization is realized, and thus the compost turning-over frequency is reduced, and the step of subsequently adding an inoculum is avoided.

The invention discloses preservative added into foods. The preservative comprises, by volume, 23-28 ml of sodium benzoate, 2-6 ml of butyl-p-hydroxybenzoate, 30-50 ml of propylene glycol, 2-8 ml of fatty acid monoglyceride, 4-10 ml of fructus forsythiae leaf extractives, 0.2-0.8 ml of dehydrated sorbitan esters of fatty acid and 50-60 ml of water. By means of the mode, the preservative added into the foods is low in cost, has the efficient and broad-spectrum antibacterial effect, has the inhibition effect on Escherichia coli, Aspergillus flavus, penicillium chrysogenum, staphylococcus aureus, and can be used for preservation of the foods such as aquatic products, livestock meat, soft drink and noodles.

The invention discloses penicillium chrysogenum with high salt and alkali resistance as well as a separation method and an application thereof. The method comprises the following steps: coating a cornstraw powder culture medium solid flat plate containing 250.0 g / L NaCl with different concentrations with a straw powder suspension subjected to gradient dilution, treating and repeating for 3 times,culturing for 4-15 days, picking well-grown single colonies, inoculating the single colonies onto a screening culture medium CMC-Na solid culture medium, and generating a transparent ring-shaped strain to obtain penicillium chrysogenum with a preservation name of penicillium chrysogenum W2 and a preservation number of CGMCC NO. 17972. The penicillium chrysogenum with high saline-alkali resistancehas high impermeability, is beneficial to improving the degradation capacity of straws in a high saline-alkali environment, can also be directly used for decomposing straw fermentation and other biological substances in fields, and has good effects on improvement of secondary salinization or continuous cropping obstacle soil and environmental pollution treatment.

The invention discloses a microbial method for quickly treating a lead and zinc polluted water. The microbial method comprises the following three steps: purification culture of microorganisms, complexation of heavy metal ions and catalysis on lead and zinc ion adsorption, and is characterized in that the purification culture of the microorganisms at least comprises the purification culture of penicillium chrysogenum and bacillus; the complexation of the heavy metal ions specifically comprises complexation of nitrogen, oxygen, sulfur, phosphorus and other atoms on phosphoryl groups or carboxy groups on the surface of the penicillium chrysogenum or the bacillus with lead and zinc ions; a catalyst used during the catalysis on the lead and zinc ion adsorption is a synthesis promoting enzyme; the purification culture of the penicillium chrysogenum and the bacillus at least comprises the following steps: 1, strain purification; 2, culture of a target strain; 3, microorganism preservation and preparation of a microbial preparation. The microbial method for quickly treating the lead and zinc polluted water is a heavy metal treatment method capable of treating lead and zinc pollution of the water, achieving in-situ repair, avoiding secondary pollution to the environment and having high degradation efficiency.

The invention provides a lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity. The strain is preserved in China General Microbiological Culture Collection Center on July 6, 2020, and the preservation number is CGMCC No.20198. The lactobacillus plantarum PC28152 and the fermentation liquor thereof have the effect of inhibiting fungal activity in a broad spectrum, and particularly have an obvious inhibiting effect on aspergillus flavus, penicillium chrysogenum, penicillium expansum, fusarium moniliforme and cladosporium cladosporioides. When the strain is applied to food preservation and preservation, infection of spoilage fungi to food can be effectively inhibited, and the shelf life of the food is remarkably prolonged.

This invention involves the new encoding hydroxylase gene (pahB) from Penicillium chrysogenum, the gene encoding the peptide and containing the gene expression vector. It also demonstrated that the phenyl acetic acid can significantly induce the expression of pahB. The invention also provides a direction and target for increasing the production of penicillin from genitic modified Penicillium Chrysogenum, and is of great importance to the production of penicillin.