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Penicillium chrysogenum producing fungi having plant poison activity, preparation method and applications thereof

A technology for producing Penicillium chrysogenum and Penicillium genus, applied in the field of microbial pesticides, can solve problems such as serious environmental pollution, increase in the population of drug-resistant weeds, crop phytotoxicity, etc., achieve no pollution to the environment, simple production process, and low production cost Effect

Inactive Publication Date: 2015-06-10
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the continuous application of chemical herbicides has also caused phytotoxicity of crops, an increase in the population of drug-resistant weeds, and environmental pollution has become increasingly serious

Method used

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  • Penicillium chrysogenum producing fungi having plant poison activity, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Isolation of Plant Rhizosphere Soil Fungi by Serial Gradient Dilution

[0026] 1. Isolation of endophytic fungi from Penicillium chrysogenum

[0027] Strain F18 was isolated from red clover seeds purchased from Lanzhou seed market. The method is as follows: immerse 1 g of seeds in 1 volume of alcohol, shake fully for 5 min, filter out the alcohol, rinse with sterile water and filter out the solution, add 800 μL of water, grind the seeds to powder, and then take 10 times the volume as the unit Perform serial dilutions until a minimum concentration of 10 is obtained -8 serial dilutions, from a concentration gradient of 10 -4 -10 -8 Take 0.1 ml of the diluent, spread evenly on the Martin's plate medium, and incubate at 28±2°C in the dark. Purify on new Martin's medium, without contamination, then transfer to Martin's slant medium and store at 4°C for a short time.

[0028] The specific composition of Martin's medium is consistent with that in the summary of...

Embodiment 2

[0030] Embodiment 2. The cultivation of bacteria and the preparation of metabolites

[0031] 1. Cultivation of primary strains: Transfer and activate the strain F18 stored on the slant medium to Martin's plate medium, and cultivate in the dark at 28-30°C. After the colonies visible to the naked eye grow, they will be used as primary strains .

[0032] 2. Cultivation of secondary strains: inoculate the activated primary strains on Martin's liquid medium, place them on a shaking table with a temperature of 28-30°C and a rotation speed of 180 rpm, and cultivate them in the dark, observe and record them regularly, and wait until After the culture medium becomes turbid (about 7 days), stop the shaking culture and transfer to a 4°C refrigerator for short-term storage.

[0033] 3. Preparation of metabolites: After sterilizing the fungal fermentation liquid, filter it, then extract it with ethyl acetate, concentrate the ethyl acetate extract, and obtain the secondary metabolites of ...

Embodiment 3

[0034] Example 3. Phytotoxic activity of secondary metabolites of Penicillium chrysogenum F18

[0035] 1. Cultivation of test plants

[0036] In this embodiment, model plants Arabidopsis thaliana, weeds Bluegrass and Tallgrass are used as experimental materials. Arabidopsis was cultured on MS medium for 7 days before the phytotoxicity test; bluegrass and tall thatch were cultured on sterile filter paper for 5-7 days before the phytotoxicity test.

[0037] 2. Phytotoxic activity of metabolites at different concentrations

[0038] The experiment was carried out in a 24-well plate, and the test plants of similar size were selected and put into the well plate, and the same amount of sterile water was added. The metabolites of strain F18 obtained in Example 2-3 were selected and formulated into DMSO solutions of different concentrations, and the same volume was added to the orifice plate, and the addition of the same volume of DMSO was used as a negative control, and each treatment...

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Abstract

The present invention discloses Penicillium chrysogenum producing fungi having plant poison activity, a preparation method and applications thereof. The fungi is Penicillium Chrysogenum and has characteristics of radial wrinkles, white edge mycelium, velvet-like texture, a large number of conidium structure, blue-green color, yellowish exudate and soluble pigment, and slight tawny colony back surface. In addition, the metabolites of the fungi provide significant plant poison activity and nematode contact toxicity activity for other plants.

Description

technical field [0001] The invention relates to a fungus of the genus Penicillium chrysogenum with phytotoxicity and a preparation method and application thereof, belonging to the technical field of microbial pesticides. Background technique [0002] According to the Food and Agriculture Organization of the United Nations, there are about 50,000 species of weeds in the world, including 8,000 species of weeds in farmland. Weeds survive long-term adaptation to local crops, cultivation, cultivation, soil, climate and other ecological and social conditions, and they harm crops in many ways. They compete with crops for water, fertilizer, light, etc., occupy aboveground and underground space, affect crop photosynthesis, interfere with crop growth, and affect yield and quality. In addition, many weeds are intermediate hosts of germs and pests that harm crops. For example, barnyardgrass is an intermediate host for rice planthoppers, rice leafhoppers, and armyworms. Weeds are the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P5/00C12R1/82
CPCC12N1/14C12N1/145C12R2001/82
Inventor 刘权秦波金辉
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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